Human Faecal 1H NMR Metabolomics: Evaluation of Solvent and Sample Processing on Coverage and Reproducibility of Signature Metabolites

Research output: Contribution to journalJournal articleResearchpeer-review

Standard

Human Faecal 1H NMR Metabolomics : Evaluation of Solvent and Sample Processing on Coverage and Reproducibility of Signature Metabolites. / Cui, Mengni; Trimigno, Alessia; Aru, Violetta; Khakimov, Bekzod; Engelsen, Søren Balling.

In: Analytical Chemistry, Vol. 92, No. 14, 2020, p. 9546-9555.

Research output: Contribution to journalJournal articleResearchpeer-review

Harvard

Cui, M, Trimigno, A, Aru, V, Khakimov, B & Engelsen, SB 2020, 'Human Faecal 1H NMR Metabolomics: Evaluation of Solvent and Sample Processing on Coverage and Reproducibility of Signature Metabolites', Analytical Chemistry, vol. 92, no. 14, pp. 9546-9555. https://doi.org/10.1021/acs.analchem.0c00606

APA

Cui, M., Trimigno, A., Aru, V., Khakimov, B., & Engelsen, S. B. (2020). Human Faecal 1H NMR Metabolomics: Evaluation of Solvent and Sample Processing on Coverage and Reproducibility of Signature Metabolites. Analytical Chemistry, 92(14), 9546-9555. https://doi.org/10.1021/acs.analchem.0c00606

Vancouver

Cui M, Trimigno A, Aru V, Khakimov B, Engelsen SB. Human Faecal 1H NMR Metabolomics: Evaluation of Solvent and Sample Processing on Coverage and Reproducibility of Signature Metabolites. Analytical Chemistry. 2020;92(14):9546-9555. https://doi.org/10.1021/acs.analchem.0c00606

Author

Cui, Mengni ; Trimigno, Alessia ; Aru, Violetta ; Khakimov, Bekzod ; Engelsen, Søren Balling. / Human Faecal 1H NMR Metabolomics : Evaluation of Solvent and Sample Processing on Coverage and Reproducibility of Signature Metabolites. In: Analytical Chemistry. 2020 ; Vol. 92, No. 14. pp. 9546-9555.

Bibtex

@article{1f6f757b8c3344c891885d7a7e3f663b,
title = "Human Faecal 1H NMR Metabolomics: Evaluation of Solvent and Sample Processing on Coverage and Reproducibility of Signature Metabolites",
abstract = "The human faecal metabolome is complex, but rich in information and allows investigation of the host metabolism as a function of diet and health. The faecal metabolome is still much less explored than the plasma and urine metabolome, and in order to generate comparable data across laboratories and cohorts, standard operating procedures are required. This study evaluates 10 protocols, using different extraction solvents and sample processing methods for measuring the human faecal metabolome using proton nuclear magnetic resonance (H-1 NMR) spectroscopy. Three solvents: water, methanol, and dimethyl sulfoxide (DMSO) were investigated at varying concentrations for their ability to extract metabolites directly from faecal slurry or after freeze-drying. The protocols were evaluated on four different pools of human feces. The study also demonstrates a novel signature mapping (SigMa) method for rapid and unbiased processing of complex NMR spectra applied for the first time to human faecal metabolomics. The method is provided with a library containing the chemical shift ranges of 81 common faecal metabolites for future unambiguous and rapid faecal metabolite annotations. The result from the 10 faecal extraction protocols were investigated in terms of reproducibility, coverage, and ability to extract low concentration metabolites. The solvent type was shown to induce the highest variation in the data (45.7{\%}) and the water based extractions allowed detection of the greatest number of metabolites and resulted in the highest reproducibility. Direct extraction of faecal slurry was proved to be more reproducible than freeze-drying. In addition, freeze-drying caused a relative loss of short chain fatty acids (SCFA). DMSO was used for the first time to extract faecal metabolites and enabled the detection of certain bile acids. Some derivatives of SCFA were only detected using methanol as solvent.",
keywords = "NMR-BASED METABOLOMICS, ULCERATIVE-COLITIS, SPECTROSCOPY, EXTRACTS, METABONOMICS, FECES, WATER, NORMALIZATION, MIXTURES, URINE",
author = "Mengni Cui and Alessia Trimigno and Violetta Aru and Bekzod Khakimov and Engelsen, {S{\o}ren Balling}",
year = "2020",
doi = "10.1021/acs.analchem.0c00606",
language = "English",
volume = "92",
pages = "9546--9555",
journal = "Analytical Chemistry",
issn = "0003-2700",
publisher = "American Chemical Society",
number = "14",

}

RIS

TY - JOUR

T1 - Human Faecal 1H NMR Metabolomics

T2 - Evaluation of Solvent and Sample Processing on Coverage and Reproducibility of Signature Metabolites

AU - Cui, Mengni

AU - Trimigno, Alessia

AU - Aru, Violetta

AU - Khakimov, Bekzod

AU - Engelsen, Søren Balling

PY - 2020

Y1 - 2020

N2 - The human faecal metabolome is complex, but rich in information and allows investigation of the host metabolism as a function of diet and health. The faecal metabolome is still much less explored than the plasma and urine metabolome, and in order to generate comparable data across laboratories and cohorts, standard operating procedures are required. This study evaluates 10 protocols, using different extraction solvents and sample processing methods for measuring the human faecal metabolome using proton nuclear magnetic resonance (H-1 NMR) spectroscopy. Three solvents: water, methanol, and dimethyl sulfoxide (DMSO) were investigated at varying concentrations for their ability to extract metabolites directly from faecal slurry or after freeze-drying. The protocols were evaluated on four different pools of human feces. The study also demonstrates a novel signature mapping (SigMa) method for rapid and unbiased processing of complex NMR spectra applied for the first time to human faecal metabolomics. The method is provided with a library containing the chemical shift ranges of 81 common faecal metabolites for future unambiguous and rapid faecal metabolite annotations. The result from the 10 faecal extraction protocols were investigated in terms of reproducibility, coverage, and ability to extract low concentration metabolites. The solvent type was shown to induce the highest variation in the data (45.7%) and the water based extractions allowed detection of the greatest number of metabolites and resulted in the highest reproducibility. Direct extraction of faecal slurry was proved to be more reproducible than freeze-drying. In addition, freeze-drying caused a relative loss of short chain fatty acids (SCFA). DMSO was used for the first time to extract faecal metabolites and enabled the detection of certain bile acids. Some derivatives of SCFA were only detected using methanol as solvent.

AB - The human faecal metabolome is complex, but rich in information and allows investigation of the host metabolism as a function of diet and health. The faecal metabolome is still much less explored than the plasma and urine metabolome, and in order to generate comparable data across laboratories and cohorts, standard operating procedures are required. This study evaluates 10 protocols, using different extraction solvents and sample processing methods for measuring the human faecal metabolome using proton nuclear magnetic resonance (H-1 NMR) spectroscopy. Three solvents: water, methanol, and dimethyl sulfoxide (DMSO) were investigated at varying concentrations for their ability to extract metabolites directly from faecal slurry or after freeze-drying. The protocols were evaluated on four different pools of human feces. The study also demonstrates a novel signature mapping (SigMa) method for rapid and unbiased processing of complex NMR spectra applied for the first time to human faecal metabolomics. The method is provided with a library containing the chemical shift ranges of 81 common faecal metabolites for future unambiguous and rapid faecal metabolite annotations. The result from the 10 faecal extraction protocols were investigated in terms of reproducibility, coverage, and ability to extract low concentration metabolites. The solvent type was shown to induce the highest variation in the data (45.7%) and the water based extractions allowed detection of the greatest number of metabolites and resulted in the highest reproducibility. Direct extraction of faecal slurry was proved to be more reproducible than freeze-drying. In addition, freeze-drying caused a relative loss of short chain fatty acids (SCFA). DMSO was used for the first time to extract faecal metabolites and enabled the detection of certain bile acids. Some derivatives of SCFA were only detected using methanol as solvent.

KW - NMR-BASED METABOLOMICS

KW - ULCERATIVE-COLITIS

KW - SPECTROSCOPY

KW - EXTRACTS

KW - METABONOMICS

KW - FECES

KW - WATER

KW - NORMALIZATION

KW - MIXTURES

KW - URINE

U2 - 10.1021/acs.analchem.0c00606

DO - 10.1021/acs.analchem.0c00606

M3 - Journal article

C2 - 32567838

VL - 92

SP - 9546

EP - 9555

JO - Analytical Chemistry

JF - Analytical Chemistry

SN - 0003-2700

IS - 14

ER -

ID: 247154532