GC-MS based metabolomics and NMR spectroscopy investigation of food intake biomarkers for milk and cheese in serum of healthy humans
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GC-MS based metabolomics and NMR spectroscopy investigation of food intake biomarkers for milk and cheese in serum of healthy humans. / Trimigno, Alessia; Münger, Linda; Picone, Gianfranco; Freiburghaus, Carola; Pimentel, Grégory; Vionnet, Nathalie; Pralong, François; Capozzi, Francesco; Badertscher, René; Vergères, Guy.
I: Metabolites, Bind 8, Nr. 2, 26, 2018.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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TY - JOUR
T1 - GC-MS based metabolomics and NMR spectroscopy investigation of food intake biomarkers for milk and cheese in serum of healthy humans
AU - Trimigno, Alessia
AU - Münger, Linda
AU - Picone, Gianfranco
AU - Freiburghaus, Carola
AU - Pimentel, Grégory
AU - Vionnet, Nathalie
AU - Pralong, François
AU - Capozzi, Francesco
AU - Badertscher, René
AU - Vergères, Guy
PY - 2018
Y1 - 2018
N2 - The identification and validation of food intake biomarkers (FIBs) in human biofluids is a key objective for the evaluation of dietary intake. We report here the analysis of the GC-MS and 1H-NMR metabolomes of serum samples from a randomized cross-over study in 11 healthy volunteers having consumed isocaloric amounts of milk, cheese, and a soy drink as non-dairy alternative. Serum was collected at baseline, postprandially up to 6 h, and 24 h after consumption. A multivariate analysis of the untargeted serum metabolomes, combined with a targeted analysis of candidate FIBs previously reported in urine samples from the same study, identified galactitol, galactonate, and galactono-1,5-lactone (milk), 3-phenyllactic acid (cheese), and pinitol (soy drink) as candidate FIBs for these products. Serum metabolites not previously identified in the urine samples, e.g., 3-hydroxyisobutyrate after cheese intake, were detected. Finally, an analysis of the postprandial behavior of candidate FIBs, in particular the dairy fatty acids pentadecanoic acid and heptadecanoic acid, revealed specific kinetic patterns of relevance to their detection in future validation studies. Taken together, promising candidate FIBs for dairy intake appear to be lactose and metabolites thereof, for lactose-containing products, and microbial metabolites derived from amino acids, for fermented dairy products such as cheese.
AB - The identification and validation of food intake biomarkers (FIBs) in human biofluids is a key objective for the evaluation of dietary intake. We report here the analysis of the GC-MS and 1H-NMR metabolomes of serum samples from a randomized cross-over study in 11 healthy volunteers having consumed isocaloric amounts of milk, cheese, and a soy drink as non-dairy alternative. Serum was collected at baseline, postprandially up to 6 h, and 24 h after consumption. A multivariate analysis of the untargeted serum metabolomes, combined with a targeted analysis of candidate FIBs previously reported in urine samples from the same study, identified galactitol, galactonate, and galactono-1,5-lactone (milk), 3-phenyllactic acid (cheese), and pinitol (soy drink) as candidate FIBs for these products. Serum metabolites not previously identified in the urine samples, e.g., 3-hydroxyisobutyrate after cheese intake, were detected. Finally, an analysis of the postprandial behavior of candidate FIBs, in particular the dairy fatty acids pentadecanoic acid and heptadecanoic acid, revealed specific kinetic patterns of relevance to their detection in future validation studies. Taken together, promising candidate FIBs for dairy intake appear to be lactose and metabolites thereof, for lactose-containing products, and microbial metabolites derived from amino acids, for fermented dairy products such as cheese.
KW - Biomarker
KW - Cheese
KW - Metabolomics
KW - Milk
KW - Nutrition
KW - Serum metabolome
KW - Soy drink
UR - http://www.scopus.com/inward/record.url?scp=85046437335&partnerID=8YFLogxK
U2 - 10.3390/metabo8020026
DO - 10.3390/metabo8020026
M3 - Journal article
AN - SCOPUS:85046437335
VL - 8
JO - Metabolites
JF - Metabolites
SN - 2218-1989
IS - 2
M1 - 26
ER -
ID: 228368817