Characterization of the Vaginal DNA Virome in Health and Dysbiosis
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Characterization of the Vaginal DNA Virome in Health and Dysbiosis. / Jakobsen, Rasmus Riemer; Haahr, Thor; Humaidan, Peter; Jensen, Jørgen Skov; Kot, Witold Piotr; Castro-Mejia, Josue Leonardo; Deng, Ling; Leser, Thomas Dyrmann; Nielsen, Dennis Sandris.
In: Viruses, Vol. 12, No. 10, 1143, 2020.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Characterization of the Vaginal DNA Virome in Health and Dysbiosis
AU - Jakobsen, Rasmus Riemer
AU - Haahr, Thor
AU - Humaidan, Peter
AU - Jensen, Jørgen Skov
AU - Kot, Witold Piotr
AU - Castro-Mejia, Josue Leonardo
AU - Deng, Ling
AU - Leser, Thomas Dyrmann
AU - Nielsen, Dennis Sandris
PY - 2020
Y1 - 2020
N2 - Bacterial vaginosis (BV) is characterized by a reduction in Lactobacillus (L.) spp. abundance and increased abundance of facultative anaerobes, such as Gardnerella spp. BV aetiology is not fully understood; however, bacteriophages could play a pivotal role in the perturbation of the vaginal bacterial community. We investigated the vaginal viral community, including bacteriophages and the association to the bacterial community and BV-status. Vaginal samples from 48 patients undergoing IVF treatment for non-female factor infertility were subjected to metagenomic sequencing of purified virus-like particles. The vaginal viral community was characterized and correlated with the BV-status by Nugent score, bacterial community, structure, and the presence of key vaginal bacterial species. The majority of identified vaginal viruses belonged to the class of double-stranded DNA bacteriophages, with eukaryotic viruses constituting 4% of the total reads. Clear links between the viral community composition and BV (q = 0.006, R = 0.26) as well as the presence of L. crispatus (q = 0.001, R = 0.43), L. iners, Gardnerella spp., and Atopobium vaginae were found (q < 0.002, R > 0.15). The eukaryotic viral community also correlated with BV-status (q = 0.018, R = 0.20). In conclusion, the vaginal virome was clearly linked with bacterial community structure and BV-status.
AB - Bacterial vaginosis (BV) is characterized by a reduction in Lactobacillus (L.) spp. abundance and increased abundance of facultative anaerobes, such as Gardnerella spp. BV aetiology is not fully understood; however, bacteriophages could play a pivotal role in the perturbation of the vaginal bacterial community. We investigated the vaginal viral community, including bacteriophages and the association to the bacterial community and BV-status. Vaginal samples from 48 patients undergoing IVF treatment for non-female factor infertility were subjected to metagenomic sequencing of purified virus-like particles. The vaginal viral community was characterized and correlated with the BV-status by Nugent score, bacterial community, structure, and the presence of key vaginal bacterial species. The majority of identified vaginal viruses belonged to the class of double-stranded DNA bacteriophages, with eukaryotic viruses constituting 4% of the total reads. Clear links between the viral community composition and BV (q = 0.006, R = 0.26) as well as the presence of L. crispatus (q = 0.001, R = 0.43), L. iners, Gardnerella spp., and Atopobium vaginae were found (q < 0.002, R > 0.15). The eukaryotic viral community also correlated with BV-status (q = 0.018, R = 0.20). In conclusion, the vaginal virome was clearly linked with bacterial community structure and BV-status.
KW - Bacterial vaginosis
KW - Bacteriophages
KW - Dysbiosis
KW - Vaginal microbiome
KW - Vaginal virome
U2 - 10.3390/v12101143
DO - 10.3390/v12101143
M3 - Journal article
C2 - 33050261
AN - SCOPUS:85092476480
VL - 12
JO - Viruses
JF - Viruses
SN - 1999-4915
IS - 10
M1 - 1143
ER -
ID: 250915487