Gel-based phosphoproteomics analysis of sarcoplasmic proteins in postmortem porcine muscle with pH decline rate and time differences

Research output: Contribution to journalJournal articleResearchpeer-review

  • Honggang Huang
  • Martin Røssel Larsen
  • Anders H Karlsson
  • Luigi Pomponio
  • Leonardo Nanni Costa
  • Lametsch, Rene
Meat quality development is highly influenced by the pH decline caused by the postmortem (PM) glycolysis. Protein phosphorylation is an important mechanism in regulating the activity of glycometabolic enzymes. Here, a gel-based phosphoproteomic study was performed to analyze the protein phosphorylation in sarcoplasmic proteins from three groups of pigs with different pH decline rates from PM 1 to 24¿h. Globally, the fast pH decline group had the highest phosphorylation level at PM 1¿h, but lowest at 24¿h, whereas the slow pH decline group showed the reverse case. The same pattern was also observed in most individual bands in 1-DE. The protein phosphorylation levels of 12 bands were significantly affected by the synergy effects of pH and time (p
Original languageEnglish
JournalProteomics - Practical Proteomics
Issue number20
Pages (from-to)4063-4076
Number of pages14
Publication statusPublished - 2011

    Research areas

  • Animals, Diamond, Hydrogen-Ion Concentration, Muscle, Skeletal, Phosphorylation, Postmortem Changes, Proteomics, Quality Control, Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization, Staining and Labeling, Swine, Time Factors

ID: 37927670