Inhibition of lpmos by fermented persimmon juice
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Inhibition of lpmos by fermented persimmon juice. / Tokin, Radina; Ipsen, Johan Ørskov; Poojary, Mahesha M.; Jensen, Poul Erik; Olsson, Lisbeth; Johansen, Katja Salomon.
In: Biomolecules, Vol. 11, No. 12, 1890, 2021.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Inhibition of lpmos by fermented persimmon juice
AU - Tokin, Radina
AU - Ipsen, Johan Ørskov
AU - Poojary, Mahesha M.
AU - Jensen, Poul Erik
AU - Olsson, Lisbeth
AU - Johansen, Katja Salomon
N1 - Funding Information: Funding: This research was funded by The Novo Nordisk Foundation (Grant number NNF17SA0027704 to Katja Salomon Johansen). Publisher Copyright: © 2021 by the authors. Licensee MDPI, Basel, Switzerland.
PY - 2021
Y1 - 2021
N2 - Fermented persimmon juice, Kakishibu, has traditionally been used for wood and paper protection. This protective effect stems at least partially from inhibition of microbial cellulose degrading enzymes. The inhibitory effect of Kakishibu on lytic polysaccharide monooxygenases (LPMOs) and on a cocktail of cellulose hydrolases was studied, using three different cellulosic substrates. Dose dependent inhibition of LPMO activity by a commercial Kakishibu product was assessed for the well-characterized LPMO from Thermoascus aurantiacus TaAA9A, and the inhibitory effect was confirmed on five additional microbial LPMOs. The model tannin compound, tannic acid exhibited a similar inhibitory effect on TaAA9A as Kakishibu. It was further shown that both polyethylene glycol and tannase can alleviate the inhibitory effect of Kakishibu and tannic acid, indicating a likely mechanism of inhibition caused by unspecific tannin–protein interactions.
AB - Fermented persimmon juice, Kakishibu, has traditionally been used for wood and paper protection. This protective effect stems at least partially from inhibition of microbial cellulose degrading enzymes. The inhibitory effect of Kakishibu on lytic polysaccharide monooxygenases (LPMOs) and on a cocktail of cellulose hydrolases was studied, using three different cellulosic substrates. Dose dependent inhibition of LPMO activity by a commercial Kakishibu product was assessed for the well-characterized LPMO from Thermoascus aurantiacus TaAA9A, and the inhibitory effect was confirmed on five additional microbial LPMOs. The model tannin compound, tannic acid exhibited a similar inhibitory effect on TaAA9A as Kakishibu. It was further shown that both polyethylene glycol and tannase can alleviate the inhibitory effect of Kakishibu and tannic acid, indicating a likely mechanism of inhibition caused by unspecific tannin–protein interactions.
KW - Cellulase
KW - Inhibition
KW - Kakishibu
KW - LPMO
KW - PEG
KW - Tannins
U2 - 10.3390/biom11121890
DO - 10.3390/biom11121890
M3 - Journal article
C2 - 34944533
AN - SCOPUS:85121154854
VL - 11
JO - Biomolecules
JF - Biomolecules
SN - 2218-273X
IS - 12
M1 - 1890
ER -
ID: 288118332