Angiotensin-I converting enzyme inhibitory and antioxidant activity of bioactive peptides produced by enzymatic hydrolysis of skin from grass carp (Ctenopharyngodon idella)
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Angiotensin-I converting enzyme inhibitory and antioxidant activity of bioactive peptides produced by enzymatic hydrolysis of skin from grass carp (Ctenopharyngodon idella). / Yi, Jierong; De Gobba, Cristian; Skibsted, Leif Horsfelt; Otte, Jeanette.
In: International Journal of Food Properties, Vol. 20, No. 5, 2017, p. 1129-1144.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Angiotensin-I converting enzyme inhibitory and antioxidant activity of bioactive peptides produced by enzymatic hydrolysis of skin from grass carp (Ctenopharyngodon idella)
AU - Yi, Jierong
AU - De Gobba, Cristian
AU - Skibsted, Leif Horsfelt
AU - Otte, Jeanette
PY - 2017
Y1 - 2017
N2 - Grass carp skin pieces were homogenized in water and hydrolyzed by Alcalase®, collagenase, proteinase K, and/or trypsin at their optimum conditions. Samples were taken at various degrees of hydrolysis and were evaluated for antioxidant, antimicrobial, and angiotensin-converting enzyme inhibitory activities. Alcalase and collagenase completely hydrolyzed the skin with different rates, and released peptides with antioxidant and angiotensin-converting enzyme-inhibitory activity. These activities increased linearly with increasing degrees of hydrolysis. Subsequent incubation of the collagenase hydrolysates with trypsin slightly increased the antioxidant activity. Proteinase K, although only partially hydrolyzing the skin, also catalyzed the release of peptides with antioxidant and angiotensin-converting enzyme-inhibitory activities. These results show that skin by-products from grass carp can be a source of bioactive peptides produced by a one-step reaction. Such hydrolysates may be applied in food products to prolong shelf life and provide beneficial effects on blood pressure.
AB - Grass carp skin pieces were homogenized in water and hydrolyzed by Alcalase®, collagenase, proteinase K, and/or trypsin at their optimum conditions. Samples were taken at various degrees of hydrolysis and were evaluated for antioxidant, antimicrobial, and angiotensin-converting enzyme inhibitory activities. Alcalase and collagenase completely hydrolyzed the skin with different rates, and released peptides with antioxidant and angiotensin-converting enzyme-inhibitory activity. These activities increased linearly with increasing degrees of hydrolysis. Subsequent incubation of the collagenase hydrolysates with trypsin slightly increased the antioxidant activity. Proteinase K, although only partially hydrolyzing the skin, also catalyzed the release of peptides with antioxidant and angiotensin-converting enzyme-inhibitory activities. These results show that skin by-products from grass carp can be a source of bioactive peptides produced by a one-step reaction. Such hydrolysates may be applied in food products to prolong shelf life and provide beneficial effects on blood pressure.
KW - ACE-inhibition
KW - Antioxidant
KW - DH
KW - Enzymatic hydrolysis
KW - Grass carp skin
KW - Peptide profiles
U2 - 10.1080/10942912.2016.1203932
DO - 10.1080/10942912.2016.1203932
M3 - Journal article
AN - SCOPUS:84994158635
VL - 20
SP - 1129
EP - 1144
JO - International Journal of Food Properties
JF - International Journal of Food Properties
SN - 1094-2912
IS - 5
ER -
ID: 174594542