Bacterial flow cytometry and imaging as potential process monitoring tools for industrial biotechnology

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

Bacterial flow cytometry and imaging as potential process monitoring tools for industrial biotechnology. / Kadamalakunte Narayana, Sumana; Mallick, Sanjaya; Siegumfeldt, Henrik; van den Berg, Frans.

In: Fermentation, Vol. 6, No. 1, 10, 2020.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Kadamalakunte Narayana, S, Mallick, S, Siegumfeldt, H & van den Berg, F 2020, 'Bacterial flow cytometry and imaging as potential process monitoring tools for industrial biotechnology', Fermentation, vol. 6, no. 1, 10. https://doi.org/10.3390/fermentation6010010

APA

Kadamalakunte Narayana, S., Mallick, S., Siegumfeldt, H., & van den Berg, F. (2020). Bacterial flow cytometry and imaging as potential process monitoring tools for industrial biotechnology. Fermentation, 6(1), [10]. https://doi.org/10.3390/fermentation6010010

Vancouver

Kadamalakunte Narayana S, Mallick S, Siegumfeldt H, van den Berg F. Bacterial flow cytometry and imaging as potential process monitoring tools for industrial biotechnology. Fermentation. 2020;6(1). 10. https://doi.org/10.3390/fermentation6010010

Author

Kadamalakunte Narayana, Sumana ; Mallick, Sanjaya ; Siegumfeldt, Henrik ; van den Berg, Frans. / Bacterial flow cytometry and imaging as potential process monitoring tools for industrial biotechnology. In: Fermentation. 2020 ; Vol. 6, No. 1.

Bibtex

@article{4d8e3e6b137f40dcbacd785600c7cc37,
title = "Bacterial flow cytometry and imaging as potential process monitoring tools for industrial biotechnology",
abstract = "Minimizing process variations by early identification of deviations is one approach to make industrial production processes robust. Cell morphology is a direct representation of the physiological state and an important factor for the cell{\textquoteright}s survival in harsh environments as encountered during industrial processing. The adverse effects of fluctuating process parameters on cells were studied using flow cytometry and imaging. Results showed that altered pH caused a shift in cell size distribution from a heterogeneous mix of elongated and short cells to a homogenous population of short cells. Staining based on membrane integrity revealed a dynamics in the pattern of cluster formation during fermentation. Contradictory findings from forward scatter and imaging highlight the need for use of complementary techniques that provide visual confirmation to interpret changes. An atline flow cytometry or imaging capable of identifying subtle population deviations serves as a powerful monitoring tool for industrial biotechnology.",
keywords = "Cell size, Flow cytometry, Forward scatter, Imaging, Industrial biotechnology, Lactobacillus acidophilus, Microscopy, Viability",
author = "{Kadamalakunte Narayana}, Sumana and Sanjaya Mallick and Henrik Siegumfeldt and {van den Berg}, Frans",
year = "2020",
doi = "10.3390/fermentation6010010",
language = "English",
volume = "6",
journal = "Fermentation",
issn = "2311-5637",
publisher = "Multidisciplinary Digital Publishing Institute",
number = "1",

}

RIS

TY - JOUR

T1 - Bacterial flow cytometry and imaging as potential process monitoring tools for industrial biotechnology

AU - Kadamalakunte Narayana, Sumana

AU - Mallick, Sanjaya

AU - Siegumfeldt, Henrik

AU - van den Berg, Frans

PY - 2020

Y1 - 2020

N2 - Minimizing process variations by early identification of deviations is one approach to make industrial production processes robust. Cell morphology is a direct representation of the physiological state and an important factor for the cell’s survival in harsh environments as encountered during industrial processing. The adverse effects of fluctuating process parameters on cells were studied using flow cytometry and imaging. Results showed that altered pH caused a shift in cell size distribution from a heterogeneous mix of elongated and short cells to a homogenous population of short cells. Staining based on membrane integrity revealed a dynamics in the pattern of cluster formation during fermentation. Contradictory findings from forward scatter and imaging highlight the need for use of complementary techniques that provide visual confirmation to interpret changes. An atline flow cytometry or imaging capable of identifying subtle population deviations serves as a powerful monitoring tool for industrial biotechnology.

AB - Minimizing process variations by early identification of deviations is one approach to make industrial production processes robust. Cell morphology is a direct representation of the physiological state and an important factor for the cell’s survival in harsh environments as encountered during industrial processing. The adverse effects of fluctuating process parameters on cells were studied using flow cytometry and imaging. Results showed that altered pH caused a shift in cell size distribution from a heterogeneous mix of elongated and short cells to a homogenous population of short cells. Staining based on membrane integrity revealed a dynamics in the pattern of cluster formation during fermentation. Contradictory findings from forward scatter and imaging highlight the need for use of complementary techniques that provide visual confirmation to interpret changes. An atline flow cytometry or imaging capable of identifying subtle population deviations serves as a powerful monitoring tool for industrial biotechnology.

KW - Cell size

KW - Flow cytometry

KW - Forward scatter

KW - Imaging

KW - Industrial biotechnology

KW - Lactobacillus acidophilus

KW - Microscopy

KW - Viability

U2 - 10.3390/fermentation6010010

DO - 10.3390/fermentation6010010

M3 - Journal article

AN - SCOPUS:85080044875

VL - 6

JO - Fermentation

JF - Fermentation

SN - 2311-5637

IS - 1

M1 - 10

ER -

ID: 238677463