The Smallest Intestine (TSI) - a low volume in vitro model of the small intestine with increased throughput
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The Smallest Intestine (TSI) - a low volume in vitro model of the small intestine with increased throughput. / Cieplak, Tomasz; Wiese, M.; Nielsen, S.; Van De Wiele, T.; Van Den Berg, F.; Nielsen, D. S.
In: FEMS Microbiology Letters, Vol. 365, No. 21, fny231, 2018, p. 1-8.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - The Smallest Intestine (TSI) - a low volume in vitro model of the small intestine with increased throughput
AU - Cieplak, Tomasz
AU - Wiese, M.
AU - Nielsen, S.
AU - Van De Wiele, T.
AU - Van Den Berg, F.
AU - Nielsen, D. S.
PY - 2018
Y1 - 2018
N2 - There is a growing interest in understanding the fate and behaviour of probiotic microorganisms and bioactive compounds during passage of the human gastrointestinal tract (GIT). Here, we report the development of a small volume in vitro model called The smallest Intestine (TSI) with increased throughput focusing on simulating passage through the stomach and small intestine (SI). The basic TSI module consists of five reactors, with a working volume of 12 ml each. During the simulated passage through the SI, bile is absorbed and pH is adjusted to physiologically relevant values for duodenum, jejunum and ileum. A consortium of seven representative bacterial members of the ileum microbiota is included in the ileal stage of the model. The behaviour of three putative probiotic Lactobacillus strains during in vitro simulated upper GIT passage was tested in the model and results were compared to previous studies describing probiotic survival. It was found, that probiotic persistence is strongly related to whether food was ingested, but also to presence of the ileal microbiota, which significantly impacted probiotic survival. In conclusion, TSI allows testing a substantial number of samples, at low cost and short time, and is thus suitable as an in vitro screening platform.
AB - There is a growing interest in understanding the fate and behaviour of probiotic microorganisms and bioactive compounds during passage of the human gastrointestinal tract (GIT). Here, we report the development of a small volume in vitro model called The smallest Intestine (TSI) with increased throughput focusing on simulating passage through the stomach and small intestine (SI). The basic TSI module consists of five reactors, with a working volume of 12 ml each. During the simulated passage through the SI, bile is absorbed and pH is adjusted to physiologically relevant values for duodenum, jejunum and ileum. A consortium of seven representative bacterial members of the ileum microbiota is included in the ileal stage of the model. The behaviour of three putative probiotic Lactobacillus strains during in vitro simulated upper GIT passage was tested in the model and results were compared to previous studies describing probiotic survival. It was found, that probiotic persistence is strongly related to whether food was ingested, but also to presence of the ileal microbiota, which significantly impacted probiotic survival. In conclusion, TSI allows testing a substantial number of samples, at low cost and short time, and is thus suitable as an in vitro screening platform.
KW - digestion
KW - in vitro model
KW - probiotics
KW - screening
KW - small intestine
KW - stomach
U2 - 10.1093/femsle/fny231
DO - 10.1093/femsle/fny231
M3 - Journal article
C2 - 30247563
AN - SCOPUS:85055072785
VL - 365
SP - 1
EP - 8
JO - F E M S Microbiology Letters
JF - F E M S Microbiology Letters
SN - 0378-1097
IS - 21
M1 - fny231
ER -
ID: 210056016