Comparison of methods and animal models commonly used for investigation of fecal microbiota: effects of time, host and gender

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

Comparison of methods and animal models commonly used for investigation of fecal microbiota : effects of time, host and gender. / Bernbom, Nete; Nørrung, Birgit; Saadbye, Peter; Mølbak, Lars; Vogensen, Finn Kvist; Licht, Tine Rask.

In: Journal of Microbiological Methods, Vol. 66, No. 1, 2006, p. 87-95.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Bernbom, N, Nørrung, B, Saadbye, P, Mølbak, L, Vogensen, FK & Licht, TR 2006, 'Comparison of methods and animal models commonly used for investigation of fecal microbiota: effects of time, host and gender', Journal of Microbiological Methods, vol. 66, no. 1, pp. 87-95. https://doi.org/10.1016/j.mimet.2005.10.014

APA

Bernbom, N., Nørrung, B., Saadbye, P., Mølbak, L., Vogensen, F. K., & Licht, T. R. (2006). Comparison of methods and animal models commonly used for investigation of fecal microbiota: effects of time, host and gender. Journal of Microbiological Methods, 66(1), 87-95. https://doi.org/10.1016/j.mimet.2005.10.014

Vancouver

Bernbom N, Nørrung B, Saadbye P, Mølbak L, Vogensen FK, Licht TR. Comparison of methods and animal models commonly used for investigation of fecal microbiota: effects of time, host and gender. Journal of Microbiological Methods. 2006;66(1):87-95. https://doi.org/10.1016/j.mimet.2005.10.014

Author

Bernbom, Nete ; Nørrung, Birgit ; Saadbye, Peter ; Mølbak, Lars ; Vogensen, Finn Kvist ; Licht, Tine Rask. / Comparison of methods and animal models commonly used for investigation of fecal microbiota : effects of time, host and gender. In: Journal of Microbiological Methods. 2006 ; Vol. 66, No. 1. pp. 87-95.

Bibtex

@article{0cbd2fd0a1c411ddb6ae000ea68e967b,
title = "Comparison of methods and animal models commonly used for investigation of fecal microbiota: effects of time, host and gender",
abstract = "Denaturing gradient gel electrophoresis (DGGE), terminal restriction fragment length polymorphism (T-RFLP) and plating on selective agars were used to study variation in the fecal microbiota of rats over time as well as variation between individuals. Investigated rats were either conventional and specific pathogen free (SPF), or human flora associated (HFA). A higher variation (p < 0.05) in fecal microbiota over time was observed for HFA than for SPF animals. Analysis of DGGE and T-RFLP profiles of fecal microbiota from SPF and HFA rats revealed that variation over time was less significant than variation between individuals, and that phylogenetic profiles clustered according to gender. These observations should be taken into account when designing future research addressing changes in fecal microbiota.",
keywords = "Former LIFE faculty, DGGE; T-RFLP; Fecal microbiota; Community analysis",
author = "Nete Bernbom and Birgit N{\o}rrung and Peter Saadbye and Lars M{\o}lbak and Vogensen, {Finn Kvist} and Licht, {Tine Rask}",
year = "2006",
doi = "10.1016/j.mimet.2005.10.014",
language = "English",
volume = "66",
pages = "87--95",
journal = "Journal of Microbiological Methods",
issn = "0167-7012",
publisher = "Elsevier",
number = "1",

}

RIS

TY - JOUR

T1 - Comparison of methods and animal models commonly used for investigation of fecal microbiota

T2 - effects of time, host and gender

AU - Bernbom, Nete

AU - Nørrung, Birgit

AU - Saadbye, Peter

AU - Mølbak, Lars

AU - Vogensen, Finn Kvist

AU - Licht, Tine Rask

PY - 2006

Y1 - 2006

N2 - Denaturing gradient gel electrophoresis (DGGE), terminal restriction fragment length polymorphism (T-RFLP) and plating on selective agars were used to study variation in the fecal microbiota of rats over time as well as variation between individuals. Investigated rats were either conventional and specific pathogen free (SPF), or human flora associated (HFA). A higher variation (p < 0.05) in fecal microbiota over time was observed for HFA than for SPF animals. Analysis of DGGE and T-RFLP profiles of fecal microbiota from SPF and HFA rats revealed that variation over time was less significant than variation between individuals, and that phylogenetic profiles clustered according to gender. These observations should be taken into account when designing future research addressing changes in fecal microbiota.

AB - Denaturing gradient gel electrophoresis (DGGE), terminal restriction fragment length polymorphism (T-RFLP) and plating on selective agars were used to study variation in the fecal microbiota of rats over time as well as variation between individuals. Investigated rats were either conventional and specific pathogen free (SPF), or human flora associated (HFA). A higher variation (p < 0.05) in fecal microbiota over time was observed for HFA than for SPF animals. Analysis of DGGE and T-RFLP profiles of fecal microbiota from SPF and HFA rats revealed that variation over time was less significant than variation between individuals, and that phylogenetic profiles clustered according to gender. These observations should be taken into account when designing future research addressing changes in fecal microbiota.

KW - Former LIFE faculty

KW - DGGE; T-RFLP; Fecal microbiota; Community analysis

U2 - 10.1016/j.mimet.2005.10.014

DO - 10.1016/j.mimet.2005.10.014

M3 - Journal article

VL - 66

SP - 87

EP - 95

JO - Journal of Microbiological Methods

JF - Journal of Microbiological Methods

SN - 0167-7012

IS - 1

ER -

ID: 8108394