Starch nanoparticles (sNPs) are attractive for numerous applications due to their non-toxicity, environmentallyfriendly nature, readily available raw materials worldwide, and biodegradability. However, the heterogeneousnature of starch makes it a challenge to prepare homogeneous sNP. The lack of existing preparation methods forsmall-sized sNPs (ss-sNPs) with high quality has been limiting their practical applications. In this study, we havedeveloped a new method by combining nanoprecipitation and successive centrifugation to generate ss-sNPs withwell-defined properties from six different starch types. The method is simple, environmentally friendly, requiresa relatively short processing time (<4 h) and generated sizes smaller than 50 nm diameter. The products werethoroughly investigated, structurally, microscopically, and physically. The ss-sNPs products derived from allstarch types demonstrated high stability in water (up to three weeks), meeting the requirements for practicallyany application. The formation of near monodisperse 10 nm diameter ss-sNPs was achieved using a high amylose(more linear) starch type as starting material. However, all ss-sNPs were mainly comprised of short-chainedamylopectin. This general and efficient method for producing ss-sNPs offers significant opportunities for theirapplication in various fields.