Freeze/thaw effects on lipid-bilayer vesicles investigated by differential scanning calorimetry

Research output: Contribution to journalJournal articlepeer-review

Standard

Freeze/thaw effects on lipid-bilayer vesicles investigated by differential scanning calorimetry. / Kaasgaard, Thomas; Mouritsen, Ole G.; Jørgensen, Kent.

In: Biochimica et Biophysica Acta - Biomembranes, Vol. 1615, No. 1-2, 02.09.2003, p. 77-83.

Research output: Contribution to journalJournal articlepeer-review

Harvard

Kaasgaard, T, Mouritsen, OG & Jørgensen, K 2003, 'Freeze/thaw effects on lipid-bilayer vesicles investigated by differential scanning calorimetry', Biochimica et Biophysica Acta - Biomembranes, vol. 1615, no. 1-2, pp. 77-83. https://doi.org/10.1016/S0005-2736(03)00194-9

APA

Kaasgaard, T., Mouritsen, O. G., & Jørgensen, K. (2003). Freeze/thaw effects on lipid-bilayer vesicles investigated by differential scanning calorimetry. Biochimica et Biophysica Acta - Biomembranes, 1615(1-2), 77-83. https://doi.org/10.1016/S0005-2736(03)00194-9

Vancouver

Kaasgaard T, Mouritsen OG, Jørgensen K. Freeze/thaw effects on lipid-bilayer vesicles investigated by differential scanning calorimetry. Biochimica et Biophysica Acta - Biomembranes. 2003 Sep 2;1615(1-2):77-83. https://doi.org/10.1016/S0005-2736(03)00194-9

Author

Kaasgaard, Thomas ; Mouritsen, Ole G. ; Jørgensen, Kent. / Freeze/thaw effects on lipid-bilayer vesicles investigated by differential scanning calorimetry. In: Biochimica et Biophysica Acta - Biomembranes. 2003 ; Vol. 1615, No. 1-2. pp. 77-83.

Bibtex

@article{c168a4dfba9147659bdd2f2b3c7dd605,
title = "Freeze/thaw effects on lipid-bilayer vesicles investigated by differential scanning calorimetry",
abstract = "Differential scanning calorimetry (DSC) has been used to study the effects of repeated freezing and thawing on dipalmitoylphosphatidylcholine (DPPC) vesicles. Aqueous suspensions of both multilamellar vesicles (MLVs) and large unilamellar vesicles (LUVs) were cycled between -37 and 8°C, and for each thawing event, the enthalpy of ice-melting was measured. In the case of MLVs, the enthalpy increased each time the vesicles were thawed until a steady state was attained. In contrast, the enthalpies measured for LUV suspensions were independent of the number of previous thawing events. It was concluded that MLVs in terms of freezing characteristics contain two pools of water, namely bulk water and interlamellar water. Interlamellar water does not freeze under the conditions employed in the present study, and the MLVs therefore experience freeze-induced dehydration, which is the reason for the observed increase in ice-melting enthalpy. Furthermore, the thermodynamic results suggest that the osmotic stress resulting from the freeze-induced dehydration changes the lamellarity of the MLVs.",
keywords = "Freezing, Lipid bilayer, Multilamellar vesicle, Thawing, Unilamellar vesicle",
author = "Thomas Kaasgaard and Mouritsen, {Ole G.} and Kent J{\o}rgensen",
year = "2003",
month = sep,
day = "2",
doi = "10.1016/S0005-2736(03)00194-9",
language = "English",
volume = "1615",
pages = "77--83",
journal = "B B A - Biomembranes",
issn = "0005-2736",
publisher = "Elsevier",
number = "1-2",

}

RIS

TY - JOUR

T1 - Freeze/thaw effects on lipid-bilayer vesicles investigated by differential scanning calorimetry

AU - Kaasgaard, Thomas

AU - Mouritsen, Ole G.

AU - Jørgensen, Kent

PY - 2003/9/2

Y1 - 2003/9/2

N2 - Differential scanning calorimetry (DSC) has been used to study the effects of repeated freezing and thawing on dipalmitoylphosphatidylcholine (DPPC) vesicles. Aqueous suspensions of both multilamellar vesicles (MLVs) and large unilamellar vesicles (LUVs) were cycled between -37 and 8°C, and for each thawing event, the enthalpy of ice-melting was measured. In the case of MLVs, the enthalpy increased each time the vesicles were thawed until a steady state was attained. In contrast, the enthalpies measured for LUV suspensions were independent of the number of previous thawing events. It was concluded that MLVs in terms of freezing characteristics contain two pools of water, namely bulk water and interlamellar water. Interlamellar water does not freeze under the conditions employed in the present study, and the MLVs therefore experience freeze-induced dehydration, which is the reason for the observed increase in ice-melting enthalpy. Furthermore, the thermodynamic results suggest that the osmotic stress resulting from the freeze-induced dehydration changes the lamellarity of the MLVs.

AB - Differential scanning calorimetry (DSC) has been used to study the effects of repeated freezing and thawing on dipalmitoylphosphatidylcholine (DPPC) vesicles. Aqueous suspensions of both multilamellar vesicles (MLVs) and large unilamellar vesicles (LUVs) were cycled between -37 and 8°C, and for each thawing event, the enthalpy of ice-melting was measured. In the case of MLVs, the enthalpy increased each time the vesicles were thawed until a steady state was attained. In contrast, the enthalpies measured for LUV suspensions were independent of the number of previous thawing events. It was concluded that MLVs in terms of freezing characteristics contain two pools of water, namely bulk water and interlamellar water. Interlamellar water does not freeze under the conditions employed in the present study, and the MLVs therefore experience freeze-induced dehydration, which is the reason for the observed increase in ice-melting enthalpy. Furthermore, the thermodynamic results suggest that the osmotic stress resulting from the freeze-induced dehydration changes the lamellarity of the MLVs.

KW - Freezing

KW - Lipid bilayer

KW - Multilamellar vesicle

KW - Thawing

KW - Unilamellar vesicle

UR - http://www.scopus.com/inward/record.url?scp=0042510132&partnerID=8YFLogxK

U2 - 10.1016/S0005-2736(03)00194-9

DO - 10.1016/S0005-2736(03)00194-9

M3 - Journal article

C2 - 12948589

AN - SCOPUS:0042510132

VL - 1615

SP - 77

EP - 83

JO - B B A - Biomembranes

JF - B B A - Biomembranes

SN - 0005-2736

IS - 1-2

ER -

ID: 230986244