Effects of Lactococcus lactis on composition of intestinal microbiota: role of nisin.

Research output: Contribution to journalJournal articleResearchpeer-review

Nete Bernbom, Tine Rask Licht, Carl-Henrik Brogren, Birthe Jelle, Anette H Johansen, Iker Badiola, Finn K Vogensen, Birgit Nørrung

This study examined the ability of (i) pure nisin, (ii) nisin-producing Lactococcus lactis strain CHCC5826, and (iii) the non-nisin-producing L. lactis strain CHCH2862 to affect the composition of the intestinal microbiota of human flora-associated rats. The presence of both the nisin-producing and the non-nisin-producing L. lactis strains significantly increased the number of Bifidobacterium cells in fecal samples during the first 8 days but decreased the number of enterococci/streptococci in duodenum, ileum, cecum, and colon samples as detected by selective cultivation. No significant changes in the rat fecal microbiota were observed after dosage with nisin. Pearson cluster analysis of denaturing gradient gel electrophoresis profiles of the 16S rRNA genes present in the fecal microbial population revealed that the microbiota of animals dosed with either of the two L. lactis strains were different from that of control animals dosed with saline. However, profiles of the microbiota from animals dosed with nisin did not differ from the controls. The concentrations of nisin estimated by competitive enzyme-linked immunosorbent assay (ELISA) were approximately 10-fold higher in the small intestine and 200-fold higher in feces than the corresponding concentrations estimated by a biological assay. This indicates that nisin was degraded or inactivated in the gastrointestinal tract, since fragments of this bacteriocin are detected by ELISA while an intact molecule is needed to retain biological activity.
Original languageEnglish
JournalApplied and Environmental Microbiology
Volume72
Issue number1
Pages (from-to)239-44
Number of pages5
ISSN0099-2240
DOIs
Publication statusPublished - 2006

Bibliographical note

Keywords: Agar; Animals; Bifidobacterium; Culture Media; Enterococcus; Feces; Germ-Free Life; Humans; Intestines; Lactococcus lactis; Nisin; Rats; Rats, Sprague-Dawley; Streptococcus

ID: 8463154