Tracking alternative versions of the galactose gene network in the genus Saccharomyces and their expansion after domestication
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Tracking alternative versions of the galactose gene network in the genus Saccharomyces and their expansion after domestication. / Pontes, Ana; Paraíso, Francisca; Liu, Yu-Ching; Limtong, Savitree; Jindamorakot, Sasitorn; Jespersen, Lene; Gonçalves, Carla; Rosa, Carlos A.; Tsai, Isheng Jason; Rokas, Antonis; Hittinger, Chris Todd; Gonçalves, Paula; Sampaio, José Paulo.
I: iScience, Bind 27, Nr. 2, 108987, 2024.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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TY - JOUR
T1 - Tracking alternative versions of the galactose gene network in the genus Saccharomyces and their expansion after domestication
AU - Pontes, Ana
AU - Paraíso, Francisca
AU - Liu, Yu-Ching
AU - Limtong, Savitree
AU - Jindamorakot, Sasitorn
AU - Jespersen, Lene
AU - Gonçalves, Carla
AU - Rosa, Carlos A.
AU - Tsai, Isheng Jason
AU - Rokas, Antonis
AU - Hittinger, Chris Todd
AU - Gonçalves, Paula
AU - Sampaio, José Paulo
N1 - Publisher Copyright: © 2024 The Authors
PY - 2024
Y1 - 2024
N2 - When Saccharomyces cerevisiae grows on mixtures of glucose and galactose, galactose utilization is repressed by glucose, and induction of the GAL gene network only occurs when glucose is exhausted. Contrary to reference GAL alleles, alternative alleles support faster growth on galactose, thus enabling distinct galactose utilization strategies maintained by balancing selection. Here, we report on new wild populations of Saccharomyces cerevisiae harboring alternative GAL versions and, for the first time, of Saccharomyces paradoxus alternative alleles. We also show that the non-functional GAL version found earlier in Saccharomyces kudriavzevii is phylogenetically related to the alternative versions, which constitutes a case of trans-specific maintenance of highly divergent alleles. Strains harboring the different GAL network variants show different levels of alleviation of glucose repression and growth proficiency on galactose. We propose that domestication involved specialization toward thriving in milk from a generalist ancestor partially adapted to galactose consumption in the plant niche.
AB - When Saccharomyces cerevisiae grows on mixtures of glucose and galactose, galactose utilization is repressed by glucose, and induction of the GAL gene network only occurs when glucose is exhausted. Contrary to reference GAL alleles, alternative alleles support faster growth on galactose, thus enabling distinct galactose utilization strategies maintained by balancing selection. Here, we report on new wild populations of Saccharomyces cerevisiae harboring alternative GAL versions and, for the first time, of Saccharomyces paradoxus alternative alleles. We also show that the non-functional GAL version found earlier in Saccharomyces kudriavzevii is phylogenetically related to the alternative versions, which constitutes a case of trans-specific maintenance of highly divergent alleles. Strains harboring the different GAL network variants show different levels of alleviation of glucose repression and growth proficiency on galactose. We propose that domestication involved specialization toward thriving in milk from a generalist ancestor partially adapted to galactose consumption in the plant niche.
KW - Genetics
KW - Genomics
KW - Genotyping
U2 - 10.1016/j.isci.2024.108987
DO - 10.1016/j.isci.2024.108987
M3 - Journal article
C2 - 38333711
AN - SCOPUS:85185525315
VL - 27
JO - iScience
JF - iScience
SN - 2589-0042
IS - 2
M1 - 108987
ER -
ID: 384877586