In situ atomic force microscope imaging of supported lipid bilayers

Research output: Contribution to journalJournal articleResearchpeer-review

  • Thomas Kaasgaard
  • Chad Leidy
  • John Hjort Ipsen
  • Ole G. Mouritsen
  • Kent Jørgensen

In situ AFM images of phospholipase A2 (PLA2) hydrolysis of mica-supported one- and two-component lipid bilayers are presented. For one-component DPPC bilayers an enhanced enzymatic activity is observed towards preexisting defects in the bilayer. Phase separation is observed in two-component DMPC-DSPC bilayers and a remarkable enhanced hydrolytic activity of the PLA2-enzyme for the DMPC-rich phase is seen. Furthermore, in a supported double bilayer system a characteristic ripple structure, most likely related to the formation of the Pβ'-ripple phase is observed.

Original languageEnglish
JournalSingle Molecules
Volume2
Issue number2
Pages (from-to)105-108
Number of pages4
ISSN1438-5163
DOIs
Publication statusPublished - 1 Jan 2001

ID: 236896940