Characteristics of fibers formed by cytochrome c and induced by anionic phospholipids

Research output: Contribution to journalJournal article

  • Juha Matti Alakoskela
  • Arimatti Jutila
  • Adam C. Simonsen
  • Jussi Pirneskoski
  • Sinikka Pyhäjoki
  • Raija Turunen
  • Sani Marttila
  • Mouritsen, Ole G.
  • Erik Goormaghtigh
  • Paavo K.J. Kinnunen

Recent publications described the formation of millimeter-length fibers by diverse lipid-binding proteins (e.g., histone H1, cytochrome c, indolicidin, and endostatin) when they are mixed with 80:20 phosphatidylcholine/ phosphatidylserine vesicles. Further, these fibers displayed amyloid characteristics when stained with Congo Red. In the study presented here, we found by FTIR the amide I absorption band to reveal significant variation in fibers formed by cytochrome c, with some consisting of cytochrome c in a nativelike conformation and some exhibiting strong amyloid (β-sheet) characteristics. Protein structure also varied from amyloid to nearly native within single fibers. Fibers were frequently blue or bluish and sometimes iridescent, likely due to interference of light in the fibers. The amyloid-type amide I band was observed for blue fibers only. AFM shows that fibers consist of smaller 3-4 nm diameter fibers with 10 nm lateral spacing.

Original languageEnglish
JournalBiochemistry
Volume45
Issue number45
Pages (from-to)13447-13453
Number of pages7
ISSN0006-2960
DOIs
Publication statusPublished - 14 Nov 2006

ID: 230977849