Ripples and the formation of anisotropic lipid domains: Imaging two-component supported double bilayers by atomic force microscopy

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

Ripples and the formation of anisotropic lipid domains : Imaging two-component supported double bilayers by atomic force microscopy. / Leidy, Chad; Kaasgaard, Thomas; Crowe, John H.; Mouritsen, Ole G.; Jørgensen, Kent.

In: Biophysical Journal, Vol. 83, No. 5, 01.11.2002, p. 2625-2633.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Leidy, C, Kaasgaard, T, Crowe, JH, Mouritsen, OG & Jørgensen, K 2002, 'Ripples and the formation of anisotropic lipid domains: Imaging two-component supported double bilayers by atomic force microscopy', Biophysical Journal, vol. 83, no. 5, pp. 2625-2633. https://doi.org/10.1016/S0006-3495(02)75273-9

APA

Leidy, C., Kaasgaard, T., Crowe, J. H., Mouritsen, O. G., & Jørgensen, K. (2002). Ripples and the formation of anisotropic lipid domains: Imaging two-component supported double bilayers by atomic force microscopy. Biophysical Journal, 83(5), 2625-2633. https://doi.org/10.1016/S0006-3495(02)75273-9

Vancouver

Leidy C, Kaasgaard T, Crowe JH, Mouritsen OG, Jørgensen K. Ripples and the formation of anisotropic lipid domains: Imaging two-component supported double bilayers by atomic force microscopy. Biophysical Journal. 2002 Nov 1;83(5):2625-2633. https://doi.org/10.1016/S0006-3495(02)75273-9

Author

Leidy, Chad ; Kaasgaard, Thomas ; Crowe, John H. ; Mouritsen, Ole G. ; Jørgensen, Kent. / Ripples and the formation of anisotropic lipid domains : Imaging two-component supported double bilayers by atomic force microscopy. In: Biophysical Journal. 2002 ; Vol. 83, No. 5. pp. 2625-2633.

Bibtex

@article{cd77749460f44e0093f3b09d0232fc70,
title = "Ripples and the formation of anisotropic lipid domains: Imaging two-component supported double bilayers by atomic force microscopy",
abstract = "Direct visualization of the fluid-phase/ordered-phase domain structure in mica-supported bilayers composed of 1,2-dimyristoyl-sn-glycero- 3-phosphocholine/1,2-distearoyl-sn-glycero-3-phosphocholine mixtures is performed with atomic force microscopy. The system studied is a double bilayer supported on a mica surface in which the top bilayer (which is not in direct contact with the mica) is visualized as a function of temperature. Because the top bilayer is not as restricted by the interactions with the surface as single supported bilayers, its behavior is more similar to a free-standing bilayer. Intriguing straight-edged anisotropic fluid-phase domains were observed in the fluid-phase/ordered-phase coexistence temperature range, which resemble the fluid-phase/ordered-phase domain patterns observed in giant unilamellar vesicles composed of such phospholipid mixtures. With the high resolution provided by atomic force microscopy, we investigated the origin of these anisotropic lipid domain patterns, and found that ripple phase formation is directly responsible for the anisotropic nature of these domains. The nucleation and growth of fluid-phase domains are found to be directed by the presence of ripples. In particular, the fluid-phase domains elongate parallel to the ripples. The results show that ripple phase formation may have implications for domain formation in biological systems.",
author = "Chad Leidy and Thomas Kaasgaard and Crowe, {John H.} and Mouritsen, {Ole G.} and Kent J{\o}rgensen",
year = "2002",
month = nov,
day = "1",
doi = "10.1016/S0006-3495(02)75273-9",
language = "English",
volume = "83",
pages = "2625--2633",
journal = "Biophysical Society. Annual Meeting. Abstracts",
issn = "0523-6800",
publisher = "Biophysical Society",
number = "5",

}

RIS

TY - JOUR

T1 - Ripples and the formation of anisotropic lipid domains

T2 - Imaging two-component supported double bilayers by atomic force microscopy

AU - Leidy, Chad

AU - Kaasgaard, Thomas

AU - Crowe, John H.

AU - Mouritsen, Ole G.

AU - Jørgensen, Kent

PY - 2002/11/1

Y1 - 2002/11/1

N2 - Direct visualization of the fluid-phase/ordered-phase domain structure in mica-supported bilayers composed of 1,2-dimyristoyl-sn-glycero- 3-phosphocholine/1,2-distearoyl-sn-glycero-3-phosphocholine mixtures is performed with atomic force microscopy. The system studied is a double bilayer supported on a mica surface in which the top bilayer (which is not in direct contact with the mica) is visualized as a function of temperature. Because the top bilayer is not as restricted by the interactions with the surface as single supported bilayers, its behavior is more similar to a free-standing bilayer. Intriguing straight-edged anisotropic fluid-phase domains were observed in the fluid-phase/ordered-phase coexistence temperature range, which resemble the fluid-phase/ordered-phase domain patterns observed in giant unilamellar vesicles composed of such phospholipid mixtures. With the high resolution provided by atomic force microscopy, we investigated the origin of these anisotropic lipid domain patterns, and found that ripple phase formation is directly responsible for the anisotropic nature of these domains. The nucleation and growth of fluid-phase domains are found to be directed by the presence of ripples. In particular, the fluid-phase domains elongate parallel to the ripples. The results show that ripple phase formation may have implications for domain formation in biological systems.

AB - Direct visualization of the fluid-phase/ordered-phase domain structure in mica-supported bilayers composed of 1,2-dimyristoyl-sn-glycero- 3-phosphocholine/1,2-distearoyl-sn-glycero-3-phosphocholine mixtures is performed with atomic force microscopy. The system studied is a double bilayer supported on a mica surface in which the top bilayer (which is not in direct contact with the mica) is visualized as a function of temperature. Because the top bilayer is not as restricted by the interactions with the surface as single supported bilayers, its behavior is more similar to a free-standing bilayer. Intriguing straight-edged anisotropic fluid-phase domains were observed in the fluid-phase/ordered-phase coexistence temperature range, which resemble the fluid-phase/ordered-phase domain patterns observed in giant unilamellar vesicles composed of such phospholipid mixtures. With the high resolution provided by atomic force microscopy, we investigated the origin of these anisotropic lipid domain patterns, and found that ripple phase formation is directly responsible for the anisotropic nature of these domains. The nucleation and growth of fluid-phase domains are found to be directed by the presence of ripples. In particular, the fluid-phase domains elongate parallel to the ripples. The results show that ripple phase formation may have implications for domain formation in biological systems.

UR - http://www.scopus.com/inward/record.url?scp=0036840109&partnerID=8YFLogxK

U2 - 10.1016/S0006-3495(02)75273-9

DO - 10.1016/S0006-3495(02)75273-9

M3 - Journal article

C2 - 12414696

AN - SCOPUS:0036840109

VL - 83

SP - 2625

EP - 2633

JO - Biophysical Society. Annual Meeting. Abstracts

JF - Biophysical Society. Annual Meeting. Abstracts

SN - 0523-6800

IS - 5

ER -

ID: 230987139