LC-MS Platform

Description

The Liquid Chromatography-Mass Spectrometry (LC-MS) platform at the Foodomics Laboratory is based on impact II, LC-QTOF-MS system:

LC-QTOF-MS (impact II, Bruker Daltonics)

The Liquid Chromatography-Quadrupole Time-of-Flight Mass Spectrometer (LC-QTOF-MS) offers high-resolution and high-accuracy mass detection with excellent sensitivity for both targeted and untargeted analyses. It is well-suited for comprehensive profiling of a wide range of metabolites in complex biological samples. The system is integrated with semi-automated LC-MS/MS data processing software (MetaboScape), which efficiently converts complex spectral data into informative metabolite tables ready for multivariate statistical analysis. Additionally, it is equipped with extensive mass spectral libraries, along with in-silico tools such as GNPS and SIRIUS, enhancing metabolite identification and data processing capabilities. The system is widely applicable in fields such as foodomics, metabolomics, and proteomics. It enables the simultaneous detection of both primary and secondary metabolites in complex matrices, including plant materials, food samples, and biological fluids such as blood plasma, urine, and gut contents. Current applications primarily focus on the untargeted molecular profiling of cereals, legumes, fermented foods, dairy products, and beverages such as wine and beer.

Options and requirements for use

 

  • The platform is operated by trained personnel and can be booked by contacting the responsible people.
  • Our experts offer comprehensive support in study design, sample preparation, post-acquisition data processing, and data analysis.
  • The required sample amount for analysis ranges from a few milligrams to a gram, depending on the sample type and the analysis objective.

 

When initiating a research collaboration with the University of Copenhagen, a cooperation agreement must be established. Different types of agreements are available depending on the nature of the collaboration. For more information, visit: collaboration.ku.dk/cooperation-agreements

 

Associate Professor Bekzod Khakimov (bzo@food.ku.dk)

Postdoc Paraskevi Tsermoula (paraskevi@food.ku.dk)