Sporofaciens musculi gen. nov., sp. nov., a novel bacterium isolated from the caecum of an obese mouse

Research output: Contribution to journalJournal articlepeer-review

Standard

Sporofaciens musculi gen. nov., sp. nov., a novel bacterium isolated from the caecum of an obese mouse. / Rasmussen, Torben Sølbeck; Streidl, Theresa; Hitch, Thomas C. A.; Wortmann, Esther; Deptula, Paulina; Kofoed, Michael V. W.; Riedel, Thomas; Neumann-Schaal, Meina; Hansen, Michael; Nielsen, Dennis Sandris; Clavel, Thomas; Vogensen, Finn Kvist.

In: International Journal of Systematic and Evolutionary Microbiology, Vol. 71, No. 3, 004673, 2021.

Research output: Contribution to journalJournal articlepeer-review

Harvard

Rasmussen, TS, Streidl, T, Hitch, TCA, Wortmann, E, Deptula, P, Kofoed, MVW, Riedel, T, Neumann-Schaal, M, Hansen, M, Nielsen, DS, Clavel, T & Vogensen, FK 2021, 'Sporofaciens musculi gen. nov., sp. nov., a novel bacterium isolated from the caecum of an obese mouse', International Journal of Systematic and Evolutionary Microbiology, vol. 71, no. 3, 004673. https://doi.org/10.1099/ijsem.0.004673

APA

Rasmussen, T. S., Streidl, T., Hitch, T. C. A., Wortmann, E., Deptula, P., Kofoed, M. V. W., Riedel, T., Neumann-Schaal, M., Hansen, M., Nielsen, D. S., Clavel, T., & Vogensen, F. K. (2021). Sporofaciens musculi gen. nov., sp. nov., a novel bacterium isolated from the caecum of an obese mouse. International Journal of Systematic and Evolutionary Microbiology, 71(3), [004673]. https://doi.org/10.1099/ijsem.0.004673

Vancouver

Rasmussen TS, Streidl T, Hitch TCA, Wortmann E, Deptula P, Kofoed MVW et al. Sporofaciens musculi gen. nov., sp. nov., a novel bacterium isolated from the caecum of an obese mouse. International Journal of Systematic and Evolutionary Microbiology. 2021;71(3). 004673. https://doi.org/10.1099/ijsem.0.004673

Author

Rasmussen, Torben Sølbeck ; Streidl, Theresa ; Hitch, Thomas C. A. ; Wortmann, Esther ; Deptula, Paulina ; Kofoed, Michael V. W. ; Riedel, Thomas ; Neumann-Schaal, Meina ; Hansen, Michael ; Nielsen, Dennis Sandris ; Clavel, Thomas ; Vogensen, Finn Kvist. / Sporofaciens musculi gen. nov., sp. nov., a novel bacterium isolated from the caecum of an obese mouse. In: International Journal of Systematic and Evolutionary Microbiology. 2021 ; Vol. 71, No. 3.

Bibtex

@article{4e2bd3bc3ccb4a5da2525cdb4580e650,
title = "Sporofaciens musculi gen. nov., sp. nov., a novel bacterium isolated from the caecum of an obese mouse",
abstract = "A bacterial strain, designated WCA-9-b2T, was isolated from the caecal content of an 18-week-old obese C57BL/6NTac male mouse. According to phenotypic analyses, the isolate was rod-shaped, strictly anaerobic, spore-forming, non-motile and Gram-stain-positive, under the conditions tested. Colonies were irregular and non-pigmented. Analysis of the 16S rRNA gene sequence indicated that the isolate belonged to the order Clostridiales with Dorea longicatena ATCC 27755T (94.9 % sequence identity), Ruminococcus gnavus ATCC 29149T (94.8%) and Clostridium scindens ATCC 35704T (94.3%) being the closest relatives. Whole genome sequencing showed an average nucleotide identity <74.23 %, average amino acid identity <64.52–74.67 % and percentage of conserved proteins values <50 % against the nine closest relatives (D. longicatena, Ruminococcus gnavus, C. scindens, Dorea formicigenerans, Ruminococcus lactaris, Clostridium hylemonae, Merdimonas faecis, Faecalicatena contorta and Faecalicatena fissicatena). The genome-based G+C content of genomic DNA was 44.4 mol%. The major cellular fatty acids were C16: 0 (24.5%), C18: 1 cis9 (19.8 %), C16: 0 DMA (11.7%), C18: 0 (8.4%) and C14: 0 (6.6%). Respiratory quinones were not detected. The predominant metabolic end products of glucose fermentation were acetate and succinate. Production of CO2 and H2 were detected. Based on these data, we propose that strain WCA-9-b2T represents a novel species within a novel genus, for which the name Sporofaciens musculi gen. nov., sp. nov. is proposed. The type strain is WCA-9-b2T (=DSM 106039T=CECT 30156T).",
keywords = "Acetate, Diet-induced obesity, Firmicutes, Lachnospiraceae, Mouse faeces, Spore-forming, Succinate",
author = "Rasmussen, {Torben S{\o}lbeck} and Theresa Streidl and Hitch, {Thomas C. A.} and Esther Wortmann and Paulina Deptula and Kofoed, {Michael V. W.} and Thomas Riedel and Meina Neumann-Schaal and Michael Hansen and Nielsen, {Dennis Sandris} and Thomas Clavel and Vogensen, {Finn Kvist}",
year = "2021",
doi = "10.1099/ijsem.0.004673",
language = "English",
volume = "71",
journal = "International Journal of Systematic and Evolutionary Microbiology",
issn = "1466-5026",
publisher = "Society for General Microbiology",
number = "3",

}

RIS

TY - JOUR

T1 - Sporofaciens musculi gen. nov., sp. nov., a novel bacterium isolated from the caecum of an obese mouse

AU - Rasmussen, Torben Sølbeck

AU - Streidl, Theresa

AU - Hitch, Thomas C. A.

AU - Wortmann, Esther

AU - Deptula, Paulina

AU - Kofoed, Michael V. W.

AU - Riedel, Thomas

AU - Neumann-Schaal, Meina

AU - Hansen, Michael

AU - Nielsen, Dennis Sandris

AU - Clavel, Thomas

AU - Vogensen, Finn Kvist

PY - 2021

Y1 - 2021

N2 - A bacterial strain, designated WCA-9-b2T, was isolated from the caecal content of an 18-week-old obese C57BL/6NTac male mouse. According to phenotypic analyses, the isolate was rod-shaped, strictly anaerobic, spore-forming, non-motile and Gram-stain-positive, under the conditions tested. Colonies were irregular and non-pigmented. Analysis of the 16S rRNA gene sequence indicated that the isolate belonged to the order Clostridiales with Dorea longicatena ATCC 27755T (94.9 % sequence identity), Ruminococcus gnavus ATCC 29149T (94.8%) and Clostridium scindens ATCC 35704T (94.3%) being the closest relatives. Whole genome sequencing showed an average nucleotide identity <74.23 %, average amino acid identity <64.52–74.67 % and percentage of conserved proteins values <50 % against the nine closest relatives (D. longicatena, Ruminococcus gnavus, C. scindens, Dorea formicigenerans, Ruminococcus lactaris, Clostridium hylemonae, Merdimonas faecis, Faecalicatena contorta and Faecalicatena fissicatena). The genome-based G+C content of genomic DNA was 44.4 mol%. The major cellular fatty acids were C16: 0 (24.5%), C18: 1 cis9 (19.8 %), C16: 0 DMA (11.7%), C18: 0 (8.4%) and C14: 0 (6.6%). Respiratory quinones were not detected. The predominant metabolic end products of glucose fermentation were acetate and succinate. Production of CO2 and H2 were detected. Based on these data, we propose that strain WCA-9-b2T represents a novel species within a novel genus, for which the name Sporofaciens musculi gen. nov., sp. nov. is proposed. The type strain is WCA-9-b2T (=DSM 106039T=CECT 30156T).

AB - A bacterial strain, designated WCA-9-b2T, was isolated from the caecal content of an 18-week-old obese C57BL/6NTac male mouse. According to phenotypic analyses, the isolate was rod-shaped, strictly anaerobic, spore-forming, non-motile and Gram-stain-positive, under the conditions tested. Colonies were irregular and non-pigmented. Analysis of the 16S rRNA gene sequence indicated that the isolate belonged to the order Clostridiales with Dorea longicatena ATCC 27755T (94.9 % sequence identity), Ruminococcus gnavus ATCC 29149T (94.8%) and Clostridium scindens ATCC 35704T (94.3%) being the closest relatives. Whole genome sequencing showed an average nucleotide identity <74.23 %, average amino acid identity <64.52–74.67 % and percentage of conserved proteins values <50 % against the nine closest relatives (D. longicatena, Ruminococcus gnavus, C. scindens, Dorea formicigenerans, Ruminococcus lactaris, Clostridium hylemonae, Merdimonas faecis, Faecalicatena contorta and Faecalicatena fissicatena). The genome-based G+C content of genomic DNA was 44.4 mol%. The major cellular fatty acids were C16: 0 (24.5%), C18: 1 cis9 (19.8 %), C16: 0 DMA (11.7%), C18: 0 (8.4%) and C14: 0 (6.6%). Respiratory quinones were not detected. The predominant metabolic end products of glucose fermentation were acetate and succinate. Production of CO2 and H2 were detected. Based on these data, we propose that strain WCA-9-b2T represents a novel species within a novel genus, for which the name Sporofaciens musculi gen. nov., sp. nov. is proposed. The type strain is WCA-9-b2T (=DSM 106039T=CECT 30156T).

KW - Acetate

KW - Diet-induced obesity

KW - Firmicutes

KW - Lachnospiraceae

KW - Mouse faeces

KW - Spore-forming

KW - Succinate

U2 - 10.1099/ijsem.0.004673

DO - 10.1099/ijsem.0.004673

M3 - Journal article

C2 - 33512312

AN - SCOPUS:85103316653

VL - 71

JO - International Journal of Systematic and Evolutionary Microbiology

JF - International Journal of Systematic and Evolutionary Microbiology

SN - 1466-5026

IS - 3

M1 - 004673

ER -

ID: 260749060