Drug delivery by phospholipase A2 degradable liposomes
Research output: Contribution to journal › Journal article › Research › peer-review
The effect of poly(ethylene glycol)-phospholipid (PE-PEG) lipopolymers on phospholipase A2 (PLA2) hydrolysis of liposomes composed of stearoyl-oleoylphosphatidylcholine (SOPC) was investigated. The PLA2 lag-time, which is inversely related to the enzymatic activity, was determined by fluorescence, and the zeta-potentials of the liposomes were measured as a function of PE-PEG lipopolymer concentration. A significant decrease in the lag-time, and hence an increase in enzymatic activity, was observed with increasing amounts of the negatively charged PE-PEG lipopolymers incorporated into the SOPC liposomes. The enhancement of the PLA2 enzymatic activity might involve a stronger PLA2 binding affinity towards the negatively charged and polymer covered PEG liposomes.
|Journal||International Journal of Pharmaceutics|
|Number of pages||3|
|Publication status||Published - 19 Feb 2001|
- Degradation, Drug-delivery, Lipopolymer, Liposomes, PEG liposomes, Phospholipase A