Edible mycelium bioengineered for enhanced nutritional value and sensory appeal using a modular synthetic biology toolkit

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Standard

Edible mycelium bioengineered for enhanced nutritional value and sensory appeal using a modular synthetic biology toolkit. / Maini Rekdal, Vayu; van der Luijt, Casper R.B.; Chen, Yan; Kakumanu, Ramu; Baidoo, Edward E.K.; Petzold, Christopher J.; Cruz-Morales, Pablo; Keasling, Jay D.

I: Nature Communications, Bind 15, 2099, 2024.

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt

Harvard

Maini Rekdal, V, van der Luijt, CRB, Chen, Y, Kakumanu, R, Baidoo, EEK, Petzold, CJ, Cruz-Morales, P & Keasling, JD 2024, 'Edible mycelium bioengineered for enhanced nutritional value and sensory appeal using a modular synthetic biology toolkit', Nature Communications, bind 15, 2099. https://doi.org/10.1038/s41467-024-46314-8

APA

Maini Rekdal, V., van der Luijt, C. R. B., Chen, Y., Kakumanu, R., Baidoo, E. E. K., Petzold, C. J., Cruz-Morales, P., & Keasling, J. D. (2024). Edible mycelium bioengineered for enhanced nutritional value and sensory appeal using a modular synthetic biology toolkit. Nature Communications, 15, [2099]. https://doi.org/10.1038/s41467-024-46314-8

Vancouver

Maini Rekdal V, van der Luijt CRB, Chen Y, Kakumanu R, Baidoo EEK, Petzold CJ o.a. Edible mycelium bioengineered for enhanced nutritional value and sensory appeal using a modular synthetic biology toolkit. Nature Communications. 2024;15. 2099. https://doi.org/10.1038/s41467-024-46314-8

Author

Maini Rekdal, Vayu ; van der Luijt, Casper R.B. ; Chen, Yan ; Kakumanu, Ramu ; Baidoo, Edward E.K. ; Petzold, Christopher J. ; Cruz-Morales, Pablo ; Keasling, Jay D. / Edible mycelium bioengineered for enhanced nutritional value and sensory appeal using a modular synthetic biology toolkit. I: Nature Communications. 2024 ; Bind 15.

Bibtex

@article{563eabdf540144c7baa4a8448051895e,
title = "Edible mycelium bioengineered for enhanced nutritional value and sensory appeal using a modular synthetic biology toolkit",
abstract = "Filamentous fungi are critical in the transition to a more sustainable food system. While genetic modification of these organisms has promise for enhancing the nutritional value, sensory appeal, and scalability of fungal foods, genetic tools and demonstrated use cases for bioengineered food production by edible strains are lacking. Here, we develop a modular synthetic biology toolkit for Aspergillus oryzae, an edible fungus used in fermented foods, protein production, and meat alternatives. Our toolkit includes a CRISPR-Cas9 method for gene integration, neutral loci, and tunable promoters. We use these tools to elevate intracellular levels of the nutraceutical ergothioneine and the flavor-and color molecule heme in the edible biomass. The strain overproducing heme is red in color and is readily formulated into imitation meat patties with minimal processing. These findings highlight the promise of synthetic biology to enhance fungal foods and provide useful genetic tools for applications in food production and beyond.",
author = "{Maini Rekdal}, Vayu and {van der Luijt}, {Casper R.B.} and Yan Chen and Ramu Kakumanu and Baidoo, {Edward E.K.} and Petzold, {Christopher J.} and Pablo Cruz-Morales and Keasling, {Jay D.}",
note = "Publisher Copyright: {\textcopyright} The Author(s) 2024.",
year = "2024",
doi = "10.1038/s41467-024-46314-8",
language = "English",
volume = "15",
journal = "Nature Communications",
issn = "2041-1723",
publisher = "nature publishing group",

}

RIS

TY - JOUR

T1 - Edible mycelium bioengineered for enhanced nutritional value and sensory appeal using a modular synthetic biology toolkit

AU - Maini Rekdal, Vayu

AU - van der Luijt, Casper R.B.

AU - Chen, Yan

AU - Kakumanu, Ramu

AU - Baidoo, Edward E.K.

AU - Petzold, Christopher J.

AU - Cruz-Morales, Pablo

AU - Keasling, Jay D.

N1 - Publisher Copyright: © The Author(s) 2024.

PY - 2024

Y1 - 2024

N2 - Filamentous fungi are critical in the transition to a more sustainable food system. While genetic modification of these organisms has promise for enhancing the nutritional value, sensory appeal, and scalability of fungal foods, genetic tools and demonstrated use cases for bioengineered food production by edible strains are lacking. Here, we develop a modular synthetic biology toolkit for Aspergillus oryzae, an edible fungus used in fermented foods, protein production, and meat alternatives. Our toolkit includes a CRISPR-Cas9 method for gene integration, neutral loci, and tunable promoters. We use these tools to elevate intracellular levels of the nutraceutical ergothioneine and the flavor-and color molecule heme in the edible biomass. The strain overproducing heme is red in color and is readily formulated into imitation meat patties with minimal processing. These findings highlight the promise of synthetic biology to enhance fungal foods and provide useful genetic tools for applications in food production and beyond.

AB - Filamentous fungi are critical in the transition to a more sustainable food system. While genetic modification of these organisms has promise for enhancing the nutritional value, sensory appeal, and scalability of fungal foods, genetic tools and demonstrated use cases for bioengineered food production by edible strains are lacking. Here, we develop a modular synthetic biology toolkit for Aspergillus oryzae, an edible fungus used in fermented foods, protein production, and meat alternatives. Our toolkit includes a CRISPR-Cas9 method for gene integration, neutral loci, and tunable promoters. We use these tools to elevate intracellular levels of the nutraceutical ergothioneine and the flavor-and color molecule heme in the edible biomass. The strain overproducing heme is red in color and is readily formulated into imitation meat patties with minimal processing. These findings highlight the promise of synthetic biology to enhance fungal foods and provide useful genetic tools for applications in food production and beyond.

U2 - 10.1038/s41467-024-46314-8

DO - 10.1038/s41467-024-46314-8

M3 - Journal article

C2 - 38485948

AN - SCOPUS:85187897208

VL - 15

JO - Nature Communications

JF - Nature Communications

SN - 2041-1723

M1 - 2099

ER -

ID: 387876668