Gut microbiome and metabolome of sea cucumber (Stichopus ocellatus) as putative markers for monitoring the marine sediment pollution in Pahang, Malaysia
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Gut microbiome and metabolome of sea cucumber (Stichopus ocellatus) as putative markers for monitoring the marine sediment pollution in Pahang, Malaysia. / Wei, Siew Shing; Yen, Choo Mei; Marshall, Ian P.G.; Hamid, Hazrulrizawati Abd; Kamal, Shamrulazhar Shamzir; Nielsen, Dennis Sandris; Ahmad, Hajar Fauzan.
I: Marine Pollution Bulletin, Bind 182, 114022, 2022.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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TY - JOUR
T1 - Gut microbiome and metabolome of sea cucumber (Stichopus ocellatus) as putative markers for monitoring the marine sediment pollution in Pahang, Malaysia
AU - Wei, Siew Shing
AU - Yen, Choo Mei
AU - Marshall, Ian P.G.
AU - Hamid, Hazrulrizawati Abd
AU - Kamal, Shamrulazhar Shamzir
AU - Nielsen, Dennis Sandris
AU - Ahmad, Hajar Fauzan
N1 - Publisher Copyright: © 2022 Elsevier Ltd
PY - 2022
Y1 - 2022
N2 - Antibiotic contamination in the marine environment forms an emerging threat to marine ecosystems. This study aimed to compare the gut and coelomic microbiota of Stichopus ocellatus with sediments between two coastal districts of Pahang, which potentially conferring as putative biomarkers for sediment pollution monitoring. The composition of the bacteria communities was determined using 16S rRNA V3-region gene amplicon sequencing, while hybrid whole-genome sequencing was employed to analyze the genome of Vibrio parahaemolyticus. The trace elements and antibiotic compositions were access using high-throughput spectrometry. The alpha- and beta-diversity of bacteria in gut and sediment samples from Kuantan differed substantially within (p-value = 0.017604) and between samples (p-value <0.007), respectively. Vibrio genera predominated in Kuantan samples, while Flavobacterium and Synechococcus_E genera predominated in Pekan samples. Vibrio parahaemolyticus revealed the presence of tet(35) and blaCARB-33 genes that conceived resistance towards tetracycline and beta-lactam antibiotics, respectively, which were detected in sediment and gut samples.
AB - Antibiotic contamination in the marine environment forms an emerging threat to marine ecosystems. This study aimed to compare the gut and coelomic microbiota of Stichopus ocellatus with sediments between two coastal districts of Pahang, which potentially conferring as putative biomarkers for sediment pollution monitoring. The composition of the bacteria communities was determined using 16S rRNA V3-region gene amplicon sequencing, while hybrid whole-genome sequencing was employed to analyze the genome of Vibrio parahaemolyticus. The trace elements and antibiotic compositions were access using high-throughput spectrometry. The alpha- and beta-diversity of bacteria in gut and sediment samples from Kuantan differed substantially within (p-value = 0.017604) and between samples (p-value <0.007), respectively. Vibrio genera predominated in Kuantan samples, while Flavobacterium and Synechococcus_E genera predominated in Pekan samples. Vibrio parahaemolyticus revealed the presence of tet(35) and blaCARB-33 genes that conceived resistance towards tetracycline and beta-lactam antibiotics, respectively, which were detected in sediment and gut samples.
KW - Antibiotics
KW - Microbiome
KW - Pollution
KW - Sediment
KW - Stichopus ocellatus
KW - Vibrio parahaemolyticus
U2 - 10.1016/j.marpolbul.2022.114022
DO - 10.1016/j.marpolbul.2022.114022
M3 - Journal article
C2 - 35963228
AN - SCOPUS:85135687814
VL - 182
JO - Marine Pollution Bulletin
JF - Marine Pollution Bulletin
SN - 0025-326X
M1 - 114022
ER -
ID: 331589560