In vivo assembly of DNA-fragments in the moss, Physcomitrella patens

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

In vivo assembly of DNA-fragments in the moss, Physcomitrella patens. / King, Brian Christopher; Vavitsas, Konstantinos; Binti Khairul Ikram, Nur Kusaira; Schrøder, Josephine; Scharff, Lars; Hamberger, Björn Robert; Jensen, Poul Erik; Simonsen, Henrik Toft.

In: Scientific Reports, Vol. 6, 25030, 2016.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

King, BC, Vavitsas, K, Binti Khairul Ikram, NK, Schrøder, J, Scharff, L, Hamberger, BR, Jensen, PE & Simonsen, HT 2016, 'In vivo assembly of DNA-fragments in the moss, Physcomitrella patens', Scientific Reports, vol. 6, 25030. https://doi.org/10.1038/srep25030

APA

King, B. C., Vavitsas, K., Binti Khairul Ikram, N. K., Schrøder, J., Scharff, L., Hamberger, B. R., Jensen, P. E., & Simonsen, H. T. (2016). In vivo assembly of DNA-fragments in the moss, Physcomitrella patens. Scientific Reports, 6, [25030]. https://doi.org/10.1038/srep25030

Vancouver

King BC, Vavitsas K, Binti Khairul Ikram NK, Schrøder J, Scharff L, Hamberger BR et al. In vivo assembly of DNA-fragments in the moss, Physcomitrella patens. Scientific Reports. 2016;6. 25030. https://doi.org/10.1038/srep25030

Author

King, Brian Christopher ; Vavitsas, Konstantinos ; Binti Khairul Ikram, Nur Kusaira ; Schrøder, Josephine ; Scharff, Lars ; Hamberger, Björn Robert ; Jensen, Poul Erik ; Simonsen, Henrik Toft. / In vivo assembly of DNA-fragments in the moss, Physcomitrella patens. In: Scientific Reports. 2016 ; Vol. 6.

Bibtex

@article{d0d72cec14584fc3ae68c95a74114be3,
title = "In vivo assembly of DNA-fragments in the moss, Physcomitrella patens",
abstract = "Direct assembly of multiple linear DNA fragments via homologous recombination, a phenomenon known as in vivo assembly or transformation associated recombination, is used in biotechnology to assemble DNA constructs ranging in size from a few kilobases to full synthetic microbial genomes. It has also enabled the complete replacement of eukaryotic chromosomes with heterologous DNA. The moss Physcomitrella patens, a non-vascular and spore producing land plant (Bryophyte), has a well-established capacity for homologous recombination. Here, we demonstrate the in vivo assembly of multiple DNA fragments in P. patens with three examples of effective genome editing: we (i) efficiently deleted a genomic locus for diterpenoid metabolism yielding a biosynthetic knockout, (ii) introduced a salt inducible promoter, and (iii) re-routed endogenous metabolism into the formation of amorphadiene, a precursor of high-value therapeutics. These proof-of-principle experiments pave the way for more complex and increasingly flexible approaches for large-scale metabolic engineering in plant biotechnology.",
author = "King, {Brian Christopher} and Konstantinos Vavitsas and {Binti Khairul Ikram}, {Nur Kusaira} and Josephine Schr{\o}der and Lars Scharff and Hamberger, {Bj{\"o}rn Robert} and Jensen, {Poul Erik} and Simonsen, {Henrik Toft}",
year = "2016",
doi = "10.1038/srep25030",
language = "English",
volume = "6",
journal = "Scientific Reports",
issn = "2045-2322",
publisher = "nature publishing group",

}

RIS

TY - JOUR

T1 - In vivo assembly of DNA-fragments in the moss, Physcomitrella patens

AU - King, Brian Christopher

AU - Vavitsas, Konstantinos

AU - Binti Khairul Ikram, Nur Kusaira

AU - Schrøder, Josephine

AU - Scharff, Lars

AU - Hamberger, Björn Robert

AU - Jensen, Poul Erik

AU - Simonsen, Henrik Toft

PY - 2016

Y1 - 2016

N2 - Direct assembly of multiple linear DNA fragments via homologous recombination, a phenomenon known as in vivo assembly or transformation associated recombination, is used in biotechnology to assemble DNA constructs ranging in size from a few kilobases to full synthetic microbial genomes. It has also enabled the complete replacement of eukaryotic chromosomes with heterologous DNA. The moss Physcomitrella patens, a non-vascular and spore producing land plant (Bryophyte), has a well-established capacity for homologous recombination. Here, we demonstrate the in vivo assembly of multiple DNA fragments in P. patens with three examples of effective genome editing: we (i) efficiently deleted a genomic locus for diterpenoid metabolism yielding a biosynthetic knockout, (ii) introduced a salt inducible promoter, and (iii) re-routed endogenous metabolism into the formation of amorphadiene, a precursor of high-value therapeutics. These proof-of-principle experiments pave the way for more complex and increasingly flexible approaches for large-scale metabolic engineering in plant biotechnology.

AB - Direct assembly of multiple linear DNA fragments via homologous recombination, a phenomenon known as in vivo assembly or transformation associated recombination, is used in biotechnology to assemble DNA constructs ranging in size from a few kilobases to full synthetic microbial genomes. It has also enabled the complete replacement of eukaryotic chromosomes with heterologous DNA. The moss Physcomitrella patens, a non-vascular and spore producing land plant (Bryophyte), has a well-established capacity for homologous recombination. Here, we demonstrate the in vivo assembly of multiple DNA fragments in P. patens with three examples of effective genome editing: we (i) efficiently deleted a genomic locus for diterpenoid metabolism yielding a biosynthetic knockout, (ii) introduced a salt inducible promoter, and (iii) re-routed endogenous metabolism into the formation of amorphadiene, a precursor of high-value therapeutics. These proof-of-principle experiments pave the way for more complex and increasingly flexible approaches for large-scale metabolic engineering in plant biotechnology.

U2 - 10.1038/srep25030

DO - 10.1038/srep25030

M3 - Journal article

C2 - 27126800

VL - 6

JO - Scientific Reports

JF - Scientific Reports

SN - 2045-2322

M1 - 25030

ER -

ID: 161597488