Replication Protein Rep Provides Selective Advantage to Viruses in the Presence of CRISPR-Cas Immunity

Research output: Contribution to journal › Journal article › Research › peer-review

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Replication Protein Rep Provides Selective Advantage to Viruses in the Presence of CRISPR-Cas Immunity. / Zhang, Weijia; Bhoobalan-Chitty, Yuvaraj; Zhai, Xichuan; Hui, Yan; Hansen, Lars Hestbjerg; Deng, Ling; Peng, Xu.

In: CRISPR Journal, Vol. 6, No. 1, 2023, p. 32-42.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Zhang, W, Bhoobalan-Chitty, Y, Zhai, X, Hui, Y, Hansen, LH, Deng, L & Peng, X 2023, 'Replication Protein Rep Provides Selective Advantage to Viruses in the Presence of CRISPR-Cas Immunity', CRISPR Journal, vol. 6, no. 1, pp. 32-42. https://doi.org/10.1089/crispr.2022.0037

APA

Zhang, W., Bhoobalan-Chitty, Y., Zhai, X., Hui, Y., Hansen, L. H., Deng, L., & Peng, X. (2023). Replication Protein Rep Provides Selective Advantage to Viruses in the Presence of CRISPR-Cas Immunity. CRISPR Journal, 6(1), 32-42. https://doi.org/10.1089/crispr.2022.0037

Vancouver

Zhang W, Bhoobalan-Chitty Y, Zhai X, Hui Y, Hansen LH, Deng L et al. Replication Protein Rep Provides Selective Advantage to Viruses in the Presence of CRISPR-Cas Immunity. CRISPR Journal. 2023;6(1):32-42. https://doi.org/10.1089/crispr.2022.0037

Author

Zhang, Weijia ; Bhoobalan-Chitty, Yuvaraj ; Zhai, Xichuan ; Hui, Yan ; Hansen, Lars Hestbjerg ; Deng, Ling ; Peng, Xu. / Replication Protein Rep Provides Selective Advantage to Viruses in the Presence of CRISPR-Cas Immunity. In: CRISPR Journal. 2023 ; Vol. 6, No. 1. pp. 32-42.

Bibtex

@article{6451d1980fea44379e06cfd35a38b4c9,
title = "Replication Protein Rep Provides Selective Advantage to Viruses in the Presence of CRISPR-Cas Immunity",
abstract = "Anti-Clustered regularly interspaced small palindromic repeat (CRISPR) (Acr) phages cooperate to establish a successful infection in CRISPR-containing host. We report here the selective advantage provided by a replication initiator, Rep, toward cooperative host immunosuppression by viruses encoding Acrs. A rep knockout mutant (Δgp16) of Sulfolobus islandicus rod-shaped virus 2 produced around fourfold less virus in a CRISPR-null host, suggesting that Rep is the major replication initiator. In addition to Rep-dependent replication initiation from the viral genomic termini, we detected Rep-independent replication initiation from nonterminal sites. Intriguingly, despite the presence of Acrs, lack of Rep showed a profound effect on virus propagation in a host carrying CRISPR-Cas immunity. Accordingly, the co-infecting parental virus (rep-containing) outcompeted the Δgp16 mutant much more quickly in the CRISPR-containing host than in CRISPR-null host. Despite the nonessentiality, rep is carried by all known members of Rudiviridae, which is likely an evolutionary outcome driven by the ubiquitous presence of CRISPR-Cas in Sulfolobales.",
author = "Weijia Zhang and Yuvaraj Bhoobalan-Chitty and Xichuan Zhai and Yan Hui and Hansen, {Lars Hestbjerg} and Ling Deng and Xu Peng",
year = "2023",
doi = "10.1089/crispr.2022.0037",
language = "English",
volume = "6",
pages = "32--42",
journal = "CRISPR Journal",
issn = "2573-1599",
publisher = "Mary AnnLiebert, Inc. Publishers",
number = "1",

}

RIS

TY - JOUR

T1 - Replication Protein Rep Provides Selective Advantage to Viruses in the Presence of CRISPR-Cas Immunity

AU - Zhang, Weijia

AU - Bhoobalan-Chitty, Yuvaraj

AU - Zhai, Xichuan

AU - Hui, Yan

AU - Hansen, Lars Hestbjerg

AU - Deng, Ling

AU - Peng, Xu

PY - 2023

Y1 - 2023

N2 - Anti-Clustered regularly interspaced small palindromic repeat (CRISPR) (Acr) phages cooperate to establish a successful infection in CRISPR-containing host. We report here the selective advantage provided by a replication initiator, Rep, toward cooperative host immunosuppression by viruses encoding Acrs. A rep knockout mutant (Δgp16) of Sulfolobus islandicus rod-shaped virus 2 produced around fourfold less virus in a CRISPR-null host, suggesting that Rep is the major replication initiator. In addition to Rep-dependent replication initiation from the viral genomic termini, we detected Rep-independent replication initiation from nonterminal sites. Intriguingly, despite the presence of Acrs, lack of Rep showed a profound effect on virus propagation in a host carrying CRISPR-Cas immunity. Accordingly, the co-infecting parental virus (rep-containing) outcompeted the Δgp16 mutant much more quickly in the CRISPR-containing host than in CRISPR-null host. Despite the nonessentiality, rep is carried by all known members of Rudiviridae, which is likely an evolutionary outcome driven by the ubiquitous presence of CRISPR-Cas in Sulfolobales.

AB - Anti-Clustered regularly interspaced small palindromic repeat (CRISPR) (Acr) phages cooperate to establish a successful infection in CRISPR-containing host. We report here the selective advantage provided by a replication initiator, Rep, toward cooperative host immunosuppression by viruses encoding Acrs. A rep knockout mutant (Δgp16) of Sulfolobus islandicus rod-shaped virus 2 produced around fourfold less virus in a CRISPR-null host, suggesting that Rep is the major replication initiator. In addition to Rep-dependent replication initiation from the viral genomic termini, we detected Rep-independent replication initiation from nonterminal sites. Intriguingly, despite the presence of Acrs, lack of Rep showed a profound effect on virus propagation in a host carrying CRISPR-Cas immunity. Accordingly, the co-infecting parental virus (rep-containing) outcompeted the Δgp16 mutant much more quickly in the CRISPR-containing host than in CRISPR-null host. Despite the nonessentiality, rep is carried by all known members of Rudiviridae, which is likely an evolutionary outcome driven by the ubiquitous presence of CRISPR-Cas in Sulfolobales.

U2 - 10.1089/crispr.2022.0037

DO - 10.1089/crispr.2022.0037

M3 - Journal article

C2 - 36576859

VL - 6

SP - 32

EP - 42

JO - CRISPR Journal

JF - CRISPR Journal

SN - 2573-1599

IS - 1

ER -

ID: 333308266