Characteristics of fibers formed by cytochrome c and induced by anionic phospholipids

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

Characteristics of fibers formed by cytochrome c and induced by anionic phospholipids. / Alakoskela, Juha Matti; Jutila, Arimatti; Simonsen, Adam C.; Pirneskoski, Jussi; Pyhäjoki, Sinikka; Turunen, Raija; Marttila, Sani; Mouritsen, Ole G.; Goormaghtigh, Erik; Kinnunen, Paavo K.J.

In: Biochemistry, Vol. 45, No. 45, 14.11.2006, p. 13447-13453.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Alakoskela, JM, Jutila, A, Simonsen, AC, Pirneskoski, J, Pyhäjoki, S, Turunen, R, Marttila, S, Mouritsen, OG, Goormaghtigh, E & Kinnunen, PKJ 2006, 'Characteristics of fibers formed by cytochrome c and induced by anionic phospholipids', Biochemistry, vol. 45, no. 45, pp. 13447-13453. https://doi.org/10.1021/bi0611107

APA

Alakoskela, J. M., Jutila, A., Simonsen, A. C., Pirneskoski, J., Pyhäjoki, S., Turunen, R., Marttila, S., Mouritsen, O. G., Goormaghtigh, E., & Kinnunen, P. K. J. (2006). Characteristics of fibers formed by cytochrome c and induced by anionic phospholipids. Biochemistry, 45(45), 13447-13453. https://doi.org/10.1021/bi0611107

Vancouver

Alakoskela JM, Jutila A, Simonsen AC, Pirneskoski J, Pyhäjoki S, Turunen R et al. Characteristics of fibers formed by cytochrome c and induced by anionic phospholipids. Biochemistry. 2006 Nov 14;45(45):13447-13453. https://doi.org/10.1021/bi0611107

Author

Alakoskela, Juha Matti ; Jutila, Arimatti ; Simonsen, Adam C. ; Pirneskoski, Jussi ; Pyhäjoki, Sinikka ; Turunen, Raija ; Marttila, Sani ; Mouritsen, Ole G. ; Goormaghtigh, Erik ; Kinnunen, Paavo K.J. / Characteristics of fibers formed by cytochrome c and induced by anionic phospholipids. In: Biochemistry. 2006 ; Vol. 45, No. 45. pp. 13447-13453.

Bibtex

@article{604ff34e188c452e82a18e42f33c4790,
title = "Characteristics of fibers formed by cytochrome c and induced by anionic phospholipids",
abstract = "Recent publications described the formation of millimeter-length fibers by diverse lipid-binding proteins (e.g., histone H1, cytochrome c, indolicidin, and endostatin) when they are mixed with 80:20 phosphatidylcholine/ phosphatidylserine vesicles. Further, these fibers displayed amyloid characteristics when stained with Congo Red. In the study presented here, we found by FTIR the amide I absorption band to reveal significant variation in fibers formed by cytochrome c, with some consisting of cytochrome c in a nativelike conformation and some exhibiting strong amyloid (β-sheet) characteristics. Protein structure also varied from amyloid to nearly native within single fibers. Fibers were frequently blue or bluish and sometimes iridescent, likely due to interference of light in the fibers. The amyloid-type amide I band was observed for blue fibers only. AFM shows that fibers consist of smaller 3-4 nm diameter fibers with 10 nm lateral spacing.",
author = "Alakoskela, {Juha Matti} and Arimatti Jutila and Simonsen, {Adam C.} and Jussi Pirneskoski and Sinikka Pyh{\"a}joki and Raija Turunen and Sani Marttila and Mouritsen, {Ole G.} and Erik Goormaghtigh and Kinnunen, {Paavo K.J.}",
year = "2006",
month = nov,
day = "14",
doi = "10.1021/bi0611107",
language = "English",
volume = "45",
pages = "13447--13453",
journal = "Biochemistry",
issn = "0006-2960",
publisher = "American Chemical Society",
number = "45",

}

RIS

TY - JOUR

T1 - Characteristics of fibers formed by cytochrome c and induced by anionic phospholipids

AU - Alakoskela, Juha Matti

AU - Jutila, Arimatti

AU - Simonsen, Adam C.

AU - Pirneskoski, Jussi

AU - Pyhäjoki, Sinikka

AU - Turunen, Raija

AU - Marttila, Sani

AU - Mouritsen, Ole G.

AU - Goormaghtigh, Erik

AU - Kinnunen, Paavo K.J.

PY - 2006/11/14

Y1 - 2006/11/14

N2 - Recent publications described the formation of millimeter-length fibers by diverse lipid-binding proteins (e.g., histone H1, cytochrome c, indolicidin, and endostatin) when they are mixed with 80:20 phosphatidylcholine/ phosphatidylserine vesicles. Further, these fibers displayed amyloid characteristics when stained with Congo Red. In the study presented here, we found by FTIR the amide I absorption band to reveal significant variation in fibers formed by cytochrome c, with some consisting of cytochrome c in a nativelike conformation and some exhibiting strong amyloid (β-sheet) characteristics. Protein structure also varied from amyloid to nearly native within single fibers. Fibers were frequently blue or bluish and sometimes iridescent, likely due to interference of light in the fibers. The amyloid-type amide I band was observed for blue fibers only. AFM shows that fibers consist of smaller 3-4 nm diameter fibers with 10 nm lateral spacing.

AB - Recent publications described the formation of millimeter-length fibers by diverse lipid-binding proteins (e.g., histone H1, cytochrome c, indolicidin, and endostatin) when they are mixed with 80:20 phosphatidylcholine/ phosphatidylserine vesicles. Further, these fibers displayed amyloid characteristics when stained with Congo Red. In the study presented here, we found by FTIR the amide I absorption band to reveal significant variation in fibers formed by cytochrome c, with some consisting of cytochrome c in a nativelike conformation and some exhibiting strong amyloid (β-sheet) characteristics. Protein structure also varied from amyloid to nearly native within single fibers. Fibers were frequently blue or bluish and sometimes iridescent, likely due to interference of light in the fibers. The amyloid-type amide I band was observed for blue fibers only. AFM shows that fibers consist of smaller 3-4 nm diameter fibers with 10 nm lateral spacing.

UR - http://www.scopus.com/inward/record.url?scp=33751016055&partnerID=8YFLogxK

U2 - 10.1021/bi0611107

DO - 10.1021/bi0611107

M3 - Journal article

C2 - 17087498

AN - SCOPUS:33751016055

VL - 45

SP - 13447

EP - 13453

JO - Biochemistry

JF - Biochemistry

SN - 0006-2960

IS - 45

ER -

ID: 230977849