Characterization of Bacillus spp. strains for use as probiotic additives in pig feed

Research output: Contribution to journalJournal articlepeer-review

Standard

Characterization of Bacillus spp. strains for use as probiotic additives in pig feed. / Larsen, Nadja; Thorsen, Line; Kpikpi, Elmer Nayra; Stuer-Lauridsen, Birgitte; Cantor, Mette Dines; Nielsen, Bea; Brockmann, Elke; Derkx, Patrick M.F.; Jespersen, Lene.

In: Applied Microbiology and Biotechnology, Vol. 98, No. 3, 2014, p. 1105-1118.

Research output: Contribution to journalJournal articlepeer-review

Harvard

Larsen, N, Thorsen, L, Kpikpi, EN, Stuer-Lauridsen, B, Cantor, MD, Nielsen, B, Brockmann, E, Derkx, PMF & Jespersen, L 2014, 'Characterization of Bacillus spp. strains for use as probiotic additives in pig feed', Applied Microbiology and Biotechnology, vol. 98, no. 3, pp. 1105-1118. https://doi.org/10.1007/s00253-013-5343-6

APA

Larsen, N., Thorsen, L., Kpikpi, E. N., Stuer-Lauridsen, B., Cantor, M. D., Nielsen, B., Brockmann, E., Derkx, P. M. F., & Jespersen, L. (2014). Characterization of Bacillus spp. strains for use as probiotic additives in pig feed. Applied Microbiology and Biotechnology, 98(3), 1105-1118. https://doi.org/10.1007/s00253-013-5343-6

Vancouver

Larsen N, Thorsen L, Kpikpi EN, Stuer-Lauridsen B, Cantor MD, Nielsen B et al. Characterization of Bacillus spp. strains for use as probiotic additives in pig feed. Applied Microbiology and Biotechnology. 2014;98(3):1105-1118. https://doi.org/10.1007/s00253-013-5343-6

Author

Larsen, Nadja ; Thorsen, Line ; Kpikpi, Elmer Nayra ; Stuer-Lauridsen, Birgitte ; Cantor, Mette Dines ; Nielsen, Bea ; Brockmann, Elke ; Derkx, Patrick M.F. ; Jespersen, Lene. / Characterization of Bacillus spp. strains for use as probiotic additives in pig feed. In: Applied Microbiology and Biotechnology. 2014 ; Vol. 98, No. 3. pp. 1105-1118.

Bibtex

@article{f321ac960ad7462e9b25e1004c073f08,
title = "Characterization of Bacillus spp. strains for use as probiotic additives in pig feed",
abstract = "Bacillus spp. are commonly used as probiotic species in the feed industry, however, their benefits need to be confirmed. This study describes a high throughput screening combined with the detailed characterization of endospore-forming bacteria with the aim to identify new Bacillus spp. strains for use as probiotic additives in pig feed. A total of 245 bacterial isolates derived from African fermented food, feces and soil were identified by 16S rRNA gene sequencing and screened for antimicrobial activity and growth in the presence of antibiotics, bile salts and at pH 4.0. Thirty-three Bacillus spp. isolates with the best characteristics were identified by gyrB and rpoB gene sequencing as B. amyloliquefaciens subsp. plantarum, B. amyloliquefaciens subsp. amyloliquefaciens, B. subtilis subsp. subtilis, B. licheniformis, B. mojavensis, B. pumilus and B. megaterium. These isolates were further investigated for their activity against the pathogenic bacteria, antibiotic susceptibility, sporulation rates, biofilm formation and production of glycosyl hydrolytic enzymes. Additionally, ten selected isolates were assessed for heat resistance of spores and the effect on porcine epithelial cells IPEC-J2. Isolates of B. amyloliquefaciens, B. subtilis and B. mojavensis, showed the best overall characteristics and, therefore, potential for usage as probiotic additives in feed. A large number of taxonomically diverse strains made it possible to reveal species and subspecies-specific trends, contributing to our understanding of the probiotic potential of Bacillus species.",
author = "Nadja Larsen and Line Thorsen and Kpikpi, {Elmer Nayra} and Birgitte Stuer-Lauridsen and Cantor, {Mette Dines} and Bea Nielsen and Elke Brockmann and Derkx, {Patrick M.F.} and Lene Jespersen",
year = "2014",
doi = "10.1007/s00253-013-5343-6",
language = "English",
volume = "98",
pages = "1105--1118",
journal = "Applied Microbiology and Biotechnology",
issn = "0175-7598",
publisher = "Springer",
number = "3",

}

RIS

TY - JOUR

T1 - Characterization of Bacillus spp. strains for use as probiotic additives in pig feed

AU - Larsen, Nadja

AU - Thorsen, Line

AU - Kpikpi, Elmer Nayra

AU - Stuer-Lauridsen, Birgitte

AU - Cantor, Mette Dines

AU - Nielsen, Bea

AU - Brockmann, Elke

AU - Derkx, Patrick M.F.

AU - Jespersen, Lene

PY - 2014

Y1 - 2014

N2 - Bacillus spp. are commonly used as probiotic species in the feed industry, however, their benefits need to be confirmed. This study describes a high throughput screening combined with the detailed characterization of endospore-forming bacteria with the aim to identify new Bacillus spp. strains for use as probiotic additives in pig feed. A total of 245 bacterial isolates derived from African fermented food, feces and soil were identified by 16S rRNA gene sequencing and screened for antimicrobial activity and growth in the presence of antibiotics, bile salts and at pH 4.0. Thirty-three Bacillus spp. isolates with the best characteristics were identified by gyrB and rpoB gene sequencing as B. amyloliquefaciens subsp. plantarum, B. amyloliquefaciens subsp. amyloliquefaciens, B. subtilis subsp. subtilis, B. licheniformis, B. mojavensis, B. pumilus and B. megaterium. These isolates were further investigated for their activity against the pathogenic bacteria, antibiotic susceptibility, sporulation rates, biofilm formation and production of glycosyl hydrolytic enzymes. Additionally, ten selected isolates were assessed for heat resistance of spores and the effect on porcine epithelial cells IPEC-J2. Isolates of B. amyloliquefaciens, B. subtilis and B. mojavensis, showed the best overall characteristics and, therefore, potential for usage as probiotic additives in feed. A large number of taxonomically diverse strains made it possible to reveal species and subspecies-specific trends, contributing to our understanding of the probiotic potential of Bacillus species.

AB - Bacillus spp. are commonly used as probiotic species in the feed industry, however, their benefits need to be confirmed. This study describes a high throughput screening combined with the detailed characterization of endospore-forming bacteria with the aim to identify new Bacillus spp. strains for use as probiotic additives in pig feed. A total of 245 bacterial isolates derived from African fermented food, feces and soil were identified by 16S rRNA gene sequencing and screened for antimicrobial activity and growth in the presence of antibiotics, bile salts and at pH 4.0. Thirty-three Bacillus spp. isolates with the best characteristics were identified by gyrB and rpoB gene sequencing as B. amyloliquefaciens subsp. plantarum, B. amyloliquefaciens subsp. amyloliquefaciens, B. subtilis subsp. subtilis, B. licheniformis, B. mojavensis, B. pumilus and B. megaterium. These isolates were further investigated for their activity against the pathogenic bacteria, antibiotic susceptibility, sporulation rates, biofilm formation and production of glycosyl hydrolytic enzymes. Additionally, ten selected isolates were assessed for heat resistance of spores and the effect on porcine epithelial cells IPEC-J2. Isolates of B. amyloliquefaciens, B. subtilis and B. mojavensis, showed the best overall characteristics and, therefore, potential for usage as probiotic additives in feed. A large number of taxonomically diverse strains made it possible to reveal species and subspecies-specific trends, contributing to our understanding of the probiotic potential of Bacillus species.

U2 - 10.1007/s00253-013-5343-6

DO - 10.1007/s00253-013-5343-6

M3 - Journal article

C2 - 24201893

VL - 98

SP - 1105

EP - 1118

JO - Applied Microbiology and Biotechnology

JF - Applied Microbiology and Biotechnology

SN - 0175-7598

IS - 3

ER -

ID: 97519086