Immunomodulatory effects of honey cannot be distinguished from endotoxin

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Immunomodulatory effects of honey cannot be distinguished from endotoxin. / Timm, Michael; Bartelt, Stine; Hansen, Erik Wind.

In: Cytokine, Vol. 42, No. 1, 2008, p. 113-20.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Timm, M, Bartelt, S & Hansen, EW 2008, 'Immunomodulatory effects of honey cannot be distinguished from endotoxin', Cytokine, vol. 42, no. 1, pp. 113-20. https://doi.org/10.1016/j.cyto.2008.01.005

APA

Timm, M., Bartelt, S., & Hansen, E. W. (2008). Immunomodulatory effects of honey cannot be distinguished from endotoxin. Cytokine, 42(1), 113-20. https://doi.org/10.1016/j.cyto.2008.01.005

Vancouver

Timm M, Bartelt S, Hansen EW. Immunomodulatory effects of honey cannot be distinguished from endotoxin. Cytokine. 2008;42(1):113-20. https://doi.org/10.1016/j.cyto.2008.01.005

Author

Timm, Michael ; Bartelt, Stine ; Hansen, Erik Wind. / Immunomodulatory effects of honey cannot be distinguished from endotoxin. In: Cytokine. 2008 ; Vol. 42, No. 1. pp. 113-20.

Bibtex

@article{1d3c3b500ac311ddbee902004c4f4f50,
title = "Immunomodulatory effects of honey cannot be distinguished from endotoxin",
abstract = "In recent years, the use of honey has re-emerged as a remedy for wound treatment. Effects of honey have been related to the presence of an unidentified component that induces release of inflammatory cytokines from monocytic cells. The present study was intended to further characterize the reported in vitro effects of honey. Our results show that natural honeys induce interleukin-6 release from Mono Mac 6 cells as well as release of reactive oxygen species from all-trans retinoic acid (ATRA) differentiated HL-60 cells. The natural honeys contained substantial amounts of endotoxin, and the responses observed in the cell based assays were similar to the responses induced by endotoxin alone. In addition, we determined that the immunomodulatory component present in the natural honeys was retained in the ultra filtrated fraction with a molecular weight greater than 20 kDa. The component was resistant to boiling and its immunomodulatory activity could be abrogated by the addition of polymyxin B. We speculate that the observed in vitro immunomodulatory effects of honey might solely be explained by the endotoxin content in the natural honeys.",
keywords = "Former Faculty of Pharmaceutical Sciences",
author = "Michael Timm and Stine Bartelt and Hansen, {Erik Wind}",
year = "2008",
doi = "10.1016/j.cyto.2008.01.005",
language = "English",
volume = "42",
pages = "113--20",
journal = "Cytokine",
issn = "1043-4666",
publisher = "Academic Press",
number = "1",

}

RIS

TY - JOUR

T1 - Immunomodulatory effects of honey cannot be distinguished from endotoxin

AU - Timm, Michael

AU - Bartelt, Stine

AU - Hansen, Erik Wind

PY - 2008

Y1 - 2008

N2 - In recent years, the use of honey has re-emerged as a remedy for wound treatment. Effects of honey have been related to the presence of an unidentified component that induces release of inflammatory cytokines from monocytic cells. The present study was intended to further characterize the reported in vitro effects of honey. Our results show that natural honeys induce interleukin-6 release from Mono Mac 6 cells as well as release of reactive oxygen species from all-trans retinoic acid (ATRA) differentiated HL-60 cells. The natural honeys contained substantial amounts of endotoxin, and the responses observed in the cell based assays were similar to the responses induced by endotoxin alone. In addition, we determined that the immunomodulatory component present in the natural honeys was retained in the ultra filtrated fraction with a molecular weight greater than 20 kDa. The component was resistant to boiling and its immunomodulatory activity could be abrogated by the addition of polymyxin B. We speculate that the observed in vitro immunomodulatory effects of honey might solely be explained by the endotoxin content in the natural honeys.

AB - In recent years, the use of honey has re-emerged as a remedy for wound treatment. Effects of honey have been related to the presence of an unidentified component that induces release of inflammatory cytokines from monocytic cells. The present study was intended to further characterize the reported in vitro effects of honey. Our results show that natural honeys induce interleukin-6 release from Mono Mac 6 cells as well as release of reactive oxygen species from all-trans retinoic acid (ATRA) differentiated HL-60 cells. The natural honeys contained substantial amounts of endotoxin, and the responses observed in the cell based assays were similar to the responses induced by endotoxin alone. In addition, we determined that the immunomodulatory component present in the natural honeys was retained in the ultra filtrated fraction with a molecular weight greater than 20 kDa. The component was resistant to boiling and its immunomodulatory activity could be abrogated by the addition of polymyxin B. We speculate that the observed in vitro immunomodulatory effects of honey might solely be explained by the endotoxin content in the natural honeys.

KW - Former Faculty of Pharmaceutical Sciences

U2 - 10.1016/j.cyto.2008.01.005

DO - 10.1016/j.cyto.2008.01.005

M3 - Journal article

C2 - 18313938

VL - 42

SP - 113

EP - 120

JO - Cytokine

JF - Cytokine

SN - 1043-4666

IS - 1

ER -

ID: 3669152