Gut microbiota in human adults with type 2 diabetes differs from non-diabetic adults

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Gut microbiota in human adults with type 2 diabetes differs from non-diabetic adults. / Larsen, Nadja; Vogensen, Finn Kvist; van der Berg, Franciscus Winfried J; Nielsen, Dennis Sandris; Andreasen, Anne Sofie; Pedersen, Bente Klarlund; Abu Al-Soud, Waleed Mohamad Abdel F; Sørensen, Søren Johannes; Hansen, Lars H.; Jakobsen, Mogens.

In: PLoS ONE, Vol. 5, No. 2, 2010, p. e9085.

Research output: Contribution to journalJournal articleResearchpeer-review

Harvard

Larsen, N, Vogensen, FK, van der Berg, FWJ, Nielsen, DS, Andreasen, AS, Pedersen, BK, Abu Al-Soud, WMAF, Sørensen, SJ, Hansen, LH & Jakobsen, M 2010, 'Gut microbiota in human adults with type 2 diabetes differs from non-diabetic adults', PLoS ONE, vol. 5, no. 2, pp. e9085. https://doi.org/10.1371/journal.pone.0009085

APA

Larsen, N., Vogensen, F. K., van der Berg, F. W. J., Nielsen, D. S., Andreasen, A. S., Pedersen, B. K., ... Jakobsen, M. (2010). Gut microbiota in human adults with type 2 diabetes differs from non-diabetic adults. PLoS ONE, 5(2), e9085. https://doi.org/10.1371/journal.pone.0009085

Vancouver

Larsen N, Vogensen FK, van der Berg FWJ, Nielsen DS, Andreasen AS, Pedersen BK et al. Gut microbiota in human adults with type 2 diabetes differs from non-diabetic adults. PLoS ONE. 2010;5(2):e9085. https://doi.org/10.1371/journal.pone.0009085

Author

Larsen, Nadja ; Vogensen, Finn Kvist ; van der Berg, Franciscus Winfried J ; Nielsen, Dennis Sandris ; Andreasen, Anne Sofie ; Pedersen, Bente Klarlund ; Abu Al-Soud, Waleed Mohamad Abdel F ; Sørensen, Søren Johannes ; Hansen, Lars H. ; Jakobsen, Mogens. / Gut microbiota in human adults with type 2 diabetes differs from non-diabetic adults. In: PLoS ONE. 2010 ; Vol. 5, No. 2. pp. e9085.

Bibtex

@article{4a01a340284511df8ed1000ea68e967b,
title = "Gut microbiota in human adults with type 2 diabetes differs from non-diabetic adults",
abstract = "BackgroundRecent evidence suggests that there is a link between metabolic diseases and bacterial populations in the gut. The aim of this study was to assess the differences between the composition of the intestinal microbiota in humans with type 2 diabetes and non-diabetic persons as control.Methods and FindingsThe study included 36 male adults with a broad range of age and body-mass indices (BMIs), among which 18 subjects were diagnosed with diabetes type 2. The fecal bacterial composition was investigated by real-time quantitative PCR (qPCR) and in a subgroup of subjects (N = 20) by tag-encoded amplicon pyrosequencing of the V4 region of the 16S rRNA gene. The proportions of phylum Firmicutes and class Clostridia were significantly reduced in the diabetic group compared to the control group (P = 0.03). Furthermore, the ratios of Bacteroidetes to Firmicutes as well as the ratios of Bacteroides-Prevotella group to C. coccoides-E. rectale group correlated positively and significantly with plasma glucose concentration (P = 0.04) but not with BMIs. Similarly, class Betaproteobacteria was highly enriched in diabetic compared to non-diabetic persons (P = 0.02) and positively correlated with plasma glucose (P = 0.04).ConclusionsThe results of this study indicate that type 2 diabetes in humans is associated with compositional changes in intestinal microbiota. The level of glucose tolerance should be considered when linking microbiota with metabolic diseases such as obesity and developing strategies to control metabolic diseases by modifying the gut microbiota.",
author = "Nadja Larsen and Vogensen, {Finn Kvist} and {van der Berg}, {Franciscus Winfried J} and Nielsen, {Dennis Sandris} and Andreasen, {Anne Sofie} and Pedersen, {Bente Klarlund} and {Abu Al-Soud}, {Waleed Mohamad Abdel F} and S{\o}rensen, {S{\o}ren Johannes} and Hansen, {Lars H.} and Mogens Jakobsen",
year = "2010",
doi = "10.1371/journal.pone.0009085",
language = "English",
volume = "5",
pages = "e9085",
journal = "P L o S One",
issn = "1932-6203",
publisher = "Public Library of Science",
number = "2",

}

RIS

TY - JOUR

T1 - Gut microbiota in human adults with type 2 diabetes differs from non-diabetic adults

AU - Larsen, Nadja

AU - Vogensen, Finn Kvist

AU - van der Berg, Franciscus Winfried J

AU - Nielsen, Dennis Sandris

AU - Andreasen, Anne Sofie

AU - Pedersen, Bente Klarlund

AU - Abu Al-Soud, Waleed Mohamad Abdel F

AU - Sørensen, Søren Johannes

AU - Hansen, Lars H.

AU - Jakobsen, Mogens

PY - 2010

Y1 - 2010

N2 - BackgroundRecent evidence suggests that there is a link between metabolic diseases and bacterial populations in the gut. The aim of this study was to assess the differences between the composition of the intestinal microbiota in humans with type 2 diabetes and non-diabetic persons as control.Methods and FindingsThe study included 36 male adults with a broad range of age and body-mass indices (BMIs), among which 18 subjects were diagnosed with diabetes type 2. The fecal bacterial composition was investigated by real-time quantitative PCR (qPCR) and in a subgroup of subjects (N = 20) by tag-encoded amplicon pyrosequencing of the V4 region of the 16S rRNA gene. The proportions of phylum Firmicutes and class Clostridia were significantly reduced in the diabetic group compared to the control group (P = 0.03). Furthermore, the ratios of Bacteroidetes to Firmicutes as well as the ratios of Bacteroides-Prevotella group to C. coccoides-E. rectale group correlated positively and significantly with plasma glucose concentration (P = 0.04) but not with BMIs. Similarly, class Betaproteobacteria was highly enriched in diabetic compared to non-diabetic persons (P = 0.02) and positively correlated with plasma glucose (P = 0.04).ConclusionsThe results of this study indicate that type 2 diabetes in humans is associated with compositional changes in intestinal microbiota. The level of glucose tolerance should be considered when linking microbiota with metabolic diseases such as obesity and developing strategies to control metabolic diseases by modifying the gut microbiota.

AB - BackgroundRecent evidence suggests that there is a link between metabolic diseases and bacterial populations in the gut. The aim of this study was to assess the differences between the composition of the intestinal microbiota in humans with type 2 diabetes and non-diabetic persons as control.Methods and FindingsThe study included 36 male adults with a broad range of age and body-mass indices (BMIs), among which 18 subjects were diagnosed with diabetes type 2. The fecal bacterial composition was investigated by real-time quantitative PCR (qPCR) and in a subgroup of subjects (N = 20) by tag-encoded amplicon pyrosequencing of the V4 region of the 16S rRNA gene. The proportions of phylum Firmicutes and class Clostridia were significantly reduced in the diabetic group compared to the control group (P = 0.03). Furthermore, the ratios of Bacteroidetes to Firmicutes as well as the ratios of Bacteroides-Prevotella group to C. coccoides-E. rectale group correlated positively and significantly with plasma glucose concentration (P = 0.04) but not with BMIs. Similarly, class Betaproteobacteria was highly enriched in diabetic compared to non-diabetic persons (P = 0.02) and positively correlated with plasma glucose (P = 0.04).ConclusionsThe results of this study indicate that type 2 diabetes in humans is associated with compositional changes in intestinal microbiota. The level of glucose tolerance should be considered when linking microbiota with metabolic diseases such as obesity and developing strategies to control metabolic diseases by modifying the gut microbiota.

U2 - 10.1371/journal.pone.0009085

DO - 10.1371/journal.pone.0009085

M3 - Journal article

VL - 5

SP - e9085

JO - P L o S One

JF - P L o S One

SN - 1932-6203

IS - 2

ER -

ID: 18389372