TY - JOUR

T1 - Plant derived ingredients rich in nitrates or phenolics for protection of pork against protein oxidation

AU - Martínez, Lorena

AU - Jongberg, Sisse

AU - Ros, Gaspar

AU - Skibsted, Leif H.

AU - Nieto, Gema

PY - 2020

Y1 - 2020

N2 - A pork model system containg phenolic extracts (citrus, rosemary, and acerola), traditional Spanish food ingredients (paprika, garlic, and oregano), or natural nitrate sources (beet, lettuce, arugula, spinach, chard, celery, and watercress) were oxidized by an hydrophilic (OXHydro, 2,2′-azobis(2-amidinopropane)-dihydrochloride; AAPH) or lipophilic (OXLip, 2,2′-azobis(2,4-dimethylvaleronitrile; AMVN) radical initiator. Citrus as well as lettuce and spinach protected almost fully against protein thiol loss and showed efficient radical scavenging activity as determined by ESR spectroscopy in both oxidizing systems. Rosemary was an efficient radical scavenger in both systems, but behaved as a prooxidant on thiols in the OXHydro system. Acerola was also found to be prooxidative as determined by increased radical signal intensity especially in the OXLip system, assigned to high concentration of ascorbate in the extract. Natural nitrate sources, especially lettuce and spinach, are accordingly potential substitutes for synthetic phenolic antioxidants protecting against protein thiol oxidation and radical formation in pork.

AB - A pork model system containg phenolic extracts (citrus, rosemary, and acerola), traditional Spanish food ingredients (paprika, garlic, and oregano), or natural nitrate sources (beet, lettuce, arugula, spinach, chard, celery, and watercress) were oxidized by an hydrophilic (OXHydro, 2,2′-azobis(2-amidinopropane)-dihydrochloride; AAPH) or lipophilic (OXLip, 2,2′-azobis(2,4-dimethylvaleronitrile; AMVN) radical initiator. Citrus as well as lettuce and spinach protected almost fully against protein thiol loss and showed efficient radical scavenging activity as determined by ESR spectroscopy in both oxidizing systems. Rosemary was an efficient radical scavenger in both systems, but behaved as a prooxidant on thiols in the OXHydro system. Acerola was also found to be prooxidative as determined by increased radical signal intensity especially in the OXLip system, assigned to high concentration of ascorbate in the extract. Natural nitrate sources, especially lettuce and spinach, are accordingly potential substitutes for synthetic phenolic antioxidants protecting against protein thiol oxidation and radical formation in pork.

KW - Antioxidant

KW - Meat model system

KW - Nitrates

KW - Pork

KW - Protein oxidation

KW - Thiol groups

U2 - 10.1016/j.foodres.2019.108789

DO - 10.1016/j.foodres.2019.108789

M3 - Journal article

C2 - 32036925

AN - SCOPUS:85076110878

VL - 129

JO - Food Research International

JF - Food Research International

SN - 0963-9969

M1 - 108789

ER -