Occurrence and identification of yeast species in fermented liquid feed for piglets
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Occurrence and identification of yeast species in fermented liquid feed for piglets. / Gori, Klaus; Bjørklund, Marina Kryger; Canibe, Nuria; Pedersen, Anni Øyan; Jespersen, Lene.
In: Microbial Ecology, Vol. 61, No. 1, 2011, p. 146-153.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Occurrence and identification of yeast species in fermented liquid feed for piglets
AU - Gori, Klaus
AU - Bjørklund, Marina Kryger
AU - Canibe, Nuria
AU - Pedersen, Anni Øyan
AU - Jespersen, Lene
PY - 2011
Y1 - 2011
N2 - The major objective of the present study was to investigate the occurrence and identity of yeast species in fermented liquid feed (FLF) used for feeding piglets. In total, 40 different Danish farms were included in the analysis. The preparation and composition of FLF was found to be very heterogeneous with high variations in both yeast counts and yeast species composition. The yeast population varied between 6.0 × 10(3) and 4.2 × 10(7) cfug(-1) with an average yeast count of 8.7 × 10(6) ± 1.1 × 10(7) cfug(-1). A total of 766 yeasts were isolated and identified by conventional and/or molecular typing techniques. The predominant yeast species in the FLF samples were found to be Candida milleri (58.4%), Kazachstania exigua (17.5%), Candida pararugosa (6.40%) and Kazachstania bulderi (5.09%). No clear separation between isolates of C. milleri and Candida humilis could be obtained based on sequencing of the D1/D2 region of the 26S rRNA gene. The combined use of ITS-RFLP analysis and phenotypic criteria did meanwhile suggest a closer relationship with C. milleri than C. humilis.
AB - The major objective of the present study was to investigate the occurrence and identity of yeast species in fermented liquid feed (FLF) used for feeding piglets. In total, 40 different Danish farms were included in the analysis. The preparation and composition of FLF was found to be very heterogeneous with high variations in both yeast counts and yeast species composition. The yeast population varied between 6.0 × 10(3) and 4.2 × 10(7) cfug(-1) with an average yeast count of 8.7 × 10(6) ± 1.1 × 10(7) cfug(-1). A total of 766 yeasts were isolated and identified by conventional and/or molecular typing techniques. The predominant yeast species in the FLF samples were found to be Candida milleri (58.4%), Kazachstania exigua (17.5%), Candida pararugosa (6.40%) and Kazachstania bulderi (5.09%). No clear separation between isolates of C. milleri and Candida humilis could be obtained based on sequencing of the D1/D2 region of the 26S rRNA gene. The combined use of ITS-RFLP analysis and phenotypic criteria did meanwhile suggest a closer relationship with C. milleri than C. humilis.
U2 - 10.1007/s00248-010-9706-6
DO - 10.1007/s00248-010-9706-6
M3 - Journal article
C2 - 20574824
VL - 61
SP - 146
EP - 153
JO - Microbial Ecology
JF - Microbial Ecology
SN - 0095-3628
IS - 1
ER -
ID: 32329160