TWIST1 and chromatin regulatory proteins interact to guide neural crest cell differentiation

Research output: Contribution to journal › Journal article › Research › peer-review

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TWIST1 and chromatin regulatory proteins interact to guide neural crest cell differentiation. / Fan, Xiaochen; Pragathi Masamsetti, V.; Sun, Jane Q. J.; Engholm-Keller, Kasper; Osteil, Pierre; Studdert, Joshua; Graham, Mark E.; Fossat, Nicolas; Tam, Patrick P. L.

In: eLife, Vol. 10, e62873, 2021.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Fan, X, Pragathi Masamsetti, V, Sun, JQJ, Engholm-Keller, K, Osteil, P, Studdert, J, Graham, ME, Fossat, N & Tam, PPL 2021, 'TWIST1 and chromatin regulatory proteins interact to guide neural crest cell differentiation', eLife, vol. 10, e62873. https://doi.org/10.7554/eLife.62873

APA

Fan, X., Pragathi Masamsetti, V., Sun, J. Q. J., Engholm-Keller, K., Osteil, P., Studdert, J., Graham, M. E., Fossat, N., & Tam, P. P. L. (2021). TWIST1 and chromatin regulatory proteins interact to guide neural crest cell differentiation. eLife, 10, [e62873]. https://doi.org/10.7554/eLife.62873

Vancouver

Fan X, Pragathi Masamsetti V, Sun JQJ, Engholm-Keller K, Osteil P, Studdert J et al. TWIST1 and chromatin regulatory proteins interact to guide neural crest cell differentiation. eLife. 2021;10. e62873. https://doi.org/10.7554/eLife.62873

Author

Fan, Xiaochen ; Pragathi Masamsetti, V. ; Sun, Jane Q. J. ; Engholm-Keller, Kasper ; Osteil, Pierre ; Studdert, Joshua ; Graham, Mark E. ; Fossat, Nicolas ; Tam, Patrick P. L. / TWIST1 and chromatin regulatory proteins interact to guide neural crest cell differentiation. In: eLife. 2021 ; Vol. 10.

Bibtex

@article{3722c1afc2d3418c9e0125819282ba29,
title = "TWIST1 and chromatin regulatory proteins interact to guide neural crest cell differentiation",
abstract = "Protein interaction is critical molecular regulatory activity underlining cellular functions and precise cell fate choices. Using TWIST1 BioID-proximity-labelling and network propagation analyses, we discovered and characterized a TWIST-chromatin regulatory module (TWIST1-CRM) in the neural crest cells (NCC). Combinatorial perturbation of core members of TWIST1-CRM: TWIST1, CHD7, CHD8, and WHSC1 in cell models and mouse embryos revealed that loss of the function of the regulatory module resulted in abnormal differentiation of NCCs and compromised craniofacial tissue patterning. Following NCC delamination, low level of TWIST1-CRM activity is instrumental to stabilize the early NCC signatures and migratory potential by repressing the neural stem cell programs. High level of TWIST1 module activity at later phases commits the cells to the ectomesenchyme. Our study further revealed the functional interdependency of TWIST1 and potential neurocristopathy factors in NCC development.",
author = "Xiaochen Fan and {Pragathi Masamsetti}, V. and Sun, {Jane Q. J.} and Kasper Engholm-Keller and Pierre Osteil and Joshua Studdert and Graham, {Mark E.} and Nicolas Fossat and Tam, {Patrick P. L.}",
year = "2021",
doi = "10.7554/eLife.62873",
language = "English",
volume = "10",
journal = "eLife",
issn = "2050-084X",
publisher = "eLife Sciences Publications Ltd.",

}

RIS

TY - JOUR

T1 - TWIST1 and chromatin regulatory proteins interact to guide neural crest cell differentiation

AU - Fan, Xiaochen

AU - Pragathi Masamsetti, V.

AU - Sun, Jane Q. J.

AU - Engholm-Keller, Kasper

AU - Osteil, Pierre

AU - Studdert, Joshua

AU - Graham, Mark E.

AU - Fossat, Nicolas

AU - Tam, Patrick P. L.

PY - 2021

Y1 - 2021

N2 - Protein interaction is critical molecular regulatory activity underlining cellular functions and precise cell fate choices. Using TWIST1 BioID-proximity-labelling and network propagation analyses, we discovered and characterized a TWIST-chromatin regulatory module (TWIST1-CRM) in the neural crest cells (NCC). Combinatorial perturbation of core members of TWIST1-CRM: TWIST1, CHD7, CHD8, and WHSC1 in cell models and mouse embryos revealed that loss of the function of the regulatory module resulted in abnormal differentiation of NCCs and compromised craniofacial tissue patterning. Following NCC delamination, low level of TWIST1-CRM activity is instrumental to stabilize the early NCC signatures and migratory potential by repressing the neural stem cell programs. High level of TWIST1 module activity at later phases commits the cells to the ectomesenchyme. Our study further revealed the functional interdependency of TWIST1 and potential neurocristopathy factors in NCC development.

AB - Protein interaction is critical molecular regulatory activity underlining cellular functions and precise cell fate choices. Using TWIST1 BioID-proximity-labelling and network propagation analyses, we discovered and characterized a TWIST-chromatin regulatory module (TWIST1-CRM) in the neural crest cells (NCC). Combinatorial perturbation of core members of TWIST1-CRM: TWIST1, CHD7, CHD8, and WHSC1 in cell models and mouse embryos revealed that loss of the function of the regulatory module resulted in abnormal differentiation of NCCs and compromised craniofacial tissue patterning. Following NCC delamination, low level of TWIST1-CRM activity is instrumental to stabilize the early NCC signatures and migratory potential by repressing the neural stem cell programs. High level of TWIST1 module activity at later phases commits the cells to the ectomesenchyme. Our study further revealed the functional interdependency of TWIST1 and potential neurocristopathy factors in NCC development.

U2 - 10.7554/eLife.62873

DO - 10.7554/eLife.62873

M3 - Journal article

C2 - 33554859

AN - SCOPUS:85102008010

VL - 10

JO - eLife

JF - eLife

SN - 2050-084X

M1 - e62873

ER -

ID: 259040576