A novel method for in Situ detection of hydrolyzable casein fragments in a cheese matrix by antibody phage display technique and CLSM

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

A novel method for in Situ detection of hydrolyzable casein fragments in a cheese matrix by antibody phage display technique and CLSM. / Duan, Zhi; Brüggemann, Dagmar Adeline; Siegumfeldt, Henrik.

In: Journal of Agricultural and Food Chemistry, Vol. 57, No. 21, 2009, p. 10049-10054.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Duan, Z, Brüggemann, DA & Siegumfeldt, H 2009, 'A novel method for in Situ detection of hydrolyzable casein fragments in a cheese matrix by antibody phage display technique and CLSM', Journal of Agricultural and Food Chemistry, vol. 57, no. 21, pp. 10049-10054. https://doi.org/10.1021/jf901026m

APA

Duan, Z., Brüggemann, D. A., & Siegumfeldt, H. (2009). A novel method for in Situ detection of hydrolyzable casein fragments in a cheese matrix by antibody phage display technique and CLSM. Journal of Agricultural and Food Chemistry, 57(21), 10049-10054. https://doi.org/10.1021/jf901026m

Vancouver

Duan Z, Brüggemann DA, Siegumfeldt H. A novel method for in Situ detection of hydrolyzable casein fragments in a cheese matrix by antibody phage display technique and CLSM. Journal of Agricultural and Food Chemistry. 2009;57(21):10049-10054. https://doi.org/10.1021/jf901026m

Author

Duan, Zhi ; Brüggemann, Dagmar Adeline ; Siegumfeldt, Henrik. / A novel method for in Situ detection of hydrolyzable casein fragments in a cheese matrix by antibody phage display technique and CLSM. In: Journal of Agricultural and Food Chemistry. 2009 ; Vol. 57, No. 21. pp. 10049-10054.

Bibtex

@article{4f778e60d50d11dea1f3000ea68e967b,
title = "A novel method for in Situ detection of hydrolyzable casein fragments in a cheese matrix by antibody phage display technique and CLSM",
abstract = "A novel method to monitor in situ hydrolyzable casein fragments during cheese ripening by using immunofluorescent labeling and confocal laser scanning microscopy (CLSM) was developed. Monoclonal single chain variable fragments of antibody (scFvs) were generated by antibody phage display toward three small synthetic peptides of the alpha(s1)-casein sequence. These peptides traverse enzymatic cleavage sites of casein during cheese ripening. The specificity of the generated anti-peptide antibodies was determined by ELISA and Western blot. Finally, an immunofluorescent labeling protocol was successfully developed for the detection of scFvs binding to different alpha(s1)-casein fragments inside a cheese matrix by CLSM. To our knowledge, this is the first demonstrated immunofluorescent labeling method for in situ analysis of proteolysis phenomena in the cheese matrix. Additionally, this technique offers a high potential to study in situ dynamic spatial changes of target components in complex food systems.",
author = "Zhi Duan and Br{\"u}ggemann, {Dagmar Adeline} and Henrik Siegumfeldt",
year = "2009",
doi = "10.1021/jf901026m",
language = "English",
volume = "57",
pages = "10049--10054",
journal = "Journal of Agricultural and Food Chemistry",
issn = "0021-8561",
publisher = "American Chemical Society",
number = "21",

}

RIS

TY - JOUR

T1 - A novel method for in Situ detection of hydrolyzable casein fragments in a cheese matrix by antibody phage display technique and CLSM

AU - Duan, Zhi

AU - Brüggemann, Dagmar Adeline

AU - Siegumfeldt, Henrik

PY - 2009

Y1 - 2009

N2 - A novel method to monitor in situ hydrolyzable casein fragments during cheese ripening by using immunofluorescent labeling and confocal laser scanning microscopy (CLSM) was developed. Monoclonal single chain variable fragments of antibody (scFvs) were generated by antibody phage display toward three small synthetic peptides of the alpha(s1)-casein sequence. These peptides traverse enzymatic cleavage sites of casein during cheese ripening. The specificity of the generated anti-peptide antibodies was determined by ELISA and Western blot. Finally, an immunofluorescent labeling protocol was successfully developed for the detection of scFvs binding to different alpha(s1)-casein fragments inside a cheese matrix by CLSM. To our knowledge, this is the first demonstrated immunofluorescent labeling method for in situ analysis of proteolysis phenomena in the cheese matrix. Additionally, this technique offers a high potential to study in situ dynamic spatial changes of target components in complex food systems.

AB - A novel method to monitor in situ hydrolyzable casein fragments during cheese ripening by using immunofluorescent labeling and confocal laser scanning microscopy (CLSM) was developed. Monoclonal single chain variable fragments of antibody (scFvs) were generated by antibody phage display toward three small synthetic peptides of the alpha(s1)-casein sequence. These peptides traverse enzymatic cleavage sites of casein during cheese ripening. The specificity of the generated anti-peptide antibodies was determined by ELISA and Western blot. Finally, an immunofluorescent labeling protocol was successfully developed for the detection of scFvs binding to different alpha(s1)-casein fragments inside a cheese matrix by CLSM. To our knowledge, this is the first demonstrated immunofluorescent labeling method for in situ analysis of proteolysis phenomena in the cheese matrix. Additionally, this technique offers a high potential to study in situ dynamic spatial changes of target components in complex food systems.

U2 - 10.1021/jf901026m

DO - 10.1021/jf901026m

M3 - Journal article

C2 - 19831397

VL - 57

SP - 10049

EP - 10054

JO - Journal of Agricultural and Food Chemistry

JF - Journal of Agricultural and Food Chemistry

SN - 0021-8561

IS - 21

ER -

ID: 15920196