Investigation of the bacteriophage community in induced lysates of undefined mesophilic mixed-strain DL-cultures using classical and metagenomic approaches

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

Investigation of the bacteriophage community in induced lysates of undefined mesophilic mixed-strain DL-cultures using classical and metagenomic approaches. / Muhammed, Musemma Kedir; Olsen, Mette L.; Kot, Witold Piotr; Neve, Horst; Castro Mejia, Josue Leonardo; Janzen, Thomas; Hansen, Lars H.; Nielsen, Dennis Sandris; Sørensen, Søren Johannes; Heller, Knut J.; Vogensen, Finn Kvist.

In: International Journal of Food Microbiology, Vol. 272, 2018, p. 61-72.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Muhammed, MK, Olsen, ML, Kot, WP, Neve, H, Castro Mejia, JL, Janzen, T, Hansen, LH, Nielsen, DS, Sørensen, SJ, Heller, KJ & Vogensen, FK 2018, 'Investigation of the bacteriophage community in induced lysates of undefined mesophilic mixed-strain DL-cultures using classical and metagenomic approaches', International Journal of Food Microbiology, vol. 272, pp. 61-72. https://doi.org/10.1016/j.ijfoodmicro.2018.02.012

APA

Muhammed, M. K., Olsen, M. L., Kot, W. P., Neve, H., Castro Mejia, J. L., Janzen, T., Hansen, L. H., Nielsen, D. S., Sørensen, S. J., Heller, K. J., & Vogensen, F. K. (2018). Investigation of the bacteriophage community in induced lysates of undefined mesophilic mixed-strain DL-cultures using classical and metagenomic approaches. International Journal of Food Microbiology, 272, 61-72. https://doi.org/10.1016/j.ijfoodmicro.2018.02.012

Vancouver

Muhammed MK, Olsen ML, Kot WP, Neve H, Castro Mejia JL, Janzen T et al. Investigation of the bacteriophage community in induced lysates of undefined mesophilic mixed-strain DL-cultures using classical and metagenomic approaches. International Journal of Food Microbiology. 2018;272:61-72. https://doi.org/10.1016/j.ijfoodmicro.2018.02.012

Author

Muhammed, Musemma Kedir ; Olsen, Mette L. ; Kot, Witold Piotr ; Neve, Horst ; Castro Mejia, Josue Leonardo ; Janzen, Thomas ; Hansen, Lars H. ; Nielsen, Dennis Sandris ; Sørensen, Søren Johannes ; Heller, Knut J. ; Vogensen, Finn Kvist. / Investigation of the bacteriophage community in induced lysates of undefined mesophilic mixed-strain DL-cultures using classical and metagenomic approaches. In: International Journal of Food Microbiology. 2018 ; Vol. 272. pp. 61-72.

Bibtex

@article{fc0a1e2beaa24db1b1597a280314716b,

title = "Investigation of the bacteriophage community in induced lysates of undefined mesophilic mixed-strain DL-cultures using classical and metagenomic approaches",

abstract = "To investigate the notion that starter cultures can be a reservoir of bacteriophages (phages) in the dairy environment, strains of three DL-starters (undefined mesophilic mixed-strain starters containing Lactococcus lactis subsp. lactis biovar. diacetylactis and Leuconostoc species) were selected and induced by mitomycin C, and the whole starters were induced spontaneously as well as by mitomycin C. Frequency of induction of 17%, 26% and 12% was estimated among the isolates of the three starters, with majority of the induced phages mostly showing morphological similarity to known P335 phages, and with a fraction of them showing atypical features. Sequences of P335 quasi-species phages were found to be the most frequent entities in almost all metaviromes derived from the induced lysates. However, sequences of Sk1virus phages (previously 936 phages) were emerged as the predominant entities following spontaneous induction of one of the starters, suggesting a phage-carrier state. Sequences of other phages such as 949, 1706, C2virus (previously c2 phages) and Leuconostoc species could also be observed but with a lower relative frequency. Taken together, the majority of the P335 quasi-species phages could represent the induced viral community of the starters and the remaining phage groups mainly represent the background ambient viral community.",

author = "Muhammed, {Musemma Kedir} and Olsen, {Mette L.} and Kot, {Witold Piotr} and Horst Neve and {Castro Mejia}, {Josue Leonardo} and Thomas Janzen and Hansen, {Lars H.} and Nielsen, {Dennis Sandris} and S{\o}rensen, {S{\o}ren Johannes} and Heller, {Knut J.} and Vogensen, {Finn Kvist}",

year = "2018",

doi = "10.1016/j.ijfoodmicro.2018.02.012",

language = "English",

volume = "272",

pages = "61--72",

journal = "International Journal of Food Microbiology",

issn = "0168-1605",

publisher = "Elsevier",

}

RIS

TY - JOUR

T1 - Investigation of the bacteriophage community in induced lysates of undefined mesophilic mixed-strain DL-cultures using classical and metagenomic approaches

AU - Muhammed, Musemma Kedir

AU - Olsen, Mette L.

AU - Kot, Witold Piotr

AU - Neve, Horst

AU - Castro Mejia, Josue Leonardo

AU - Janzen, Thomas

AU - Hansen, Lars H.

AU - Nielsen, Dennis Sandris

AU - Sørensen, Søren Johannes

AU - Heller, Knut J.

AU - Vogensen, Finn Kvist

PY - 2018

Y1 - 2018

N2 - To investigate the notion that starter cultures can be a reservoir of bacteriophages (phages) in the dairy environment, strains of three DL-starters (undefined mesophilic mixed-strain starters containing Lactococcus lactis subsp. lactis biovar. diacetylactis and Leuconostoc species) were selected and induced by mitomycin C, and the whole starters were induced spontaneously as well as by mitomycin C. Frequency of induction of 17%, 26% and 12% was estimated among the isolates of the three starters, with majority of the induced phages mostly showing morphological similarity to known P335 phages, and with a fraction of them showing atypical features. Sequences of P335 quasi-species phages were found to be the most frequent entities in almost all metaviromes derived from the induced lysates. However, sequences of Sk1virus phages (previously 936 phages) were emerged as the predominant entities following spontaneous induction of one of the starters, suggesting a phage-carrier state. Sequences of other phages such as 949, 1706, C2virus (previously c2 phages) and Leuconostoc species could also be observed but with a lower relative frequency. Taken together, the majority of the P335 quasi-species phages could represent the induced viral community of the starters and the remaining phage groups mainly represent the background ambient viral community.

AB - To investigate the notion that starter cultures can be a reservoir of bacteriophages (phages) in the dairy environment, strains of three DL-starters (undefined mesophilic mixed-strain starters containing Lactococcus lactis subsp. lactis biovar. diacetylactis and Leuconostoc species) were selected and induced by mitomycin C, and the whole starters were induced spontaneously as well as by mitomycin C. Frequency of induction of 17%, 26% and 12% was estimated among the isolates of the three starters, with majority of the induced phages mostly showing morphological similarity to known P335 phages, and with a fraction of them showing atypical features. Sequences of P335 quasi-species phages were found to be the most frequent entities in almost all metaviromes derived from the induced lysates. However, sequences of Sk1virus phages (previously 936 phages) were emerged as the predominant entities following spontaneous induction of one of the starters, suggesting a phage-carrier state. Sequences of other phages such as 949, 1706, C2virus (previously c2 phages) and Leuconostoc species could also be observed but with a lower relative frequency. Taken together, the majority of the P335 quasi-species phages could represent the induced viral community of the starters and the remaining phage groups mainly represent the background ambient viral community.

U2 - 10.1016/j.ijfoodmicro.2018.02.012

DO - 10.1016/j.ijfoodmicro.2018.02.012

M3 - Journal article

C2 - 29529425

VL - 272

SP - 61

EP - 72

JO - International Journal of Food Microbiology

JF - International Journal of Food Microbiology

SN - 0168-1605

ER -

ID: 195903858

Department of Food Science