Site-directed genotype screening for elimination of antinutritional saponins in quinoa seeds identifies TSARL1 as a master controller of saponin biosynthesis selectively in seeds
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Site-directed genotype screening for elimination of antinutritional saponins in quinoa seeds identifies TSARL1 as a master controller of saponin biosynthesis selectively in seeds. / Trinh, Mai Duy Luu; Visintainer, Davide; Günther, Jan; Østerberg, Jeppe Thulin; Rodrigues da Fonseca, Rute Andreia; Fondevilla, Sara; Moog, Max William; Luo, Guangbin; Nørrevang, Anton F.; Crocoll, Christoph; Nielsen, Philip V.; Jacobsen, Sven-Erik; Wendt, Toni; Bak, Søren; López-Marqués, Rosa Laura; Palmgren, Michael.
In: Plant Biotechnology Journal, 2024.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Site-directed genotype screening for elimination of antinutritional saponins in quinoa seeds identifies TSARL1 as a master controller of saponin biosynthesis selectively in seeds
AU - Trinh, Mai Duy Luu
AU - Visintainer, Davide
AU - Günther, Jan
AU - Østerberg, Jeppe Thulin
AU - Rodrigues da Fonseca, Rute Andreia
AU - Fondevilla, Sara
AU - Moog, Max William
AU - Luo, Guangbin
AU - Nørrevang, Anton F.
AU - Crocoll, Christoph
AU - Nielsen, Philip V.
AU - Jacobsen, Sven-Erik
AU - Wendt, Toni
AU - Bak, Søren
AU - López-Marqués, Rosa Laura
AU - Palmgren, Michael
PY - 2024
Y1 - 2024
N2 - Climate change may result in a drier climate and increased salinization, threatening agricultural productivity worldwide. Quinoa (Chenopodium quinoa) produces highly nutritious seeds and tolerates abiotic stresses such as drought and high salinity, making it a promising future food source. However, the presence of antinutritional saponins in their seeds is an undesirable trait. We mapped genes controlling seed saponin content to a genomic region that includes TSARL1. We isolated desired genetic variation in this gene by producing a large mutant library of a commercial quinoa cultivar and screening the library for specific nucleotide substitutions using droplet digital PCR. We were able to rapidly isolate two independent tsarl1 mutants, which retained saponins in the leaves and roots for defence, but saponins were undetectable in the seed coat. We further could show that TSARL1 specifically controls seed saponin biosynthesis in the committed step after 2,3-oxidosqualene. Our work provides new important knowledge on the function of TSARL1 and represents a breakthrough for quinoa breeding.
AB - Climate change may result in a drier climate and increased salinization, threatening agricultural productivity worldwide. Quinoa (Chenopodium quinoa) produces highly nutritious seeds and tolerates abiotic stresses such as drought and high salinity, making it a promising future food source. However, the presence of antinutritional saponins in their seeds is an undesirable trait. We mapped genes controlling seed saponin content to a genomic region that includes TSARL1. We isolated desired genetic variation in this gene by producing a large mutant library of a commercial quinoa cultivar and screening the library for specific nucleotide substitutions using droplet digital PCR. We were able to rapidly isolate two independent tsarl1 mutants, which retained saponins in the leaves and roots for defence, but saponins were undetectable in the seed coat. We further could show that TSARL1 specifically controls seed saponin biosynthesis in the committed step after 2,3-oxidosqualene. Our work provides new important knowledge on the function of TSARL1 and represents a breakthrough for quinoa breeding.
KW - Faculty of Science
KW - Chenopodium quinoa
KW - Genome-wide association analysis
KW - Quinoa
KW - Saponin biosynthesis
KW - Site-directed genotype screening
U2 - 10.1111/pbi.14340
DO - 10.1111/pbi.14340
M3 - Journal article
JO - Plant Biotechnology Journal
JF - Plant Biotechnology Journal
SN - 1467-7644
ER -
ID: 388871913