Tracking alternative versions of the galactose gene network in the genus Saccharomyces and their expansion after domestication
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When Saccharomyces cerevisiae grows on mixtures of glucose and galactose, galactose utilization is repressed by glucose, and induction of the GAL gene network only occurs when glucose is exhausted. Contrary to reference GAL alleles, alternative alleles support faster growth on galactose, thus enabling distinct galactose utilization strategies maintained by balancing selection. Here, we report on new wild populations of Saccharomyces cerevisiae harboring alternative GAL versions and, for the first time, of Saccharomyces paradoxus alternative alleles. We also show that the non-functional GAL version found earlier in Saccharomyces kudriavzevii is phylogenetically related to the alternative versions, which constitutes a case of trans-specific maintenance of highly divergent alleles. Strains harboring the different GAL network variants show different levels of alleviation of glucose repression and growth proficiency on galactose. We propose that domestication involved specialization toward thriving in milk from a generalist ancestor partially adapted to galactose consumption in the plant niche.
| Originalsprog | Engelsk |
|---|---|
| Artikelnummer | 108987 |
| Tidsskrift | iScience |
| Vol/bind | 27 |
| Udgave nummer | 2 |
| Antal sider | 17 |
| ISSN | 2589-0042 |
| DOI | |
| Status | Udgivet - 2024 |
Bibliografisk note
Funding Information:
Feng-Yan Bai is gratefully acknowledged for making available two S. cerevisiae cultures from a wild Chinese population. This study was supported by Fundação para a Ciência e a Tecnologia (Portugal) grants UIDP/04378/2020 , UIDB/04378/2020 (UCIBIO), LA/P/0140/2020 (i4HB), PTDC/BIA-EVL/1100/2020 , and PTDC/BIA-EVL/0604/2021 . The computation work was carried out with support of INCD funded by FCT and FEDER under grants 22153-01/SAICT/2016 and 2023.09581.CPCA.A1 . Research in the Rokas lab is supported by the National Science Foundation ( DEB-2110404 ), the National Institutes of Health/National Institute of Allergy and Infectious Diseases ( R01 AI153356 ), and the Burroughs Wellcome Fund. Research in the Hittinger Lab was supported by the National Science Foundation under Grant No. DEB-2110403 , the USDA National Institute of Food and Agriculture (Hatch Project 1020204 and 7005101 ), in part by the DOE Great Lakes Bioenergy Research Center (DOE BER Office of Science DE–SC0018409 ) and an H. I. Romnes Faculty Fellowship (Office of the Vice Chancellor for Research and Graduate Education with funding from the Wisconsin Alumni Research Foundation ). I. Tsai was supported by National Science and Technology Council ( 111-2628-b-001-021 ), Taiwan. C. Rosa was funded by “ INCT Yeasts: Biodiversity, preservation and biotechnological innovation ,” funded by Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq), Brazil, grant # 406564/2022-1 , and CNPq process numbers 0457499/2014-1 , 313088/2020-9 , and 408733/2021 , and Fundação do Amparo a Pesquisa do Estado de Minas Gerais ( FAPEMIG , process numbers APQ-01525-14 and APQ-02552-15 ). Field work in West Africa by L. Jespersen was supported by DANIDA , the Danish International Development Assistance.
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© 2024 The Authors
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