Effect of chickpeas (Cicer arietinum) germination under minerals stress on the content of isoflavones and functional properties

Research output: Contribution to journalJournal articleResearchpeer-review

  • Raza, Husnain
  • Hafiz Rizwan Sharif
  • Farah Zaaboul
  • Muhammad Shoaib
  • Waleed Aboshora
  • Barkat Ali
  • Lianfu Zhang

Germination of chickpeas was carried out in the light using a closed germination chamber at 25 ± 2°C and 80% R.H. Calcium, zinc and selenium were sprayed throughout the germination period in concentrations of 25, 50, 75, and 100mg/L. HPLC-UV was used at 260nm to analyze the contents of isoflavones from the samples collected on 1st, 3rd, 5th, 7th and 9th day of germination. Profound increase in the content of biochanin A and fermonenetin was found on 7th day of germination in WM sample with 3.08 and 5.50 mg/g, respectively. Whereas, biochanin glucoside and genistein were acquired in maximum content of 4.34 and 2.41 mg/g in 5D25 and 9D25. The results proved the use of minerals at 25 and 50mg/L during germination to be ideal for increased isoflavone as well as for increased mineral contents. Significant increase of 0.98 g/mL, 1.27 g/g and 2.29 mL/g was observed for bulk density (BD), oil holding capacity (OHC) and water holding capacity (WHC), in a samples germinated for 5 days. The results of emulsifying activity (EA), foaming capacity (FC) and foaming stability (FS) significantly decreased from 44.22-36.48%, 27.82-12.63% and 16.34-8.00%, respectively. Current results of isoflavones, minerals and functional properties encourage the utilization of germinated chickpeas at industrial level for as a functional and nutritional ingredient for the germination period of 5 days at mineral supplementation of 25-50mg/L.

Original languageEnglish
JournalPakistan Journal of Agricultural Sciences
Volume57
Issue number2
Pages (from-to)591-598
Number of pages8
ISSN0552-9034
DOIs
Publication statusPublished - Mar 2020

Bibliographical note

Funding Information:
Acknowledgements: This study was supported by the grants from the State Key Research and Development Plan (No. 2017YFD0400200), the Key Research and Development Program of Jiangsu Province (BE2017374) and Jiangsu province “Collaborative Innovation Center for Food Safety and Quality Control” industry development program.

Publisher Copyright:
© 2020, University of Agriculture. All rights reserved.

    Research areas

  • Chickpeas, Functional properties, Germination, HPLC-UV, Isoflavones, Minerals

ID: 391502889