TY - JOUR

T1 - Urinary and plasma metabolome of farm mink (Neovison vison) after an intervention with raw or cooked poultry offal

T2 - a 1H NMR investigation

AU - Trimigno, Alessia

AU - Khakimov, Bekzod

AU - Quaade, Michelle Lauge

AU - Honoré, Oliver Legarth

AU - Clausen, Tove

AU - Blaabjerg, Karoline

AU - Engelsen, Søren Balling

AU - Hammer, Anne Sofie Vedsted

PY - 2022

Y1 - 2022

N2 - The introduction of high amounts of cooked poultry offal in mink feed has been associated with health problems in growing mink. Cooking mink feed is a convenient way of reducing microbiological activity, but it may have a negative effect on raw material quality and animal welfare. This study investigates growth and health of mink fed raw or cooked poultry offal and describes urinary and blood plasma metabolic changes related to the feeding. A total of 65 male mink were divided in three feeding groups, two fed cooked offal and one group fed raw offal, and the plasma and urine samples were collected at 3 time points during the growth. Both bio-fluids and feed samples were measured by H-1 NMR spectroscopy and resulted metabolomics data were analysed using univariate and multivariate statistical methods that revealed dominating effect of the mink growth stages and to a less extent the feeding regime. Metabolome differences in relation to low body mass index (BMI) and kidney lesions were observed in plasma. Disease and decrease in BMI was associated with high creatinine and dimethylglycine content in plasma. These molecules were also particularly indicative of the cooked feeds. Moreover, low urinary taurine levels were also associated with disease and low BMI. Individual mink appeared to show negative effects of the cooked feed diet, including impaired growth and gross pathological lesions involving the kidneys. This may be related to the absorption of essential metabolites such as amino acids and fats, necessary for mink growth, that are negatively impacted by the cooking process.

AB - The introduction of high amounts of cooked poultry offal in mink feed has been associated with health problems in growing mink. Cooking mink feed is a convenient way of reducing microbiological activity, but it may have a negative effect on raw material quality and animal welfare. This study investigates growth and health of mink fed raw or cooked poultry offal and describes urinary and blood plasma metabolic changes related to the feeding. A total of 65 male mink were divided in three feeding groups, two fed cooked offal and one group fed raw offal, and the plasma and urine samples were collected at 3 time points during the growth. Both bio-fluids and feed samples were measured by H-1 NMR spectroscopy and resulted metabolomics data were analysed using univariate and multivariate statistical methods that revealed dominating effect of the mink growth stages and to a less extent the feeding regime. Metabolome differences in relation to low body mass index (BMI) and kidney lesions were observed in plasma. Disease and decrease in BMI was associated with high creatinine and dimethylglycine content in plasma. These molecules were also particularly indicative of the cooked feeds. Moreover, low urinary taurine levels were also associated with disease and low BMI. Individual mink appeared to show negative effects of the cooked feed diet, including impaired growth and gross pathological lesions involving the kidneys. This may be related to the absorption of essential metabolites such as amino acids and fats, necessary for mink growth, that are negatively impacted by the cooking process.

KW - Metabolomics

KW - mink

KW - Neovison vison

KW - NMR spectroscopy

KW - poultry offal

KW - processing

KW - protein digestibility

KW - MUSTELA-VISON

KW - SOYBEAN-MEAL

KW - AMINO-ACIDS

KW - NMR

KW - DIGESTIBILITY

KW - H-1

KW - SPECTROSCOPY

KW - COMBINATION

KW - BIOMARKERS

KW - EXCRETION

U2 - 10.1080/1745039X.2021.2003682

DO - 10.1080/1745039X.2021.2003682

M3 - Journal article

C2 - 35289194

VL - 76

SP - 74

EP - 91

JO - Archives of Animal Nutrition

JF - Archives of Animal Nutrition

SN - 1745-039X

IS - 1

ER -