Improving the Phosphoproteome Coverage for Limited Sample Amounts Using TiO2-SIMAC-HILIC (TiSH) Phosphopeptide Enrichment and Fractionation

Research output: Chapter in Book/Report/Conference proceeding › Book chapter › Research › peer-review

Standard

Improving the Phosphoproteome Coverage for Limited Sample Amounts Using TiO2-SIMAC-HILIC (TiSH) Phosphopeptide Enrichment and Fractionation. / Engholm-Keller, Kasper; Larsen, Martin Røssel.

Phospho-Proteomics: Methods and Protocols. ed. / Louise von Stechow. 2. ed. Humana Press, 2016. p. 161–177 (Methods in Molecular Biology, Vol. 1355).

Research output: Chapter in Book/Report/Conference proceeding › Book chapter › Research › peer-review

Harvard

Engholm-Keller, K & Larsen, MR 2016, Improving the Phosphoproteome Coverage for Limited Sample Amounts Using TiO2-SIMAC-HILIC (TiSH) Phosphopeptide Enrichment and Fractionation. in L von Stechow (ed.), Phospho-Proteomics: Methods and Protocols. 2. edn, Humana Press, Methods in Molecular Biology, vol. 1355, pp. 161–177.

APA

Engholm-Keller, K., & Larsen, M. R. (2016). Improving the Phosphoproteome Coverage for Limited Sample Amounts Using TiO2-SIMAC-HILIC (TiSH) Phosphopeptide Enrichment and Fractionation. In L. von Stechow (Ed.), Phospho-Proteomics: Methods and Protocols (2. ed., pp. 161–177). Humana Press. Methods in Molecular Biology Vol. 1355

Vancouver

Engholm-Keller K, Larsen MR. Improving the Phosphoproteome Coverage for Limited Sample Amounts Using TiO2-SIMAC-HILIC (TiSH) Phosphopeptide Enrichment and Fractionation. In von Stechow L, editor, Phospho-Proteomics: Methods and Protocols. 2. ed. Humana Press. 2016. p. 161–177. (Methods in Molecular Biology, Vol. 1355).

Author

Engholm-Keller, Kasper ; Larsen, Martin Røssel. / Improving the Phosphoproteome Coverage for Limited Sample Amounts Using TiO2-SIMAC-HILIC (TiSH) Phosphopeptide Enrichment and Fractionation. Phospho-Proteomics: Methods and Protocols. editor / Louise von Stechow. 2. ed. Humana Press, 2016. pp. 161–177 (Methods in Molecular Biology, Vol. 1355).

Bibtex

@inbook{b445c976c3f3485f8e2c369d46ca7874,
title = "Improving the Phosphoproteome Coverage for Limited Sample Amounts Using TiO2-SIMAC-HILIC (TiSH) Phosphopeptide Enrichment and Fractionation",
abstract = "Obtaining high phosphoproteome coverage requires specific enrichment of phosphorylated peptides from the often extremely complex peptide mixtures generated by proteolytic digestion of biological samples, as well as extensive chromatographic fractionation prior to liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis. Due to the sample loss resulting from fractionation, this procedure is mainly performed when large quantities of sample are available. To make large-scale phosphoproteomics applicable to smaller amounts of protein we have recently combined highly specific TiO2-based phosphopeptide enrichment with sequential elution from immobilized metal affinity chromatography (SIMAC) for fractionation of mono- and multi-phosphorylated peptides prior to capillary scale hydrophilic interaction liquid chromatography (HILIC) based fractionation of monophosphorylated peptides. In the following protocol we describe the procedure step by step to allow for comprehensive coverage of the phosphoproteome utilizing only a few hundred micrograms of protein.",
author = "Kasper Engholm-Keller and Larsen, {Martin R{\o}ssel}",
year = "2016",
language = "English",
isbn = "978-1-4939-3048-7 ",
series = "Methods in Molecular Biology",
publisher = "Humana Press",
pages = "161–177",
editor = "{von Stechow}, Louise",
booktitle = "Phospho-Proteomics",
address = "United States",
edition = "2.",

}

RIS

TY - CHAP

T1 - Improving the Phosphoproteome Coverage for Limited Sample Amounts Using TiO2-SIMAC-HILIC (TiSH) Phosphopeptide Enrichment and Fractionation

AU - Engholm-Keller, Kasper

AU - Larsen, Martin Røssel

PY - 2016

Y1 - 2016

N2 - Obtaining high phosphoproteome coverage requires specific enrichment of phosphorylated peptides from the often extremely complex peptide mixtures generated by proteolytic digestion of biological samples, as well as extensive chromatographic fractionation prior to liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis. Due to the sample loss resulting from fractionation, this procedure is mainly performed when large quantities of sample are available. To make large-scale phosphoproteomics applicable to smaller amounts of protein we have recently combined highly specific TiO2-based phosphopeptide enrichment with sequential elution from immobilized metal affinity chromatography (SIMAC) for fractionation of mono- and multi-phosphorylated peptides prior to capillary scale hydrophilic interaction liquid chromatography (HILIC) based fractionation of monophosphorylated peptides. In the following protocol we describe the procedure step by step to allow for comprehensive coverage of the phosphoproteome utilizing only a few hundred micrograms of protein.

AB - Obtaining high phosphoproteome coverage requires specific enrichment of phosphorylated peptides from the often extremely complex peptide mixtures generated by proteolytic digestion of biological samples, as well as extensive chromatographic fractionation prior to liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis. Due to the sample loss resulting from fractionation, this procedure is mainly performed when large quantities of sample are available. To make large-scale phosphoproteomics applicable to smaller amounts of protein we have recently combined highly specific TiO2-based phosphopeptide enrichment with sequential elution from immobilized metal affinity chromatography (SIMAC) for fractionation of mono- and multi-phosphorylated peptides prior to capillary scale hydrophilic interaction liquid chromatography (HILIC) based fractionation of monophosphorylated peptides. In the following protocol we describe the procedure step by step to allow for comprehensive coverage of the phosphoproteome utilizing only a few hundred micrograms of protein.

M3 - Book chapter

SN - 978-1-4939-3048-7

T3 - Methods in Molecular Biology

SP - 161

EP - 177

BT - Phospho-Proteomics

A2 - von Stechow, Louise

PB - Humana Press

ER -

ID: 369990538