TY - JOUR

T1 - The effect of sucrase-isomaltase deficiency on metabolism, food intake and preferences

T2 - protocol for a dietary intervention study

AU - Senftleber, Ninna Karsbæk

AU - Pedersen, Kristine Skøtt

AU - Schnoor Jørgensen, Cecilie

AU - Pedersen, Hanne

AU - Bjerg Christensen, Marie Mathilde

AU - Kabel Madsen, Emilie

AU - Andersen, Kristine

AU - Jørsboe, Emil

AU - Gillum, Matthew Paul

AU - Frøst, Michael Bom

AU - Hansen, Torben

AU - Jørgensen, Marit Eika

N1 - Funding Information: The study is funded by the Independent Research Fund Denmark (grant no. 1030-00363B), The Greenland Institute of Natural Resources (postdoctoral grant number 80.045), and The Greenlandic Research Council (research grant not numbered). Brugseni sponsored all foods for the dietary intervention and the ad libitum meal test. Publisher Copyright: © 2023 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.

PY - 2023

Y1 - 2023

N2 - In Greenland, traditional marine foods are increasingly being replaced by sucrose- and starch-rich foods. A knock-out c.273_274delAG variant in the sucrase-isomaltase (SI) gene is relatively common in Greenland, with homozygous carriers being unable to digest sucrose and some starch. The variant is associated with a healthier metabolic phenotype in Greenlanders, which is confirmed by SI-knockout mice. We aim to assess if the healthy phenotype is explained by metabolic and microbial differences and if food and taste preferences differ between SI-genotypes. This paper describes the protocol for a randomised cross-over trial conducted in Greenland in 2022 with two dietary interventions of three days; a traditional meat- and fish-rich diet and a starch-rich Western diet with 11 energy% sucrose. The power calculation showed that 22 homozygous SI-carriers and 22 non-carriers were sufficient to detect a 0.5 mmol/L difference in glycaemic variability (80% power, α=0.05). We enrolled 18 carriers and 20 non-carriers. We examined food preferences at baseline and collected samples before and after each intervention for metabolic, metabolome, and microbiome profiling. Analyses of samples have not been completed yet. The Ethics Committee of Greenland approved the study. Results will be disseminated in international peer-reviewed journals and to the general Greenlandic population. NCT05375656.

AB - In Greenland, traditional marine foods are increasingly being replaced by sucrose- and starch-rich foods. A knock-out c.273_274delAG variant in the sucrase-isomaltase (SI) gene is relatively common in Greenland, with homozygous carriers being unable to digest sucrose and some starch. The variant is associated with a healthier metabolic phenotype in Greenlanders, which is confirmed by SI-knockout mice. We aim to assess if the healthy phenotype is explained by metabolic and microbial differences and if food and taste preferences differ between SI-genotypes. This paper describes the protocol for a randomised cross-over trial conducted in Greenland in 2022 with two dietary interventions of three days; a traditional meat- and fish-rich diet and a starch-rich Western diet with 11 energy% sucrose. The power calculation showed that 22 homozygous SI-carriers and 22 non-carriers were sufficient to detect a 0.5 mmol/L difference in glycaemic variability (80% power, α=0.05). We enrolled 18 carriers and 20 non-carriers. We examined food preferences at baseline and collected samples before and after each intervention for metabolic, metabolome, and microbiome profiling. Analyses of samples have not been completed yet. The Ethics Committee of Greenland approved the study. Results will be disseminated in international peer-reviewed journals and to the general Greenlandic population. NCT05375656.

KW - Congenital sucrase-isomaltase deficiency

KW - dietary intervention

KW - food preferences

KW - greenland

KW - inuit diet

KW - metabolism

KW - microbiota

U2 - 10.1080/22423982.2023.2178067

DO - 10.1080/22423982.2023.2178067

M3 - Journal article

C2 - 38113483

AN - SCOPUS:85148658895

VL - 82

JO - International Journal of Circumpolar Health

JF - International Journal of Circumpolar Health

SN - 1239-9744

IS - 1

M1 - 2178067

ER -