Engineering of plastids to optimize the production of high-value metabolites and proteins

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Engineering of plastids to optimize the production of high-value metabolites and proteins. / Jensen, Poul Erik; Scharff, Lars B.

In: Current Opinion in Biotechnology, Vol. 59, 10.2019, p. 8-15.

Research output: Contribution to journal › Review › Research › peer-review

Harvard

Jensen, PE & Scharff, LB 2019, 'Engineering of plastids to optimize the production of high-value metabolites and proteins', Current Opinion in Biotechnology, vol. 59, pp. 8-15. https://doi.org/10.1016/j.copbio.2019.01.009

APA

Jensen, P. E., & Scharff, L. B. (2019). Engineering of plastids to optimize the production of high-value metabolites and proteins. Current Opinion in Biotechnology, 59, 8-15. https://doi.org/10.1016/j.copbio.2019.01.009

Vancouver

Jensen PE, Scharff LB. Engineering of plastids to optimize the production of high-value metabolites and proteins. Current Opinion in Biotechnology. 2019 Oct;59:8-15. https://doi.org/10.1016/j.copbio.2019.01.009

Author

Jensen, Poul Erik ; Scharff, Lars B. / Engineering of plastids to optimize the production of high-value metabolites and proteins. In: Current Opinion in Biotechnology. 2019 ; Vol. 59. pp. 8-15.

Bibtex

@article{d950b94c68b04dc4bd67f7ed8c1d4417,

title = "Engineering of plastids to optimize the production of high-value metabolites and proteins",

abstract = "Plastids are interesting targets for metabolic engineering using the tools of synthetic biology. Plastids carry their own genome, which can be manipulated genetically in many algae and plants. Incorporating foreign genes into the plastid genome offers valuable benefits, such as high-level foreign protein expression and the absence of gene silencing. Here, we review progress in bioengineering of chloroplasts to produce valuable metabolites and proteins. Various strategies for enhancing yields of desired products, including design of operons, fusion proteins for improved translational efficiency, protein scaffolding, metabolic channeling and storage, are described. Efforts to control plastid differentiation also offer promising ways of turning plastids into controllable bio-factories, and the construction of synthetic plastids optimized for specific functions would be a major advance.",

author = "Jensen, {Poul Erik} and Scharff, {Lars B}",

year = "2019",

month = oct,

doi = "10.1016/j.copbio.2019.01.009",

language = "English",

volume = "59",

pages = "8--15",

journal = "Current Opinion in Biotechnology",

issn = "0958-1669",

publisher = "Elsevier Ltd. * Current Opinion Journals",

}

RIS

TY - JOUR

T1 - Engineering of plastids to optimize the production of high-value metabolites and proteins

AU - Jensen, Poul Erik

AU - Scharff, Lars B

PY - 2019/10

Y1 - 2019/10

N2 - Plastids are interesting targets for metabolic engineering using the tools of synthetic biology. Plastids carry their own genome, which can be manipulated genetically in many algae and plants. Incorporating foreign genes into the plastid genome offers valuable benefits, such as high-level foreign protein expression and the absence of gene silencing. Here, we review progress in bioengineering of chloroplasts to produce valuable metabolites and proteins. Various strategies for enhancing yields of desired products, including design of operons, fusion proteins for improved translational efficiency, protein scaffolding, metabolic channeling and storage, are described. Efforts to control plastid differentiation also offer promising ways of turning plastids into controllable bio-factories, and the construction of synthetic plastids optimized for specific functions would be a major advance.

AB - Plastids are interesting targets for metabolic engineering using the tools of synthetic biology. Plastids carry their own genome, which can be manipulated genetically in many algae and plants. Incorporating foreign genes into the plastid genome offers valuable benefits, such as high-level foreign protein expression and the absence of gene silencing. Here, we review progress in bioengineering of chloroplasts to produce valuable metabolites and proteins. Various strategies for enhancing yields of desired products, including design of operons, fusion proteins for improved translational efficiency, protein scaffolding, metabolic channeling and storage, are described. Efforts to control plastid differentiation also offer promising ways of turning plastids into controllable bio-factories, and the construction of synthetic plastids optimized for specific functions would be a major advance.

U2 - 10.1016/j.copbio.2019.01.009

DO - 10.1016/j.copbio.2019.01.009

M3 - Review

C2 - 30798145

VL - 59

SP - 8

EP - 15

JO - Current Opinion in Biotechnology

JF - Current Opinion in Biotechnology

SN - 0958-1669

ER -

ID: 213957654

Department of Food Science