TY - JOUR

T1 - A Multi-Omics Approach Reveals New Signatures in Obese Allergic Asthmatic Children

AU - Gomez-Llorente, Ma Amelia

AU - Martínez-Cañavate, Ana

AU - Chueca, Natalia

AU - Rico, Ma de la Cruz

AU - Romero, Raquel

AU - Anguita-Ruiz, Augusto

AU - Aguilera, Concepcion Ma

AU - Gil-Campos, Mercedes

AU - Mesa, Maria D.

AU - Khakimov, Bekzod

AU - Morillo, Jose Antonio

AU - Gil, Ángel

AU - Camacho, José

AU - Gomez-Llorente, Carolina

PY - 2020

Y1 - 2020

N2 - Background:Asthma is a multifactorial condition where patients with identical clinical diagnoses do not have the same clinical history or respond to treatment. This clinical heterogeneity is reflected in the definition of two main endotypes. We aimed to explore the metabolic and microbiota signatures that characterize the clinical allergic asthma phenotype in obese children.Methods:We used a multi-omics approach combining clinical data, plasma and fecal inflammatory biomarkers, metagenomics, and metabolomics data in a cohort of allergic asthmatic children.Results:We observed that the obese allergic asthmatic phenotype was markedly associated with higher levels of leptin and lower relative proportions of plasma acetate and a member from theClostridialesorder. Moreover, allergic children with a worse asthma outcome showed higher levels of large unstained cells, fecal D lactate and D/L lactate ratio, and with a higher relative proportion of plasma creatinine and an unclassified family member from the RF39 order belonging to the Mollicutes class. Otherwise, children with persistent asthma presented lower levels of plasma citrate and dimethylsulfone.Conclusion:Our integrative approach shows the molecular heterogeneity of the allergic asthma phenotype while highlighting the use of omics technologies to examine the clinical phenotype at a more holistic level.

AB - Background:Asthma is a multifactorial condition where patients with identical clinical diagnoses do not have the same clinical history or respond to treatment. This clinical heterogeneity is reflected in the definition of two main endotypes. We aimed to explore the metabolic and microbiota signatures that characterize the clinical allergic asthma phenotype in obese children.Methods:We used a multi-omics approach combining clinical data, plasma and fecal inflammatory biomarkers, metagenomics, and metabolomics data in a cohort of allergic asthmatic children.Results:We observed that the obese allergic asthmatic phenotype was markedly associated with higher levels of leptin and lower relative proportions of plasma acetate and a member from theClostridialesorder. Moreover, allergic children with a worse asthma outcome showed higher levels of large unstained cells, fecal D lactate and D/L lactate ratio, and with a higher relative proportion of plasma creatinine and an unclassified family member from the RF39 order belonging to the Mollicutes class. Otherwise, children with persistent asthma presented lower levels of plasma citrate and dimethylsulfone.Conclusion:Our integrative approach shows the molecular heterogeneity of the allergic asthma phenotype while highlighting the use of omics technologies to examine the clinical phenotype at a more holistic level.

KW - asthma

KW - gastrointestinal microbiome

KW - metabolomics

KW - multi-omics approach

KW - EXHALED NITRIC-OXIDE

KW - METABOLIC SYNDROME

KW - ADOLESCENTS

KW - DEFINITION

KW - MECHANISMS

KW - PHENOTYPES

KW - MODERATE

U2 - 10.3390/biomedicines8090359

DO - 10.3390/biomedicines8090359

M3 - Journal article

C2 - 32961859

VL - 8

JO - Biomedicines

JF - Biomedicines

SN - 2227-9059

IS - 9

M1 - 359

ER -