Flow cytometric detection of wild yeast in lager breweries

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A flow cytometric method for detection of wild yeast infections in breweries is reported. It is based on selective enrichment in Malt extract Yeast extract Glucose Peptone broth (MYGP) at 37 degrees C and in MYGP with 200 ppm CuSO4 at 25 degrees C, staining with a fluorochrome precursor and flow cytometry. In experiments with several types of wild yeast isolated from breweries and two different strains of lager yeast it has been possible to detect one wild yeast per 10(6) culture yeast after 48-72 h of incubation and, in some cases, after 24 h.

Original languageEnglish
JournalInternational Journal of Food Microbiology
Issue number4
Pages (from-to)321-328
Number of pages8
Publication statusPublished - 1993
Externally publishedYes

    Research areas

  • Beer, Evaluation Studies as Topic, Flow Cytometry, Food Microbiology, Mycology, Pichia, Saccharomyces cerevisiae

ID: 131532094