UHPLC Lab
Description
The (U)HPLC platform in the Ingredient and Dairy Technology section consists of several UHPLC and HPLC systems equipped with UV-Vis, fluorescence, and refractive index detectors. These instruments can be used to identify and quantify a wide range of analytes via reversed-phase, ion exchange, and size exclusion chromatography. The platform also includes two dedicated UHPLCs with UV-Vis and fluorescence detectors connected in series, enabling multidimensional identification and quantification of analytes. The platform supports a variety of analyses, including but not limited to:
- protein and free amino acid composition through automated in-needle derivatization
- umami free amino acids and nucleotides
- intact proteins
- polyphenols
- protein oxidation products
- sugars and organic acids
- vitamin C
- alpha-dicarbonyl compounds
- carotenoids
Options and requirements for use
- Using this platform requires extensive training from qualified personnel, and users are only allowed to handle the instruments after completing this training provided by UCPH FOOD staff.
- Assistance with sample preparation, experimental design, and data processing is available upon request.
- Instrument booking must be made in advance in agreement with the lab responsible.
At the start of a collaboration on research with the University of Copenhagen, a cooperation agreement must be drawn up. There are different types of agreements depending on the nature of the cooperation. See more here: collaboration.ku.dk/cooperation-agreements/
Associate Professor Mahesha M. Poojary (mahesha@food.ku.dk)
Lab technician Bente Pia Danielsen (bsoe@food.ku.dk)
Lab technician Henriette Rifbjerg Erichsen (hre@food.ku.dk)