Biopolymer Based Food Delivery Systems - BIBAFOODS
An Initial Training Network (ITN) funded by the FP7-PEOPLE-2013 programme
PROJECT IS COMPLETED
Project period:1 February 2014 – 31 January 2018
The aim of BIBAFOODS is to train young researchers in developing colloidal delivery systems to protect and deliver active components via foods, resulting in novel functional foods.
Materials and coatings can be made responsive to the external chemical conditions and therefore suitable for controlled releases targeted at a desired stage during food processing or at a specific point during digestion of the food, e.g. in the intestinal tract. This will involve probiotic bacteria and enzymes that are liberated and allowed to be active in a controllable way. The ultimate successful materials ensure stability of the active component during long-term storage prior to food production, during food production or during digestion, but at the same time liberating the active component at the right point.
The behavior and interaction of the delivery systems will be studied by simulation of gastric and intestinal conditions and by implementation in food production and formulation into probiotic products.
The researchers trained in BIBAFOODS will obtain skills necessary for the development of the sustainable food industry, and they will be provided with entrepreneurial competences crucial for creating biotechnological food oriented start-up companies.
BIBAFOODS focuses on educating young researchers who can develop novel functional foods by use of colloidal delivery systems.
The researchers employed in BIBAFOODS will develop materials and coatings responsive to external chemical conditions, which are therefore suitable for controlled releases targeted at a desired stage during food processing or at a specific point during digestion of the food, e.g. in the intestinal tract.
More specifically, BIBAFOODS will concentrate on probiotic bacteria and enzymes that are liberated and allowed to be active in a controllable way.
The figure above shows a schematic representation of two different approaches to microencapsulate enzymes or microorganisms. Microorganisms could be self-organized and subsequently coated with a responsive layer (left) or enzymes/microorganisms could be embedded in a matrix further coated with a responsive layer (right).
The success of developed materials will be evaluated against the following criteria:
- Timing of liberation of the active component
There are 11 partners in BIBAFOODS, including universities, a public sector research organization, and private companies:
- University of Copenhagen (Denmark)
- Chr. Hansen A/S (Denmark)
- Lund University (Sweden)
- CR Competence AB (Sweden)
- Heidelberg University (Germany)
- ProDigest (Belgium)
- Ghent University (Belgium)
- University of Lorraine (France)
- University of Alcalá (Spain)
- The Spanish National Research Council (Spain)
- University of Coimbra (Portugal)
In addition, 5 associated partners take part in the network: Aventure AB (Sweden), ThermoScientific (Spain), Malmö University (Sweden), Algarve University (Portugal) and University of Barcelona (Spain).
During 2014 and 2015 BIBAFOODS hired 11 early stage (PhD students) and 3 experienced researchers (Post Docs).
ESR 1: Ileana Alexandra Pavel. Project title: Silica based materials for encapsulation of enzymes. University of Lorraine, France. Supervisors: Andreea Pasc, Nadia Canilho, Jordi Esquena (CSIC).
ESR 2: Yoran Beldengrün. Project title: Cross-linked hydrogels as delivery systems for enzymes. CSIC, Spain. Main supervisor: Jordi Esquena.
ESR 3: Maria Valldeperas Badell. Project title: Lipid based nanostructures for entrapment of enzymes. University of Lund, Sweden. Main supervisor: Tommy Nylander
ESR 4: Federico Amadei. Project title: Fabrication and characterization of polymer-based capsules for probiotics. University of Heidelberg, Germany. Supervisors: Motomu Tanaka, Stefan Kaufmann.
ESR 5: Fernanda Bianca Haffner. Project title: Encapsulation of probiotics within polymer beads-templated hierarchical silica. University of Lorraine, France. Supervisors: Andreea Pasc, Roudayna Diab, Maria Graca Miguel (University of Coimbra).
ESR 6: Poonam Singh. Project title: Cellulose based Hydrogels for probiotic encapsulation. University of Coimbra, Portugal. Supervisors: Björn Lindman, Bruno Medronho (University of Algarve), Jordi Esquena (CSIC).
ESR 7: Cigdem Yücel Falco. Project title: Coatings for individual and self-assembled probiotics. University of Copenhagen, Denmark. Supervisors: Jens Risbo, Marité Cardenas (Malmö University).
ESR 8: Sofia Prazéres and Diogo Quintela. Project title: Characterization of materials and their enzyme interactions by Raman Spectroscopy. Alcalá University, Spain. Supervisors: Gemma Montalvo Garcia, Carmen Garcia, Andreea Pasc (University of Lorraine).
ESR 9: Tomasz Cieplak. Project title: Application of advanced GIT methodology for product validation and assessment of product stability. University of Copenhagen, Denmark. Supervisors: Dennis S. Nielsen, Franciskus W. J. van der Berg, Tom van de Wiele (University of Ghent)
ESR 10: Racha el Hage. Project title: Establishment of a stable multi-species probiotic consortium through hydrogel encapsulation techniques. University of Ghent, Belgium. Main supervisor: Tom van de Wiele.
ESR 11: Maryam Eshrati. Project title: Material stability during gastrointestinal transit and at the gut mucosa. University of Heidelberg, Germany. Supervisors: Stefan Kaufmann, Motomu Tanaka.
ER1: Federica Sebastiani. Project title: Controlled release vehicles based on functionalized alkylglykosides. CR Competence AB, Sweden. Supervisor: Stefan Ulvenlund.
ER2: Surender Kumar Dhayal. Project title: Coating for microcapsules in cheese ripening. Chr Hansen A/S, Denmark. Supervisor: Hans van den Brink.
ER3: Davide Gottardi. Project title: Enzyme and probiotic stability and functionality assessment at the gut mucosa. ProDigest AB, Belgium. Supervisor: Sam Possemiers.
Videos about the individual projects in BIBAFOODS:
A.P. Dabkowska, C. Hirst, M. Valldeperas, L.A. Clifton, C. Montis, S. Nöjd,
L. Gentile, M. Wang, G.K. Pálsson, S. Lages, D. Berti, J. Barauskas, T. Nylander
(2017): Temperature responsive lipid liquid crystal layers with embedded nanogels. Chem. Commun., 53, p. 1417-1420. DOI: 10.1039/C6CC09426K.
Roudayna Diab, Nadia Canilho, Ileana A. Pavel, Fernanda B. Haffner, Maxime Girardon, Andreea Pasc (2017); Silica-based systems for oral delivery of drugs, macromolecules and cells. Advances in Colloid and interface Science In press. DOI: http://dx.doi.org/10.1016/j.cis.2017.04.005
Cigdem Yucel Falco, Peter Falkman, Jens Risbo, Marité Cárdenas, Bruno Medronho (2017): Chitosan-dextran sulfate hydrogels as a potential carrier for probiotics. Carbohydrate Polymers, 172, p. 175-183. DOI: http://dx.doi.org/10.1016/j.carbpol.2017.04.047
Cigdem Yucel Falco, Xiaolu Geng, Marité Cárdenas, Jens Risbo (2017): Edible foam based on Pickering effect of probiotic bacteria and milk proteins. Food Hydrocolloids 70, p. 211–218. DOI: http://doi.org/10.1016/j.foodhyd.2017.04.003
Cigdem Yucel Falco, Javier Sotres, Ana Rascón, Jens Risbo, Marité Cárdenas (2017):
Design of a potentially prebiotic and responsive encapsulation material for probiotic bacteria based on chitosan and sulfated β-glucan. Journal of Colloid and Interface Science 487, p. 97-106. DOI: 10.1016/j.jcis.2016.10.019.
D. Gottardi, P. Van den Abbeele, M. Marzorati (2016): Use of the simulator of the human intestinal microbial ecosystem (SHIME®) to study the fate of food ingredients and actives. Agro FOOD Industry Hi Tech 27(5), p. XXII-XXIV.
Fernanda B. Haffner, Roudayna Diab, Andreea Pasc (2016): Encapsulation of probiotics: insights into academic and industrial approaches. AIMS Materials Science 3(1), p. 114-136. DOI: 10.3934/matersci.2016.1.114.
Fernanda B. Haffner, Maxime Girardon, Mathieu Etienne, Stephane Fontanay, Nadia Canilho, Raphael E Duval, Maciej Mierzwa, Roudayna Diab, Andreea Pasc (2016): Core-shell alginate@silica microparticles encapsulating probiotics. J. Mater. Chem. B 4, p. 7929-7935. DOI: 10.1039/C6TB02802K.
Agatha Korytowski, Wassim Abuillan, Federico Amadei, Ali Makky, Andrea Gumiero, Irmgard Sinning, Annika Gauss, Wolfgang Stremmel, Motomu Tanaka (2017): Accumulation of phosphatidylcholine on gut mucosal surface is not dominated by electrostatic interactions. Biochimica et Biophysica Acta 1859, p. 959-965. DOI: 10.1016/j.bbamem.2017.02.008.
Ileana-Alexandra Pavel, Sofia F. Prazeres, Gemma Montalvo, Carmen García Ruiz, Vincent Nicolas, Alain Celzard, Francois Dehez, Laetitia Canabady-Rochelle, Nadia Canilho, Andreea Pasc (2017): Effect of meso and macro size of hierarchical porous silica on the adsorption and activity of immobilized β-galactosidase. Langmuir 33, p. 3333-3340. DOI: 10.1021/acs.langmuir.7b00134.
Sofia Prazeres, Carmen García Ruiz and Gemma Montalvo García (2015): Vibrational Spectroscopy as Promising Tool to Study Enzyme-Carrier Interactions: A review. Applied Spectroscopy Reviews 50, p. 797-821. DOI: 10.1080/05704928.2015.1075207.
Carina Svendsen, Tomasz Cieplak, Frans W.J. van den Berg (2016): Exploring process dynamics by near infrared spectroscopy in lactic fermentations. Journal of Near Infrared Spectroscopy 24, p. 443-451. DOI: https://doi.org/10.1255/jnirs.1244.
Maria Valldeperas, Małgorzata Wiśniewska, Maor Ram-On, Ellina Kesselman, Dganit Danino, Tommy Nylander, and Justas Barauskas (2016): Sponge Phases and Nanoparticle Dispersions in Aqueous Mixtures of Mono- and Diglycerides. Langmuir 32(34), p. 8650−8659. DOI: 10.1021/acs.langmuir.6b01356
Poonam Singh, Hugo Duarte, Luís Alves, Filipe Antunes, Nicolas Le Moigne, Jan Dormanns, Benoît Duchemin, Mark P. Staiger and Bruno Medronho (2015). From Cellulose Dissolution and Regeneration to Added Value Applications — Synergism Between Molecular Understanding and Material Development, Cellulose - Fundamental Aspects and Current Trends, Dr. Matheus Poletto (Ed.), ISBN: 978-953-51-2229-6, InTech, DOI: 10.5772/61402.
Interview (in French) with Ileana Pavel and Fernanda Haffner in Factuel, a news website at University of Lorraine, 12 November 2014: http://factuel.univ-lorraine.fr/node/1924
Interview (in Spanish) with Sofia Prazeres, Gemma Montalvo and Carmen García Ruíz in ‘Diario Digital da Universidad de Alcalá’, 14th November 2014: http://www2.uah.es/diariodigital/index.php?option=com_content&task=view&id=8561&Itemid=34
The project was funded by:
This project has received funding from the European Union's Seventh Framework Programme for research, technological development and demonstration under grant agreement no 606713
Funding: EUR 3,765,084