Saliva and plasma TIMP-1 in patients with colorectal cancer: a prospective study
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Saliva and plasma TIMP-1 in patients with colorectal cancer : a prospective study. / Holten-Andersen, Lars; Christensen, Ib Jarle; Jensen, Siri Beier; Reibel, Jesper; Laurberg, Søren; Nauntofte, Birgitte; Brünner, Nils; Nielsen, Hans Jørgen.
In: Scandinavian Journal of Gastroenterology, Vol. 47, No. 10, 2012, p. 1234-1241.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Saliva and plasma TIMP-1 in patients with colorectal cancer
T2 - a prospective study
AU - Holten-Andersen, Lars
AU - Christensen, Ib Jarle
AU - Jensen, Siri Beier
AU - Reibel, Jesper
AU - Laurberg, Søren
AU - Nauntofte, Birgitte
AU - Brünner, Nils
AU - Nielsen, Hans Jørgen
PY - 2012
Y1 - 2012
N2 - Background and aims. A prospective cross-sectional study was designed to test if total levels of TIMP-1 in saliva and plasma correlated with the diagnosis of colorectal cancer (CRC) in a population with symptoms consistent with this disease. Materials and methods. Stimulated whole saliva and blood samples were collected from 161 individuals referred to colonoscopy with symptoms associated with CRC. The results of the examination, as well as previous and/or current other diseases were recorded. In a blinded study, the authors used an in-house TIMP-1 ELISA previously validated for use in saliva and plasma to determine total levels of TIMP-1. Results. Fifty-six of the patients (35%) were diagnosed with CRC. Plasma TIMP-1 levels were significantly elevated in CRC patients compared with patients with other, non-malignant diseases and individuals without disease. Significant differences in saliva TIMP-1 levels between CRC patients and individuals without CRC could not be demonstrated. In addition, no correlation was found between levels of TIMP-1 in plasma and saliva. Conclusion. Total levels of TIMP-1 in saliva do not reflect the presence of CRC, and TIMP-1 saliva measurements thus cannot substitute plasma TIMP-1 measurements in detection of CRC.
AB - Background and aims. A prospective cross-sectional study was designed to test if total levels of TIMP-1 in saliva and plasma correlated with the diagnosis of colorectal cancer (CRC) in a population with symptoms consistent with this disease. Materials and methods. Stimulated whole saliva and blood samples were collected from 161 individuals referred to colonoscopy with symptoms associated with CRC. The results of the examination, as well as previous and/or current other diseases were recorded. In a blinded study, the authors used an in-house TIMP-1 ELISA previously validated for use in saliva and plasma to determine total levels of TIMP-1. Results. Fifty-six of the patients (35%) were diagnosed with CRC. Plasma TIMP-1 levels were significantly elevated in CRC patients compared with patients with other, non-malignant diseases and individuals without disease. Significant differences in saliva TIMP-1 levels between CRC patients and individuals without CRC could not be demonstrated. In addition, no correlation was found between levels of TIMP-1 in plasma and saliva. Conclusion. Total levels of TIMP-1 in saliva do not reflect the presence of CRC, and TIMP-1 saliva measurements thus cannot substitute plasma TIMP-1 measurements in detection of CRC.
KW - Former LIFE faculty
KW - colorectal cancer
KW - early detection
KW - oral-based diagnostics
KW - saliva
KW - TIMP-1
U2 - 10.3109/00365521.2012.711855
DO - 10.3109/00365521.2012.711855
M3 - Journal article
C2 - 22871105
VL - 47
SP - 1234
EP - 1241
JO - Scandinavian Journal of Gastroenterology
JF - Scandinavian Journal of Gastroenterology
SN - 0036-5521
IS - 10
ER -
ID: 40437713