Riboflavin and chlorophyll as photosensitizers in electroformed giant unilamellar vesicles as food models
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Riboflavin and chlorophyll as photosensitizers in electroformed giant unilamellar vesicles as food models. / Wang, Hui Jing; Liang, Ran; du, Hui Hui; Ai, Jing-Xuan; Han, Rui Min; Zhang, Jian Ping; Skibsted, Leif Horsfelt.
In: European Food Research and Technology, Vol. 243, No. 1, 2017, p. 21-26.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Riboflavin and chlorophyll as photosensitizers in electroformed giant unilamellar vesicles as food models
AU - Wang, Hui Jing
AU - Liang, Ran
AU - du, Hui Hui
AU - Ai, Jing-Xuan
AU - Han, Rui Min
AU - Zhang, Jian Ping
AU - Skibsted, Leif Horsfelt
PY - 2017
Y1 - 2017
N2 - Electroformed giant unilamellar vesicles (GUVs) were found to have optimal sizes (~10 µm average diameter) for studying effects of photosensitizers and antioxidants in lipid bilayers as food models. By using optical microscopy and digital image processing techniques, no membrane damage was found for hydrophilic riboflavin, while lipophilic chlorophyll a initiated GUV budding and subsequent disintegration under light irradiation, indicating that lipophilic photosensitizers are the more important in such structured lipids. Lipophilic β-carotene provided protection against oxidative damage induced by chlorophyll a as shown by an increased lag phase for budding; however, it had no effect on subsequent budding rate. Hydrophilic puerarin alone exhibited little protection in terms of lag phase, but decreased together with β-carotene budding rate after the lag phase by a factor of more than 2, showing a clear synergistic effect between a hydrophilic antioxidant, inactive in the aqueous phase alone, and a lipophilic antioxidant protecting lipids.
AB - Electroformed giant unilamellar vesicles (GUVs) were found to have optimal sizes (~10 µm average diameter) for studying effects of photosensitizers and antioxidants in lipid bilayers as food models. By using optical microscopy and digital image processing techniques, no membrane damage was found for hydrophilic riboflavin, while lipophilic chlorophyll a initiated GUV budding and subsequent disintegration under light irradiation, indicating that lipophilic photosensitizers are the more important in such structured lipids. Lipophilic β-carotene provided protection against oxidative damage induced by chlorophyll a as shown by an increased lag phase for budding; however, it had no effect on subsequent budding rate. Hydrophilic puerarin alone exhibited little protection in terms of lag phase, but decreased together with β-carotene budding rate after the lag phase by a factor of more than 2, showing a clear synergistic effect between a hydrophilic antioxidant, inactive in the aqueous phase alone, and a lipophilic antioxidant protecting lipids.
KW - Antioxidant synergism
KW - Giant unilamellar vesicle
KW - Lipid bilayer integrity
KW - Puerarin
KW - β-carotene
U2 - 10.1007/s00217-016-2718-9
DO - 10.1007/s00217-016-2718-9
M3 - Journal article
AN - SCOPUS:84970969118
VL - 243
SP - 21
EP - 26
JO - European Food Research and Technology
JF - European Food Research and Technology
SN - 1438-2377
IS - 1
ER -
ID: 171656444