A Bacteriophage Cocktail Significantly Reduces Listeria monocytogenes without Deleterious Impact on the Commensal Gut Microbiota under Simulated Gastrointestinal Conditions
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A Bacteriophage Cocktail Significantly Reduces Listeria monocytogenes without Deleterious Impact on the Commensal Gut Microbiota under Simulated Gastrointestinal Conditions. / Jakobsen, Rasmus Riemer; Trinh, Jimmy T.; Bomholtz, Louise; Brok-Lauridsen, Signe Kristine; Sulakvelidze, Alexander; Nielsen, Dennis Sandris.
In: Viruses, Vol. 14, No. 2, 190, 2022.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - A Bacteriophage Cocktail Significantly Reduces Listeria monocytogenes without Deleterious Impact on the Commensal Gut Microbiota under Simulated Gastrointestinal Conditions
AU - Jakobsen, Rasmus Riemer
AU - Trinh, Jimmy T.
AU - Bomholtz, Louise
AU - Brok-Lauridsen, Signe Kristine
AU - Sulakvelidze, Alexander
AU - Nielsen, Dennis Sandris
N1 - Funding Information: Funding: Funding for the study was provided by Intralytix, Inc. Publisher Copyright: © 2022 by the authors. Licensee MDPI, Basel, Switzerland.
PY - 2022
Y1 - 2022
N2 - In this study, we examined the effect of a bacteriophage cocktail (tentatively designated as the Foodborne Outbreak Pill (FOP)) on the levels of Listeria monocytogenes in simulated small intestine, large intestine, and Caco-2 model systems. We found that FOP survival during simulated passage of the upper gastrointestinal was dependent on stomach pH, and that FOP robustly inhibited L. monocytogenes levels with effectiveness comparable to antibiotic treatment (ampicillin) under simulated ilium and colon conditions. The FOP did not inhibit the commensal bacteria, whereas ampicillin treatment led to dysbiosis-like conditions. The FOP was also more effective than an antibiotic in protecting Caco-2 cells from adhesion and invasion by L. monocytogenes (5-log reduction vs. 1-log reduction) while not triggering an inflammatory response. Our data suggested that the FOP may provide a robust protection against L. monocytogenes should the bacterium enter the human gastrointestinal tract (e.g., by consumption of contaminated food), without deleterious impact on the commensal bacteria.
AB - In this study, we examined the effect of a bacteriophage cocktail (tentatively designated as the Foodborne Outbreak Pill (FOP)) on the levels of Listeria monocytogenes in simulated small intestine, large intestine, and Caco-2 model systems. We found that FOP survival during simulated passage of the upper gastrointestinal was dependent on stomach pH, and that FOP robustly inhibited L. monocytogenes levels with effectiveness comparable to antibiotic treatment (ampicillin) under simulated ilium and colon conditions. The FOP did not inhibit the commensal bacteria, whereas ampicillin treatment led to dysbiosis-like conditions. The FOP was also more effective than an antibiotic in protecting Caco-2 cells from adhesion and invasion by L. monocytogenes (5-log reduction vs. 1-log reduction) while not triggering an inflammatory response. Our data suggested that the FOP may provide a robust protection against L. monocytogenes should the bacterium enter the human gastrointestinal tract (e.g., by consumption of contaminated food), without deleterious impact on the commensal bacteria.
KW - Gut microbiome
KW - Listeria monocytogenes
KW - Phage therapy
U2 - 10.3390/v14020190
DO - 10.3390/v14020190
M3 - Journal article
C2 - 35215782
AN - SCOPUS:85123062674
VL - 14
JO - Viruses
JF - Viruses
SN - 1999-4915
IS - 2
M1 - 190
ER -
ID: 291806458