Intrinsic reaction-cycle time scale of Na+,K+-ATPase manifests itself in the lipid-protein interactions of nonequilibrium membranes

Research output: Contribution to journal › Journal article › Research › peer-review

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Intrinsic reaction-cycle time scale of Na+,K+-ATPase manifests itself in the lipid-protein interactions of nonequilibrium membranes. / Bouvrais, Hélène; Cornelius, Flemming; Ipsen, John H.; Mouritsen, Ole G.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 109, No. 45, 2012, p. 18442-18446.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Bouvrais, H, Cornelius, F, Ipsen, JH & Mouritsen, OG 2012, 'Intrinsic reaction-cycle time scale of Na+,K+-ATPase manifests itself in the lipid-protein interactions of nonequilibrium membranes', Proceedings of the National Academy of Sciences of the United States of America, vol. 109, no. 45, pp. 18442-18446. https://doi.org/10.1073/pnas.1209909109

APA

Bouvrais, H., Cornelius, F., Ipsen, J. H., & Mouritsen, O. G. (2012). Intrinsic reaction-cycle time scale of Na+,K+-ATPase manifests itself in the lipid-protein interactions of nonequilibrium membranes. Proceedings of the National Academy of Sciences of the United States of America, 109(45), 18442-18446. https://doi.org/10.1073/pnas.1209909109

Vancouver

Bouvrais H, Cornelius F, Ipsen JH, Mouritsen OG. Intrinsic reaction-cycle time scale of Na+,K+-ATPase manifests itself in the lipid-protein interactions of nonequilibrium membranes. Proceedings of the National Academy of Sciences of the United States of America. 2012;109(45):18442-18446. https://doi.org/10.1073/pnas.1209909109

Author

Bouvrais, Hélène ; Cornelius, Flemming ; Ipsen, John H. ; Mouritsen, Ole G. / Intrinsic reaction-cycle time scale of Na+,K+-ATPase manifests itself in the lipid-protein interactions of nonequilibrium membranes. In: Proceedings of the National Academy of Sciences of the United States of America. 2012 ; Vol. 109, No. 45. pp. 18442-18446.

Bibtex

@article{ac1d3081903f4f879a651fbbd69ca05f,
title = "Intrinsic reaction-cycle time scale of Na+,K+-ATPase manifests itself in the lipid-protein interactions of nonequilibrium membranes",
abstract = "Interaction between integral membrane proteins and the lipid-bilayer component of biological membranes is expected to mutually infl uence the proteins and the membrane. We present quantitative evidence of a manifestation of the lipid-protein interactions in liposomal membranes, reconstituted with actively pumping Na+,K+-ATPase, in terms of nonequilibrium shape fluctuations that contain a relaxation time, τ, which is robust and independent of the speci fi c fl uctuation modes of the membrane. In the case of pumping Na+-ions, analysis of the flicker-noise temporal correlation spectrum of the liposomes leads to τ ≃ 0.5 s, comparing favorably with an intrinsic reaction-cycle time of about 0.4 s from enzymology.",
keywords = "Membrane dynamics, Sodium pump reconstituted in giant vesicles, Vesicle fluctuation analysis",
author = "H{\'e}l{\`e}ne Bouvrais and Flemming Cornelius and Ipsen, {John H.} and Mouritsen, {Ole G.}",
year = "2012",
doi = "10.1073/pnas.1209909109",
language = "English",
volume = "109",
pages = "18442--18446",
journal = "Proceedings of the National Academy of Sciences of the United States of America",
issn = "0027-8424",
publisher = "The National Academy of Sciences of the United States of America",
number = "45",

}

RIS

TY - JOUR

T1 - Intrinsic reaction-cycle time scale of Na+,K+-ATPase manifests itself in the lipid-protein interactions of nonequilibrium membranes

AU - Bouvrais, Hélène

AU - Cornelius, Flemming

AU - Ipsen, John H.

AU - Mouritsen, Ole G.

PY - 2012

Y1 - 2012

N2 - Interaction between integral membrane proteins and the lipid-bilayer component of biological membranes is expected to mutually infl uence the proteins and the membrane. We present quantitative evidence of a manifestation of the lipid-protein interactions in liposomal membranes, reconstituted with actively pumping Na+,K+-ATPase, in terms of nonequilibrium shape fluctuations that contain a relaxation time, τ, which is robust and independent of the speci fi c fl uctuation modes of the membrane. In the case of pumping Na+-ions, analysis of the flicker-noise temporal correlation spectrum of the liposomes leads to τ ≃ 0.5 s, comparing favorably with an intrinsic reaction-cycle time of about 0.4 s from enzymology.

AB - Interaction between integral membrane proteins and the lipid-bilayer component of biological membranes is expected to mutually infl uence the proteins and the membrane. We present quantitative evidence of a manifestation of the lipid-protein interactions in liposomal membranes, reconstituted with actively pumping Na+,K+-ATPase, in terms of nonequilibrium shape fluctuations that contain a relaxation time, τ, which is robust and independent of the speci fi c fl uctuation modes of the membrane. In the case of pumping Na+-ions, analysis of the flicker-noise temporal correlation spectrum of the liposomes leads to τ ≃ 0.5 s, comparing favorably with an intrinsic reaction-cycle time of about 0.4 s from enzymology.

KW - Membrane dynamics

KW - Sodium pump reconstituted in giant vesicles

KW - Vesicle fluctuation analysis

U2 - 10.1073/pnas.1209909109

DO - 10.1073/pnas.1209909109

M3 - Journal article

C2 - 23093677

AN - SCOPUS:84868547929

VL - 109

SP - 18442

EP - 18446

JO - Proceedings of the National Academy of Sciences of the United States of America

JF - Proceedings of the National Academy of Sciences of the United States of America

SN - 0027-8424

IS - 45

ER -

ID: 230975063