NMR Platform

Description

The food NMR platform consists of three NMR spectrometers, which can be used for the untargeted screening of various sample types, ranging from foods to biofluids. It allows for:

  • Investigating different nuclei in the molecules (i.e., 1H, 13C, 31P etc.)
  • Monitoring reaction kinetics (i.e., food fermentations)
  • Elucidating chemical structures of unknown molecules – level 1 identification

The food NMR platform consists of three NMR spectrometers, Avance III 600 and 500 MHz from Bruker Biospin, and Benchtop NMR Spinsolve 80 MHz from Magritek as well as an automatic liquid handler (SamplePro Tube, Bruker Biospin).

Bruker Avance III™ 600

  • Suitable for the analysis of liquid samples
  • Suitable for studies with large sample sizes (>100 samples)
  • Equipped with an automated sample changer (SampleJet, Bruker Biospin), which can host up to 480 samples and has a refrigerated sample storage station.
  • Extraction or solubilization required prior to analyzing semi-solid or solid samples

Bruker Avance III™ 500

  • Suitable for the analysis of liquid samples
  • Equipped with an automated sample changer (Sample Xpress, Bruker Biospin), which can host up to 60 samples – no refrigerated sample storage station.
  • Extraction or solubilization required prior to analyzing semi-solid or solid samples

Benchtop NMR Spinsolve 80 MHz

  • Utilizing a permanent magnet, the Spinsolve 80 MHz operates without the need for liquid helium or nitrogen, simplifying maintenance and reducing operational costs.
  • The system's external hardware lock eliminates the necessity for deuterated solvents, allowing the use of standard protonated solvents without compromising spectral quality.
  • The Spinsolve 80 MHz delivers superb spectral resolution, with linewidths at 50% peak height less than 0.4 Hz, enabling precise analysis of complex mixtures.
  • The spectrometer allows to record 1H and 13C spectrum and has integrated common pulse sequences for most 1D and 2D NMR experiments.
  • Any liquid samples including beverages, dairy products, edible oils, and extracts can be screened for composition in a rapid and reproducible manner.

Instruments specifications:

  • Probe type: 5mm BBI (high sensitivity for 1H)
  • Sensitivity down to μM
  • Analysis time ranges from 5 to 60 min for 1H and up to 24 hours for 13C

SamplePro Tube

  • An automatic liquid handler designed for the high-throughput preparation of liquid samples for NMR analysis

 

 

  • Sample amount depends on the sample type – typically 1 ml for biofluids
  • NMR measurements are recorded by a superuser of the NMR platform
  • NMR datasets are analyzed with the SigMa software and given as relative concentrations (MATLAB or Excel files)
  • Prices apply to samples (biofluids) measured using the laboratory’s SOPs for NMR metabolomics
  • Interpretation and analysis of the results are not included in the fee

 

At the start of a collaboration on research with the University of Copenhagen, a cooperation agreement must be drawn up. There are different types of agreements depending on the nature of the cooperation. See more here: collaboration.ku.dk/cooperation-agreements/

 

 

Assistant Professor Viola Aru, violetta@food.ku.dk

Associate Professor Bekzod Khakimov, bzo@food.ku.dk