Genetic manipulation of structural color in bacterial colonies
Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
Naturally occurring photonic structures are responsible for the bright and vivid coloration in a large variety of living organisms. Despite efforts to understand their biological functions, development, and complex optical response, little is known of the underlying genes involved in the development of these nanostructures in any domain of life. Here, we used Flavobacterium colonies as a model system to demonstrate that genes responsible for gliding motility, cell shape, the stringent response, and tRNA modification contribute to the optical appearance of the colony. By structural and optical analysis, we obtained a detailed correlation of how genetic modifications alter structural color in bacterial colonies. Understanding of genotype and phenotype relations in this system opens the way to genetic engineering of on-demand living optical materials, for use as paints and living sensors.
| Originalsprog | Engelsk |
|---|---|
| Tidsskrift | Proceedings of the National Academy of Sciences of the United States of America |
| Vol/bind | 115 |
| Udgave nummer | 11 |
| Sider (fra-til) | 2652-2657 |
| Antal sider | 6 |
| ISSN | 0027-8424 |
| DOI | |
| Status | Udgivet - 2018 |
Bibliografisk note
Funding Information:
ACKNOWLEDGMENTS. C.J.I. thanks Philip de Groot and students and staff of Hogeschool Utrecht, Biobased Economy and Leiden for bioinformatics support and Marcel Giesbers for help with electron microscopy. C.J.I. thanks Mark McBride and Yongtao Zhu for assistance with transposon mutagenesis. V.E.J., S.V., and T.S.R. thank Lars Jelsbak for fruitful discussions. B.D.W. and S.V. thank Tobias Wenzel for discussions. V.E.J., M.M.S., and S.V. thank Bev-erley Glover for help with growing bacteria. C.J.I. thanks the Biotechnology-Based Ecologically Balanced Sustainable Industrial Consortium (BE-Basic) Foundation (The Netherlands) for financial support. V.E.J., S.V., and T.S.R. thank the Biotechnology and Biological Sciences Research Council (BBSRC) David Phillips fellowship (BB/K014617/1), the European Research Council (ERC-2014-STG H2020 639088), and the European Commission [Marie Curie Fellowship Looking Through Disorder (LODIS), 701455] for financial support. B.D.W. was financially supported through the National Center of Competence in Research Bio-Inspired Materials and the Ambizione program of the Swiss National Science Foundation (168223).
Publisher Copyright:
© 2018 National Academy of Sciences. All Rights Reserved.
ID: 380650838