Lactobacillus casei-fermented blueberry pomace augments sIgA production in high-fat diet mice by improving intestinal microbiota
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Lactobacillus casei-fermented blueberry pomace augments sIgA production in high-fat diet mice by improving intestinal microbiota. / Cheng, Yuxin; Tang, Shuxin; Huang, Yuting; Liang, Fuqiang; Fang, Yajing; Pan, Siyi; Wu, Ting; Xu, Xiaoyun.
I: Food & Function, Bind 11, Nr. 7, 2020, s. 6552-6564.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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TY - JOUR
T1 - Lactobacillus casei-fermented blueberry pomace augments sIgA production in high-fat diet mice by improving intestinal microbiota
AU - Cheng, Yuxin
AU - Tang, Shuxin
AU - Huang, Yuting
AU - Liang, Fuqiang
AU - Fang, Yajing
AU - Pan, Siyi
AU - Wu, Ting
AU - Xu, Xiaoyun
PY - 2020
Y1 - 2020
N2 - Intestinal secretory immunoglobulin A (sIgA)-improving function ofLactobacillus casei-fermented blueberry pomace (FBP) was investigated in this study. Male C57BL/6 mice were fed with control diet (CD) or high-fat diet (HFD) with or without FBP supplementation. Expressions of sIgA-associated genes/proteins were evaluated by quantitative polymerase chain reaction (qPCR), western blot and enzyme-linked immunosorbent assay (ELISA). Commensal microbiota in Peyer's patches (PPs) and caecal contents were analyzed by 16S rRNA Illumina sequencing and qPCR, respectively. FBP improved sIgA production in HFD mice at mRNA and protein levels.AkkermansiaandLactobacillusin PPs of HFD mice were statistically increased by FBP. Beneficial microbiota and short-chain fatty acids (SCFAs) in caecal contents were positively correlated with caecal immunoglobulins in HFD mice. FBP showed an ability to modulate intestinal microbiota, which improved sIgA production in HFD mice, warranting the potential use of berry by-products as functional ingredients in improving the intestinal immune barrier of HFD individuals.
AB - Intestinal secretory immunoglobulin A (sIgA)-improving function ofLactobacillus casei-fermented blueberry pomace (FBP) was investigated in this study. Male C57BL/6 mice were fed with control diet (CD) or high-fat diet (HFD) with or without FBP supplementation. Expressions of sIgA-associated genes/proteins were evaluated by quantitative polymerase chain reaction (qPCR), western blot and enzyme-linked immunosorbent assay (ELISA). Commensal microbiota in Peyer's patches (PPs) and caecal contents were analyzed by 16S rRNA Illumina sequencing and qPCR, respectively. FBP improved sIgA production in HFD mice at mRNA and protein levels.AkkermansiaandLactobacillusin PPs of HFD mice were statistically increased by FBP. Beneficial microbiota and short-chain fatty acids (SCFAs) in caecal contents were positively correlated with caecal immunoglobulins in HFD mice. FBP showed an ability to modulate intestinal microbiota, which improved sIgA production in HFD mice, warranting the potential use of berry by-products as functional ingredients in improving the intestinal immune barrier of HFD individuals.
KW - GUT MICROBIOTA
KW - SECRETORY IGA
KW - IMMUNOGLOBULIN-A
KW - INDUCED OBESITY
KW - PEYERS-PATCHES
KW - T-CELLS
KW - SUPPLEMENTATION
KW - HYDROXYTYROSOL
KW - CONSUMPTION
KW - LIVER
U2 - 10.1039/d0fo01119c
DO - 10.1039/d0fo01119c
M3 - Journal article
C2 - 32643709
VL - 11
SP - 6552
EP - 6564
JO - Food & Function
JF - Food & Function
SN - 2042-6496
IS - 7
ER -
ID: 246729659